聚合酶

Hepatitis B virus is the smallest DNA virus which is a member of the viral family Hepadnavirida. It uses its DNA as template and the RNA polymerase II of the host cells to transcribe its viral transcripts. There are four major RNAs transcribed from HBV genome. All of the four major transcripts are unspliced, terminated at a common site, polyadenylated at the 3' terminal and capped at the 5' terminal.

B 型肝炎病毒属於肝病毒科，为目前已知最小的去氧核醣核酸病毒，利用其病毒的去氧核醣核酸以及宿主细胞内的核醣核酸聚合酶可转录出四种主要的核醣核酸产物，此四种主要的转录产物均未经剪接并终止於核心开放编阅序列的共同polyadenylation 讯号，且在其5'端均经过capping修饰。

In this study we constructed the recombinant pcDNA-HA-rpb in E.coli cells and we expressed the three polymerase II subunits; Rpb5, Rpb6 and Rpb7 as HA-tagged polypeptides by transfection in 293T mammalian cells, as well as untagged nuclearβ-actin.

在这项研究中，我们在大肠杆菌中构建了pcDNA-HA-rpb重组质粒，表达三个聚合酶II亚基： Rpb5 ， Rpb6和Rpb7，通过转染哺乳动物细胞293T，从而表达带HA标签的多肽，同时转染不带标签的β-actin。

Methods 200mL fresh midpiece urina sample was collected from each of the 53 patients of transitional cell carcinoma of bladder, 20 patients of other uropoiesis disease, and 10 healthy volunteers, expression of Survivin was detected by nested RT-PCR. Voided urine cytology and cystoscopy were also conducted.

留取53例膀胱移行上皮癌、20例泌尿系其他疾病患者及10例健康志愿者新鲜中段晨尿200mL，对53例膀胱移行上皮癌患者术后癌组织，用巢式逆转录聚合酶链反应(nested RT-PCR)检测Survivin的表达，同时行尿脱落细胞学检查及膀胱镜取材病检。

METHODS Immuno-in situ PCR was used for detection of HCMV antigen in pregnant women cervix uteri exfoliated cells.

方法应用原位免疫聚合酶链反应技术检测早、中、晚期孕妇宫颈脱落细胞中巨细胞病毒抗原。

Objective To establish a sensitive, simple to use and low noise nested/multiplex PCR for simultaneously detection of Plasmodium falciparum and Plasmodium vivax.

摘要目的建立简便、灵敏、低本底、可同时检测恶性疟原虫和间日疟原虫的套式/多重聚合酶链反应系统。

A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物，自接种H5N1亚型病毒的鸡胚组织中提取RNA，反转录后采用高可信度DNA聚合酶经PCR扩增M1基因，采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达，纯化获得N末端携带多聚组氨酸标签的重组蛋白，分子量约29.8 kDa。

Of the polymerase and allows it to proceed. The octamer then binds to the DNA behind the polymerase and no longer presents an obstacle to progress.

图27.36：RNA聚合酶在前进中会取代DNA的组蛋白八聚体，DNA恢复成环并附着，组成一个狭窄的环。

Bacteria genomic DNA was extracted to do polymerase chain reaction to amplify specific DNA and then to assess coden sequence.

萃取细菌DNA后利用聚合酶连锁反应将细菌特定的DNA片段增幅，定序比对标准序列判定基因型。

The cDNA squence of antibiotic, an antibacterial peptide, a process for preparing the said cDNA and a process for recombining the antibacterial peptide are disclosed. The present frequency of amino acid codens in Attacin A cDNA is analyzed. The high-frequency coden is introdued to its protein sequence to configure its cDNA sequence. The target gene is synthesized by chemical synthesis method or polymerase chain reaction.

本发明公开了葛佬素的cDNA序列，葛佬素cDNA的制备方法以及重组抗菌肽葛佬素的制备方法；通过对同种已知蛋白质Attacin A cDNA中氨基酸密码子的出现频度进行分析，采用出现频度高的密码子带入葛佬素蛋白质序列中进行葛佬素的cDNA序列构建，用化学合成法及聚合酶链式反应进行目的基因的合成，另外，采用基因重组法制备抗菌肽葛佬素，并测定了其活性。

It was discovered that trimalonic acid C60、fullerol、dimalonic[60]fullerene hydrolysate、tetraethyl methano[60]fullerenediphosphonate、methano[60]fullerenediphosphonic acid and dimalonic acid C60 exhibited different inhibitory effects on Taq DNA polymerase.

实验发现，6种富勒烯衍生物三丙二酸富勒烯、富勒醇、富勒烯丙二酸水解物、亚甲基富勒烯二膦酸四乙酯、亚甲基富勒烯二膦酸四乙酯的完全水解物和二丙二酸富勒烯对Taq DNA聚合酶存在不同程度的抑制作用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。