聚合酶

Imatinib, a BCR-ABL tyrosine kinase inhibitor, is able to induce a complete cytogenetic response in more than 85% of patients with CML. However, the researchers note that patients with a complete cytogenic response tend to relapse when imatinib is discontinued, and less than 10% of patients achieve a molecular remission, defined by an undetectable residual disease using real-time quantitative–polymerase chain reaction.

Imatinib是一种BCR-ABL酪胺酸激酶抑制物，可以在85%以上的CML病患诱导完整的细胞生成反应；不过，研究者指出，完整细胞生成反应的病患倾向在停用imatinib时复发，不到10%的病患达到分子生物学上的缓解，其定义为以即时定量聚合酶连锁反应侦测不出残余疾病。

Imatinib, a BCR-ABL tyrosine kinase inhibitor, is able to induce a complete cytogenetic response in more than 85% of patients with CML. However, the researchers note that patients with a complete cytogenic response tend to relapse when imatinib is discontinued, and less than 10% of patients achieve a molecular remission, defined by an undetectable residual disease using real-time quantitative–polymerase chain reaction.

Imatinib是一种BCR-ABL酪胺酸激酶抑制物，可以在85%以上的CML病患诱导完整的细胞生成反应；不过，研究者指出，完整细胞生成反应的病患倾向在停用imatinib时復发，不到10%的病患达到分子生物学上的缓解，其定义为以即时定量聚合酶连锁反应侦测不出残餘疾病。

In sham-operation group,animals were decollated and their brains were taken out at 24 hours postoperatively. In all the groups, hippocampal brain injury was detected using histopathological method combined with semi-quantified RT-PCR methods for detecting caspase-3 mRNA. Adult rats aged 90 days were used in modified y maze to examine their learning and memory functions. All these three experiments were independent.

各组海马区脑损伤行组织病理学检测，半胱氨酸天冬氨酸蛋白酶-3 mRNA的表达采用半定量反转录-聚合酶反应方法进行检查，成年90 d龄的大鼠进行三等分迷宫测试其学习记忆能力，3个实验各自独立。

Objective To investigate the relationship between the infection of high risk-human papillomavirues and lung cancer in north of Henan province,and preliminarily definitize the role of high risk-HPVs in carcinogenesis of lung cancer.

采用聚合酶链反应方法检测肺癌组织中高危型HPVs的感染情况及分型，并分析相关高危型HPVs感染与肺癌组织学类型的关系。

objective to analyze the drug resistance of staphylococcus aureusand provide suggestion for clinical rational use of antibiotics.methods secretion of those neonates suffered primary skin soft tissue infection and admitted during june,2005 to december,2007 was cultured and 113 strains of staphylococcus aureus were detected.we used k-b diffusion methods to determine their susceptibility to the common used antibiotics.results resistance rates of staphylococcus aureus to ampicillin/sulbactam,penicillin-g and cefazolin were 14.2%,40.7%and 6.2% respectively; there was no vancomycin-resistant strain.conclusion the resistance of staphylococcus aureus is not a serious problem in neonatal dermatic soft tissue infection.local treatment is the major approach in clinic,while general anti-infection consisting mainly of penicillin and first generation cephalosporin is chosen to avoid resistance mutation of staphylococcus aureus resulted from abuse of antibiotics.

多重聚合酶链反应快速鉴别葡萄球菌及。。。乳腺脓肿分离到葡萄球菌的鉴定和药敏。。。目的探讨金黄色葡萄球菌的耐药性，指导临床合理用药。方法对我院2005年6月至2007年12月新生儿原发性皮肤软组织感染患儿的分泌物进行培养，共检出金黄色葡萄球菌113株，并采用kb纸片扩散法对其进行常用抗生素的药敏试验。结果金黄色葡萄球菌对氨苄西林/舒巴坦、青霉素g及头孢唑啉的耐药率分别为14.2%、40.7%和6.2%，未发现耐万古霉素金黄色葡萄球菌。结论在新生儿皮肤软组织感染中金黄色葡萄球菌的耐药问题并不十分严重，临床处理应以局部为主，全身抗感染治疗宜以青霉素及i代头孢类抗生素为主，避免过度使用抗生素加速金葡菌的耐药突变。

Dichloroacetic acid ; ras gene ; DNA damage ; Randomized terminal linker-dependent PCR

二氯乙酸； ras基因； DNA损伤；依赖随机化末端连接物聚合酶链式反应

The concentrations of Mg^2＋, dNTPs, Taq DNA olymerase, primers which affect the SRAP-PCR reactions were optimized in order to establish the SRAP molecular marker system in Diospyros spp.

为了建立柿属植物SRAP技术体系，对影响SRAP-PCR的Mg^2＋、dNTPs、Taq聚合酶、引物浓度等因素进行了优化。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

Methods Eight coding exons of ED1 gene of two patients with clinically confirmed X-linked hypohidrotic ectodermal dysplasia, their parents, and 100 unrelated population-matched control were amplified by polymerase chain reaction. The products were further analyzed by direct sequencing.

采用聚合酶链反应扩增该家系中两名临床诊断为X性连锁少汗性外胚叶发育不良的患者、患者父母以及100例健康对照者ED1基因的8个外显子，进行DNA直接测序。

Objective: To evaluate the nested polymerase chain reaction technique in diagnosing endobronchial tuberculosis.

目的 ：了解巢式聚合酶链反应技术对支气管内膜结核的诊断价值。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。