聚合酶

Recent studies have shown that a viral RNA polymerase known as PB2 is critical for efficient influenza transmissibility.

最近的研究表明，病毒的RNA聚合酶PB2在流感病毒有效传播中起关键作用。

Rats were sacrificed at 0, 6, 12, 24 and 48 hours after reproduction of pancreatitis in both groups. The intestinal mucosal permeability was assessed with 99mTechnetium Diethlene Triamine Pentacetic Acid method, and the expression of substance P was evaluated with reverse transcriptase polymerase chain reaction and Western blotting analysis.

分别于制模成功后0、6、12、24和48 h处死两组大鼠，并留取标本；利用同位素法测定肠黏膜通透性，分别用逆转录-聚合酶链反应和蛋白质免疫印迹法检测P物质在肠组织中的mRNA和蛋白表达水平。

Objective To observe the unique DNA profile and the relationship between DNA profile and pheno type of Trichophyton rubrum , and establish an effective molecular biologic method to identify T. Rubrum.

目的 用聚合酶链反应扩增红色毛癣菌和须癣毛癣菌临床分离株的DNA，观察PCR指纹的差异以及基因型与传统表型的关系，建立一种红色毛癣菌分子生物学鉴定方法。

Mitochondrial 16S rRNA gene fragments were amplified from dolphins Tursiops truncatus,Sousa chinensis and Steno bredanensis using PCR technique.

采用聚合酶链式反应技术扩增瓶鼻海豚、中华白海豚和糙齿海豚的线粒体DNA16S rRNA基因，获得了大约600bp的片段。

Mitochondrial 16S rRNA gene fragments were amplified from dolphins. Tursiops truncatus , Sousa chinensis and Steno bredanensis using PCR technique. The PCR products were about 600 bp and were directly sequenced subsequently.

摘 要：采用聚合酶链式反应技术扩增瓶鼻海豚、中华白海豚和糙齿海豚的线粒体DNA 16S rRNA基因，获得了大约600 bp的片段。

Whereas, in unactivated mouse spleen T lymthocytes, no binding could be found. Actin was closely associated to BAF complex and RNA Polymerase Ⅱ, but not so closely to NF1/CTF.

肌动蛋白与BAF复合物和RNA聚合酶Ⅱ的联系紧密，与转录因子NF1／CTF的联系不太密切。

The genomic DNA of Unio douglasiae, collected from Suya Lake, Henan Province, was used as template DNA to optimize the RAPD-PCR reaction conditions including the concentration of DNA template, random primer, dNTPs, Mg2+ and Taq DNA polymerase and the annealing temperature for thermal cycle.

以河南宿鸭湖圆顶珠蚌的基因组DNA为模板，对淡水蚌类RAPD反应体系中的模板DNA浓度、随机引物浓度、dNTPs浓度、Mg~(2+)浓度、Taq DNA聚合酶浓度和和热循环参数中的退火温度进行了优化。

Three SNPs (rs3753118, rs3753116 and rs7830490) of ASAH1 in 254 schizophrenia trios were genotyped by using PCR-RFLP, and the LD, TDT and haplotype TDT were analyzed by using EMLD, UNPHASED and TRANSMIT softwares.

应用聚合酶链反应和限制性片段长度多态性技术对254个精神分裂症核心家系ASAH1基因的3个SNPs（rs3753118、rs3753116和rs7830490）进行检测，计算LD、TDT及单体型TDT。

Three SNPs (rs3753118, rs3753116 and rs7830490) of ASAH1 in 254 schizophrenia trios were genotyped by using PCR-RFLP, and the LD, TDT and haplotype TDT were analyzed by using EMLD, UNPHASED and TRANSMIT softwares.

应用聚合酶链反应和限制性片段长度多态性技术对254个精神分裂症核心家系ASAH1基因上的3个SNPs（rs3753118、rs3753116和rs7830490）进行检测，计算连锁不平衡、传递不平衡及单体型TDT。

Coli. Methods Among 95 unrepeated strains isolated from clinic, 38 positive strains tested by ESBLs confirmatory test were collected from HAI and CAI subjected to detection for drug resistant gene TEM, SHV, CTX-M-1 and for disinfectant resistant gene qacE△l-sull by polymerase chain reaction.

收集从临床标本分离的大肠埃希菌95株，经ESBLs确证试验将其中38株阳性菌株分为医院与社区获得性感染2组，用聚合酶链反应检测耐药基因TEM、SHV、CTX-M-1以及耐消毒剂基因qacE△1-su l1。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。