聚合酶

Methods The DNA polymorphism was analyzed in 50 individuals of Macaca mulatta and 50 individuals of Macaca irus using 15 PCR-microsatellites DNA loci and the differences of allele numbers between those two species were compared.

利用聚合酶链反应扩增技术采用15个多态性微卫星DNA位点对50只恒河猴和50只食蟹猴个体进行了DNA多态性的分析，对比两群体间等位基因数目差异。

Methods: The expression of Livin mRNA was measured in 91 AL and 17 chronic myelocytic leukemia patients by semi-quantity reverse transcription polymers chain reaction.

采用半定量逆转录-聚合酶链反应检测91例急性白血病和17例慢性粒细胞性白血病患者Livin mRNA的表达水平， 21名健康人的标本作正常对照。

Methods: The expression of Livin mRNA was measured in 91 AL and 17 chronic myelocytic leukemia patients by semi-quantity reverse transcription polymers chain reaction. 21 healthy adults were also measured as normal control.

采用半定量逆转录-聚合酶链反应检测91例急性白血病和17例慢性粒细胞性白血病患者Livin mRNA的表达水平， 21名健康人的标本作正常对照。

Methods The expression of CD87 was detected with flow cytometry in 54 AML patients and 20 acute lymphoblastic leukemia patients. The expressionsa of CD87 on U 937, HL-60 and K562 cells were also studied with FCM and reverse transcription-polymerase chain reaction during differentiation induced by phorbol myristate acetate in vitro.

应用流式细胞术检测了54例急性髓性白血病及20例急性淋巴细胞白血病患儿CD87的表达，还用FCM及逆转录-聚合酶链反应法检测了U937、HL-60、K562细胞在氟波酯作用下诱导分化过程中CD87表达的改变。

Objective To evaluate the diagnostic and therapeutic values of two non invasive test methods for Neisseria gonohoeae.

目的探讨非侵入性取材的尿沉渣培养和尿沉渣聚合酶链反应－杂交梳法检测淋球菌在诊断与治疗中的意义。

Ovarian neoplasma ; Multiple drug ; Resistance ; Gene expression ; Polymerase chain reaction

卵巢肿瘤；抗药性；多药；基因表达；聚合酶链反应

Methods From January to March 2007, cases from both outbreaks and sporadic episodes of acute nonbacterial gastroenteritis were investigated in Beijing, and the fecal specimens of the patients were collected.

方法收集北京市2007年1—3月非细菌性胃肠炎暴发和散发病例，采集患者粪便标本，使用逆转录聚合酶链反应对粪便标本进行诺如病毒RNA检测，对RT—PCR阳性标本的PCR产物进行克隆测序。

In contrast, exposure to ectopically expressed noncanonical Wnt11 markedly decreased the expression of Oct-4 and Nanog and induced mRNA expression (quantitative real-time reverse-transcription polymerase chain reaction) of cardiac-specific genes (Nkx2.5, GATA-4, atrial natriuretic peptide,- and -myosin heavy chain, and cardiac troponin T) by day 3 with subsequent progression to a pattern characteristic of the cardiac fetal gene program.

对比之下，接触到非典型wnt11能明显降低Oct-4 和 Nanog表达和在第3天出现诱导心脏特异性基因（Nkx2.5，GATA - 4，心房利钠肽，- and -肌球蛋白重链，肌钙蛋白T）mRNA的表达（应用反转录聚合酶链式反应实时定量），随后逐渐表达为心脏胎儿基因。

To investigate distribution of gene mutations in LPL exon 9 in Chinese population and to study the possible effects of the mutation on plasma lipids, variants were screened by PCR-SSCP and DNA sequencing. Ser~(447)→stop polymorphism was identified by PCR-RFLP . the plasma lipids in stop447 carrier group and stop447 noncarrier group were compared and analysis.

为探讨中国人群LPL基因外显子9常见突变的种类、频率以及其可能产生的影响，我们利用聚合酶链反应-单链构象多态性分析和DNA测序技术及PCR-RFLP技术检测出我国人群的LPL外显子9基因突变、基因型和等位基因频率，确定了中国人群Ser~(447)→stop这一多态性位点。

Reverse transcription polymerase chain reaction was used to examine the deletions at P16 and FHIT in 52 patients with lung cancer and 9 patients with nonmalignant disease.

采用逆转录——聚合酶链反应方法，对52例肺癌患者和9例肺良性疾病患者的纤支镜活检标本中P16和FHIT基因缺失情况进行检测。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。