聚合酶

Porcine mature protein IL-18 gene was amplified by RT-PCR from porcine splenocyte total RNA extracts and then was cloned and sequenced.

采集6月龄河南良杂猪的脾脏，分离淋巴细胞后直接提取总RNA，进行反转录－聚合酶链反应扩增，扩增产物进行T-A克隆、测序，获得了河南良杂猪IL-18基因成熟蛋白序列。

Objective To establish a sensitive and specific gene probe method for detection of Staphylococcal enterotoxin B gene.

目的 建立一种灵敏度高、特异性好的DNA 探针检测葡萄球菌肠毒素B 基因方法。方法用聚合酶链反应扩增的方法进行葡萄球菌B 型肠毒素基因探针生物素标记，用制备好的基因探针，进行斑点杂交，对葡萄球菌肠毒素10 株标准株和39 株临床株分别进行了检测。

By the method of polymerase chain reaction-single strand conformation polymorphism, this paper studied the genetic polymorphism of exon 1 and intron 1 in the aquaporin gene of seven species of Stipa in the meadow steppe, typical steppe, and desert steppe of Inner Mongolia.

以分布于内蒙古的草甸草原、典型草原、荒漠草原上的7种针茅属植物为材料，利用聚合酶链反应－单链构象多态性方法，分析了水孔蛋白基因的外显子1和内含子1的遗传多态性。

METHODS The minimal effective bactericidal concentration of 60 strains of Gram negative bacteria resistant to QACs was detected by diluting-counting method; the phenotype to QACs by the improved phenotype screening test, the qacE-sull gene of resistance to QACs and sulphonamide compounds by PCR.

方法使用稀释计数法测定60株革兰阴性杆菌对季胺盐复合物的最低有效杀菌浓度，利用改良的表型筛选试验检测耐QACs的表型，采用聚合酶链反应试验检测耐季铵盐消毒剂-磺胺复合物基因qacE-sul1。

Methods:The minimal effective bactericidal concentration of 60 strains Gram negative bacterium resistant to QACs were detected by diluting-counting method; the phenotype to QACs by the improved phenotype screening test,the qacE-sul1 gene of resistance to QACs and Sulphonamide Compou...

使用稀释计数法测定60株革兰阴性杆菌对季胺盐复合物的最低有效杀菌浓度，使用改良的表型筛选试验检测耐QACs的表型，使用聚合酶链反应试验检测耐季铵盐消毒剂-磺胺复合物基因qacE-sul1。

Methods: For 4 tetrameric STR loci (D5S818, D8S1179, D18S51and D21S11) genetic research, monoplex PCR methods were established to study the allele frequency and the applicability of forensic medicine.

应用聚合酶链反应，聚丙烯酰胺凝胶电泳及银染分型技术，对延边地区109名朝鲜族无关个体进行群体调查，获得四种基因座的群体遗传学资料。

The commercial UNG and thermostable DNA polymerases are all expensive.

但是，无论UNG还是热稳定的DNA聚合酶，国外产品都十分昂贵。

On other hand, specific fragments containing thermostable DNA polymerase gene were amplified from the genomic DNA of some strains of thermophilic bacteria isolated from Yunnan hot springs, and recombinant plasmids were constructed.

同时，我们还利用特异性的热稳定DNA聚合酶基因的扩增引物，从生长在云南温泉的嗜热菌总DNA中筛选出了能被此类引物扩增的特异性片段，并构建了相应的重组质粒。

Full-length plasmid DNA was amplified with the above primers with a high-fidelity thermostable DNA polymerase capable of long-rang amplification.

用能扩增长片段的高保真耐热 DNA聚合酶扩增全长的质粒DNA，直接转化大肠杆菌。

Methods One hundred thirty PCOS women and one hundred seventy five normal women as controls were enrolled in this study. The genotypes were screened by polymerase chain reaction-On/off switch and the product was isolated by electrophoresis on a 2.5% agarose gel containing ethidium bromide and visualized using an ultraviolet transilluminator.

应用On/off Switch-聚合酶链反应及琼脂糖凝胶电泳分离技术分析130例多囊卵巢综合征患者及175例对照组妇女TNF-α基因rs3179060C/A多态，比较rs3179060C/A两组等位基因及基因型与PCOS及其高雄激素之间的关系。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。