聚合酶

Objective:Through observing the positive detect rate of hemoculture, Widal reaction,lipopolysaccha-ride passive hemagglutination assay and fluorescent quantitation polymerase chain reaction in those fever patients who have highly suspected infectious with salmonella paratyphi A.We explored the clinical value of early diagnosis of fluorescent quantitation PCR regarding paratyphoid A and evaluated its specificity,sensitivity and repeatability.

目的：通过观察血培养、Widal reaction、脂多糖—被动血凝试验及荧光定量聚合酶链反应在临床高度怀疑为甲型副伤寒沙门菌感染发热患者中的检出阳性率，初步探讨荧光定量PCR早期诊断甲型副伤寒的临床价值，并评价荧光定量PCR特异性、敏感性和可重复性。

Was used to cons Methods: The pSUPER plasmid cloned into RNA polymeraseⅢ H1 promoter was used to construct new vectors which can code hereditable shRNA aimed to HPV18E6/E7 gene mRNA.

采用克隆有人Ⅲ型RNA聚合酶H1启动子的pSUPER质粒，设计并构建针对HPV18E6、E7基因mRNA的、可稳定遗传的编码发夹状结构的特异性RNA干扰质粒载体。

Histiocytic disorders ; malignant ; Polymerase chain reaction ; Receptor ; antigen ; T-cell ; Gene rearrangement

组织细胞增生症；恶性；聚合酶链反应；受体；抗原； T细胞；基因重排

One histiocytic sarcoma showed t(14;18) by quantitative-polymerase chain reaction, which was confirmed by fluorescence in situ hybridization analysis showing IGH/BCL2 fusions in neoplastic histiocytes.

定量性聚合酶链反应显示1例组织细胞肉瘤发生了t(14；18)，此结果也得到荧光原位杂交分析的证实，即肿瘤性的组织细胞发生了IGH/BCL2基因的融合。

The histochemical staining and polymerase chain reaction was used to detect oral leukoplakia.

应用组织化学染色法和聚合酶链反应技术对口腔白斑是否存在念珠菌进行检测。

Methods Homozygous deletion of p16/MTS1 gene was examined by PCR methods in 68 cases of colorectal carcinoma.

方法采用聚合酶链反应方法，对68例结直肠癌组织中p16/MTS1基因纯合缺失进行检测。

METHODS The animal model was induced by injecting suspension of yeast hypodermically.

方法用酵母混悬液复制发热大鼠模型，采用实时荧光定量聚合酶链反应检测大鼠下丘脑中COX-1、COX-2的mRNA表达的变化。

In Picomaviridae, 3D protein was RNA dependent RNA polymerase, and had been applied indifferential diagnosis of FMDV infection, and other picornaviruses.

在PTV病毒中，3D蛋白是具有RNA依赖RNA聚合酶(RNA dependent RNA polymerase，RDRP)活性蛋白，能介导机体产生特异性的抗体。

Methods Reverse transcription polymerase chain reaction method was adopted in detecting VEGF-CmRNA expression in 60 cases of breast infiltrative tubular cancer.

方法用逆转录聚合酶链反应法检测60例乳腺浸润性导管癌VEGF-C mRNA的表达情况，取15例乳腺纤维腺瘤标本作为对照。

In nuclear style families, to study the genetic association and linkage relationship and to provide the genetic basis information for the further research. Methods: We investigated the genetic structure and relationship of 116 Keshan Disease patients and 65 local normal people in Shaanxi province with HLA-DRB1 locus, which is characterized by high polymorphism and inheritage stability, with polymerase chain reaction-sequence specific oligonuclotide probe.

本研究选用具有高度多态信息量及遗传稳定性的人类HLA-DRB1遗传位点，以陕西118名克山病人及当地65名正常对照人群作为研究对象，用聚合酶链反应—序列特异性寡核苷酸探针的方法对其遗传结构及其相互关系进行了分析研究，并在核心家系中采用基于单体型的相对风险及传递不平衡检验对其遗传指标进行了统计计算。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。