聚合酶

The ISSR fingerprinting of 18 Japanese plum cultivars and 12 plum species or variety were established by using optimum system with primer 5"-9T-3"and 5"-9C-3", respectively. The identification rate of plum cultivars and species or variety was 100%. The effects of five important components on reaction of SSR were studied with Prunus salicina cv. Meilili. The results showed that the optimum concentration of five important components i.e. Tag DNA polymerase, Mg +, single primer, template DNA and dNTPs in 25 μL reaction system of SSR were 1.5u, 2.0mM, 0.8 μ M, 30~40ng and 0.16~0.24mM, respectively. The dendrograms of these cultivars was contructed according the Jaccard coefficient of similarity.

利用该优化体系，以5′-_9T-3′为引物，构建了中国李18个品种的ISSR指纹图谱，该引物可将这些品种完全区别开来；以5′-_9C-3′为引物，构建了李属6类种质资源的ISSR指纹图谱，该引物区分率为100％。4、中国李品种适宜的SSR反应分析体系是：25μL SSR反应体系中，Taq DNA聚合酶、Mg~(2+)、每个引物、模板DNA和dNTPs等5种成分的适宜浓度分别是：1.5u、2.0mmol·L~(-1)、0.8μmol·L~(-1)、30～40ng和0.16～0.24mmol·L~(-1)。5、采用RAPD、ISSR和SSR等三种分子标记技术对中国李品种资源54份和2个欧洲李品种进行了遗传多样性分析，并以品种间的遗传相似系数构建了Jaccard聚类树状图。

After 8 weeks,PDGF-B measured with reverse transcription polymerise chain reaction and pathologic alter were observed with HE staining.

高脂喂养6周后以球囊拉伤方法建立兔颈总动脉内皮损伤模型。8周时处死动物，以逆转录聚合酶链式反应法检测动脉损伤处PDGF-B mRNA表达，HE染色观察颈动脉组织病理形态学改变。

Methods Polymerise chain reaction and restriction fragment length polymorphism were used to analyze the polymorphisms of MTHFR genes C6677T and A1298C in 56 patients of colorectal cancer and 143 age-and-sex-matched control subjects.

方法以聚合酶链反应和限制性片段长度多态方法，对56例直肠癌患者和143例正常人对照的MTHFR基因C677T和A1298C基因型进行检测分析其对直肠癌风险的相关性。结果在正常对照组中，MTHFRC677TCC、CT、TT基因型频率分别为62·90%、35·08%和2·12%，而在直肠癌病人中分别为58·93%、26·77%和14·26%。A1298C与A1298C基因型在病例和对照中的分布差异无显著性意义。

Methods:Semi-quantitative reverse transcriptase polymerase chain reaction was used to examine the expression of MRP gene in 65 bone marrow aspirates of AL patients and 15 normal volunteers peripheral blood mononuclear cells.

应用半定量逆转录-聚合酶链反应，检测了65例AL患者骨髓和15名正常人外周血单个核细胞中MRP基因的表达。

Many commercial laboratories offer their own PCR assays, which are not subject to strict independent quality controls.

许多商业实验室提供自己的聚合酶链反应检测，这是没有受到严格的独立的质量控制。

Objective: A novel method for 5'-overhang blunting with Pfu DNA polymerase was reported in this paper.

目的：报道一种利用Pfu DNA聚合酶延伸法补平限制片段5'-突出末端的平端化方法。

The IGF-ⅠmRNA and GPx mRNA in the liver of carps were determined with RT-PCR.

采用反转录一聚合酶链式反应对肝脏中的IGF-Ⅰ和GPx mRNA的表达进行测定。

Methods: Using reverse transcription-polymerase chain reaction,cavies lung tissue Eotaxin mRNA expression were given semi-quantitative analysis.

采用逆转录-聚合酶链式反应法半定量分析哮喘豚鼠肺组织嗜酸性粒细胞趋化因子mRNA表达水平的变化。

Methods Expressions of TGF -β1 mRNA and its receptors were detemined in villi and deciduas by semi-quantitative reverse transcriptional-polymerase chain reaction from 38 cases of spontaneous abortion women and 35 cases of normal early pregnancy women.

应用半定量逆转录-聚合酶链反应技术，检测 38 例 RSA 患者和 35 例正常早孕妇女的绒毛及蜕膜组织中 TGF -β1 mRNA，TβRmRNA 的表达情况。

Methods 605 cases with stroke and 313 healthy controls were recruited in this study, and the stroke group was delaminated into two subgroups according to the history of hypertension. SNP rs1800780, an A to G base change located in intron 12 of the gene, was used as a genetic marker.

以rs1800780位点为遗传标记，采用聚合酶链反应和限制性片断长度多态性，检测605例缺血性卒中患者和313例对照组人群NOS3基因的多态性。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。