聚合酶

Objective To evaluate the nested polymerase chain reaction technique in diagnosing endobronchial tuberculosis.

目的 了解巢式聚合酶链反应爱情技术对支气管内膜结核的诊断癌症价值。

Methods Immunohistochemical staining and reverse transcription-polymerase chain reaction were used to detect HLA-DR protein and mRNA expression in 36 cases of gastric carcinoma and 36 cases of normal gastric epithelia.

采用免疫组织化学和逆转录聚合酶链反应检测36例胃癌及正常胃黏膜上皮中HLA-DR抗原的表达情况，并分析其表达与胃癌临床病理特征的关系。

Methods The mRNA level of tissues of 39 cases of breast cancer and 14 cases of fibroadenoma was measured by RT-PCR technique and its correlation was analyzed.

抽取乳腺肿瘤组织总RNA，采用半定量逆转录－聚合酶链反应检测39例乳腺癌和14例乳腺纤维腺瘤组织中RECK-mRNA和MMP9-mRNA的表达，分析其关系。

Objective To investigate the relationship between the expression of poly ADP-ribose polymerase 1(PARP-1) and the genesis and development in human breast fibroadenoma.

目的 研究聚ADP核糖聚合酶1（PARP-1）在人类乳腺纤维腺瘤中的表达，探讨纤维腺瘤的发生发展机制。

Polymerase chain reaction ; Fluorogenic quantitative ; OATP-E ; Gene expression

聚合酶链反应；荧光定量； OATP-E ；基因表达

Liver disease ; alcoholic ; Low molecular weight polypeptide7; Carbonyl stress ; Polymerase chain reaction ; Fluorogenic quantitative

酒精性肝病；低分子量多肽7；羰基应激；聚合酶链反应；荧光定量

The expressions of t bet and gata 3 mrna and protein in the lungs were detected by reverse transcriptase polymerase chain reaction and western blot respectively.

用逆转录聚合酶链反应测定法和western blot法分别检测t bet， gata 3 mrna和蛋白在肺组织的表达。

Gerbillinae ; DNA ; Polymerase chain reaction ; Genetics

沙鼠亚科； DNA ；聚合酶链反应；遗传

Members of the metabolically diverse genus Gordonia were isolated from various biotopes including pristine and polluted sites around Taiwan.

本研究为加速环境中Gordonia菌种之筛选，利用16S rRNA基因作为标的，设计Gordonia菌属特异性引子，并配合聚合酶连锁反应之快速侦测技术鉴定此类放线菌。

The PCR based assay was tested on 19 strains isolated in throats from patients with guttate psoriasis. The results demonstrated that 6 strains were positive in 15 streptococus strains among which there were 3 streptococus pyogenes, 1.S.agalactiae, 1 S. eguisimilis and 1 S. uberis, and 4 non- streptococus strains were negative. Emm6 was found not only in suppurative hemolytic streptococus, but also in S.uberis.

应用聚合酶链式反应方法对点滴型银屑病患者咽部培养出的19株细菌中的M6蛋白基因进行检测，结果，15株链球菌中6株为阳性，其中化脓链球菌3株，无乳链球菌、似马链球菌和乳房链球菌各1株，4株非链球菌均为阴性。M6蛋白基因不仅存在化脓性溶血链球菌中，在其它链球菌如乳房链球菌中也存在。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。