聚合酶

Anaphylactoid purpura ; HLA ; polymerase chain reaction

过敏性紫癜；白细胞抗原；聚合酶链反应

Anaphylactoid purpura ; children ; Hans ; HLA-DQA1 gene ; polymerase chain reaction-sequence specific primer

过敏性紫癜；儿童；汉族；人类白细胞抗原DQA1基因；聚合酶链反应-序列特异性引物

Rats in LPS group (n = 12) were intratrachealy administered 200 μg of LPS dissolved in 100 μl saline after anesthetization, rats in control group(n = 12 ) were given intratracheal 100 μl saline.

通过检测LPS组大鼠气管注入LPS后第2、4、7天肺组织炎症指标包括肺炎症积分、支气管肺泡灌洗液中性粒细胞计数、巨噬细胞计数、TNF-α、IL-1β及IL-8含量和气道黏液分泌指标包括肺组织阿先蓝—过碘酸雪夫阳染面积、免疫组化黏蛋白Muc5ac及逆转录-聚合酶链反应Muc5ac mRNA的表达，对LPS诱发大鼠肺炎症和气道黏液高分泌的改变进行动态观察。

Litter size, sex ratio at birth, and anogenital distance in offspring of both sexes were examined, as well as vaginal opening in female offspring.

采用逆转录聚合酶链反应技术和免疫组织化学染色技术检测各组胎盘ERα、ERβ受体的表达。

The expression of CSE1L gene was identified using these aRNA by sqRT-PCR.

结合RNA保护、显微切割及线性扩增技术，获得纯化鼻咽组织中反义RNA（antisense RNA，aRNA），采用半定量逆转录聚合酶链式反应（semi-quantitative reverse transcriptionpolymerase chain reaction，sqRT-PCR）研究从全基因组表达谱中筛选的候选癌基因-CSE1L在两种不同纯化鼻咽组织aRNA中的表达。

Objectives To search for mutations in NOTCH3 gene in four Chinese families with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy.

方法对 4个经临床和病理检查证实的CADASIL家系中的先证者作NOTCH3基因编码区外显子 1～ 12的聚合酶链反应和DNA测序，对家系 2和 4中的部分亲属也作了同样的检查。

The RNA polymerase binding site is adjacent to OR2. This explains how repressor autogenously regulates its own synthesis.

它们排在抑制子的一个位点上，该位点与DNA 磷酸基因邻近，而这个磷酸基因也与RNA聚合酶邻近。

Objectives: The purpose of this study is to analyse the point mutation of DYS1 in azoospermic patient and to find the relationship between point mutation and azoospermia.

用聚合酶链反应方法检测了染色体核型正常的200例无精子症患者基因组DNA中的DYS1基因，发现8例有DYS1的缺失。

Objective To identity bacteria of bacteroides group by PCR.

目的 用聚合酶链反应技术鉴定拟杆菌。

After treatment of benzamide which is an inhibitor of poly polymerase,β-galactosidase activity assays and PCR analysis were performed to test the deletion of LacZ gene and c-Ha-T24ras gene. The results showed that:(1)LacZ gene was deleted from both A13.4-NIH3T3 cells and B12.7-NIH3T3 cells, but neigher from A13.4-HeLa cells nor B12.7-HeLa cells. So it is possible that there is cell line specificity in gene deletion induced by BA.(2) In A13.4-NIH3T3 cells, LacZ gene and c-Ha-T24ras gene were completely deleted at the same time.(3) The transcription activity of exgenous DNA fragment may have same effect on its sensitivity to the deletion induced by BA.

用聚ADP核糖聚合酶的NAD位点抑制剂苯甲酰胺分别处理四种转化细胞后，检测细胞中整合的外源LacZ基因与c-Ha-T24ras基因的删除情况，结果如下：BA诱导的基因删除可发生于NIH3T3转化细胞系，但不能发生于HeLa转化细胞系；位于同一外源表达载体上的LacZ基因与c-Ha-T24ras基因的删除过程是同步的；外源整合DNA片段的转录强度可能直接影响其被BA诱导删除的敏感性。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。