聚合酶

Methods The antibody of Borrelia burgdoferi in the crowd serum was detected by immunofluorescence, and the spirochete DNA was tested with PCR.

人群血清伯氏疏螺旋体抗体的检测采用免疫荧光法，以聚合酶链反应检测伯氏疏螺旋体DNA。

In the RAPD system of Bryaceae the optimal concentration of template DNA was 2ng/μl, primer 0.4μmol/L, dNTP 0.2mmol/L, Mg2+ 2mmol/L, Taq DNA polymerase 1.25U, total volum 25μl. Several DNA samples were selected to choose the compatible primers.

通过单因素和正交试验对影响RAPD的因素进行考查，建立了优化的RAPD反应体系：dNTP为0.2mmol/L，随机引物0.4μmol/L，Taq DNA聚合酶1.25U，Mg2+为2mmol/L，模板DNA为50ng，扩增体系为25μl。

Methods The primer design is referring to thearticleof Bulter. Over 120 samples provided by the laboratoryare detected to verify the concordant results between miniSTR andcommon STR. We construct the miniSTR multiplex system usingdomestic Taq DNA polymerase, and detect the results by ABI—310Genetic Analyzer. We have alsostudied the sensitivity, accuracy, species-specificity and different PCR outcome under each annealtemperature.

方法参照Bulter等对THO1、TPOX、CSF1PO三个基因座miniSTR的引物设计，选用本教研室提供的123例无血缘关系个体的血样，进行THO1、TPOX、CSF1PO三个基因座PCR-miniSTR银染检测，检测结果与商品化试剂盒的检测结果进行一致性检验；采用国产DNA聚合酶构建miniSTR荧光标记复合扩增体系，用美国ABI-310基因分析仪进行检测；对该荧光标记复合扩增体系的灵敏度、准确性、种属特异性、不同退火温度下复合扩增的效果、陈旧血痕DNA的检测等进行研究。

The result showed that we have established a ITS-PCR system most suitable for Buxus sinica var. parvifolia, which came out total volume 50μL reaction system, was as follows: 2.0mmol/L Mg(superscript 2+), 0.3μmol/L primer, 0.3mmol/L dNTP, 240 ng/50μL DNA template, 1.75 U/50μL TaqDNA polymerase and the anneal temperature with 56℃.This optimized ITS-PCR system might ensure the purity and quality of ITS-PCR production. In this study, the length of the ITS fragment of Buxus sinica var.

实验结果表明，在总体积50μL的反应体系中，建立了最佳ITS-PCR扩增条件：Mg(上标 2+)浓度2.0mmol/L、引物浓度0.3μmol/L、dNTP浓度0.3mmol/L、DNA模板浓度240ng/50μL、TaqDNA聚合酶的用量1.75U/50μL和退火温度56℃，该优化体系保证了珍珠黄杨ITS-PCR产物的纯度和质量要求。

In this research, we extracted the genomic DNA from the Buxus sinica var. parvifolia leaves by using the modified CTAB approach and then used as the template DNA. Base on the invariable quantity of TaqDNA, we att empted to establish and optimize ITS-PCR amplification system for Buxus sinica var. parvifolia by employing L9 (3^4) orthogonal design with four factors, i.e. template DNA, Mg(superscript 2+), dNTPs and primer.

本研究利用改良CTAB法从珍珠黄杨叶片中提取基因组DNA作为模板，在TaqDNA聚合酶量不变的基础上，利用正交设计L9(3^4)对4个因素（模板DNA、Mg(上标2+)、dNTP和引物）在3个水平上对珍珠黄杨ITS-PCR反应体系进行优化。

The purpose of the work is to investigate the frequencies and polymorphisms of HIV-1 resistant CCR5delta32,CCR2b-64I,SDF1-3′A alleles in Chinese Dai and Chingpaw populations.Whole blood samples from 101 Dai subjects and 113 Chingpaw were collected randomly and their genomic DNA were extracted with QIAgen Blood Kits.Allelic frequencies were identified by PCR-RFLP analysis.Allelic polymorphisms in Dai population or Chingpaw population and both sexes in the samples were analyzed by χ2 test.

为了调查HIV-1感染相关的等位基因CCR5△32、CCR2b-64I、SDF1-3′A在我国云南省德宏州傣族景颇族人群中的频率和多态性分布，此课题以101例傣族和113例景颇族人群为研究对象，应用PCR、PCR-RFLP(聚合酶链反应-限制性片段长度多态性)分析方法进行检测，计算突变基因频率；并对其群体分布、性别分布进行统计学分析。

The present study was designed to elucidate the role of apoE polymorphism in the lithogenesis of cholecystolithiasis and to explore the hereditary pathogenesis of the disease. Polymerase Chain Reaction was used as researching apoE phenotypes and allele frequencies in patients with gallstones (n=87) and in controls (n=50), and the fasting serum lipids of subjects were also measured.

为从分子遗传学水平探讨胆囊结石病的发病机理，采用聚合酶链反应等方法研究了87例胆囊结石患者和50例非结石者的apoE基因表型及等位基因频率，并分析了不同apoE基因表型的胆囊结石患者的血脂质代谢特征。

The interaction of colloidal gold with Taq DNA polymerase was investigated in this study.

本文研究了胶体金与Taq DNA聚合酶的相互作用。

Objectives The aim of this study is to establish rapid methods for detecting group A streptococcus, corynebacterium diphtheria and Legionella pneumophila Lp Respectively by polymerase chain reaction

目的：建立用于检测A组链球菌、白喉杆菌以及嗜肺军团菌的多聚合酶链反应方法。

Methods The authors studied the expression of CyP gene from white blood cells in children with nephrotic syndrome by reverse transcription polymerase chain reaction.

采用逆转录聚合酶链反应测定28例肾病综合征患儿血白细胞中CyP mRNA的表达，并以正常儿童作对照。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。