聚合酶

Coil O85 antigen contained 8 varieties of genes, including UDP-Nacetylglucosamine-2-epimerase gene (f1). UDP-galactopyranose mutase gene, glycosyl transferase genes (f3, f5, f6, f8), O-antigen transferase gene and O-antigen polymerase gene, in which two genes, wzx and wzy and 4 pairs of primers were identified to be specific to E.

发现8个开放阅读框架并确定功能，分别为：UDP-N-乙酰葡萄糖－2-异构酶基因，吡喃型UDP-半乳糖变位酶基因，糖基转移酶基因（orf3、orf5、orf6和orf8），O-抗原转运酶基因和O-抗原聚合酶基因。

Rapid amplification of cDNA end:Retroverse transcription product of total RNA extracted from normal porcine tissue was used as the template,gene specific primers were designed and advantage 2 polymerase mix was used in PCR,of which using porcine genomic DNA as the template:forward primer was designed according to the acquired consensus region of human and pig FGL2 3′ sequences while reverse primer was designed from human FGL2 3′ end downstream sequence;TA cloning.

以猪正常小肠及心脏组织提取新鲜总RNA，反转录后作为模板，设计基因特异性引物，采用Advantage 2 聚合酶混合物进行PCR扩增；依据猪与人FGL2基因3′端已知同源序列设计PCR上游引物，以人FGL2基因3′末端序列设计下游引物，以猪基因组DNA为模板采用Advantage 2 聚合酶混合物进行PCR反应；PCR载体重组质粒DNA亚克隆扩增。

Current advance of Taq DNA polymerase improvement and application in medicine has been discussed in this paper.

本文介绍了Taq DNA聚合酶的结构与功能改造的研究现状，并展望了Taq DNA聚合酶在医药领域的应用前景。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

It has been speculated that the archaeal polymerase resembles the ancestor of the specialized eukaryotic polymerases.

有推测指古细菌聚合酶与专门的真核生物聚合酶相似。

DNA polymerases are capable of editing and error correction, but RNA polymerases do not appear to have this capacity.

DNA聚合酶可以校对和修正，但是 RNA 聚合酶没有这种能力。

Each of the three RNA polymerases contains 12 or more subunits, some of which are similar to those of E. coli RNA polymerase.

原来译文：三种RNA聚合酶的每一种含有12个或更多亚基，其中有些亚基和大肠杆菌RNA聚合酶中的相似。

The results will help us to further comprehend the mechanism of RNA polymerase transcription, the way of transcription processing and transportation, and the structural and functional relationship among the three RNA polymerases.

研究结果对于人们进一步了解RNA聚合酶Ⅲ的转录机制、加工和运输过程及RNA聚合酶之间的结构与功能关系等均具有重要的意义。

Other people think the pclymerase they are small molecules in the fusion replications of viruses by setting pclymerase.

也有人认为是聚合酶，他们是通过释放聚合酶影响病毒融合复制过程的小分子。

Among analyzed 197 synthetic genes on chip, approximately 60 genes have been expressed, including 18 two-component/kinase, 10 glycolysis/TCA, 3 gene clusters, 7 regulators, 5 asaption/celldivision/differentiation/sporulation, 4 transport/binding proteins, 2 chaperones, 5 RNA polymerase sigma-factors, 3 transcription/translations, 2 fatty acid synthesises and 1 RNA polymerase β subunit.

对芯片上的197个合成基因进行分析：大约有60个基因进行了表达，其中包括18个双组分或激酶基因；10个糖酵解或者三羧酸循环途径基因；3个阿维基因簇；7个调节基因；5个细胞分化或孢子形；4个运输或固定基因；两个伴侣分子；5个RNA聚合酶σ因子；3个转录基因；两个脂肪酸合成基因；1个RNA聚合酶β亚基。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。