聚合酶

Kary Mullis of Human Heredity Department, the Cetus Company set up this technology by using Klenow DNA polymerase .

聚合酶链式反应是一种模拟DNA 聚合酶在体内的催化作用，在体外快速扩增特定DNA片段的技术，是现代分子生物学最重要的手段之一。

In this thesis, we studied the effects of nuclear actin on gene transcription regulation, with a special focus on the relations between the nuclear actin and RNA polymerase II in concanavalin A-stimulated lymphocytes and HeLa cells.

本文以ConA激活的淋巴细胞和HeLa细胞为实验材料，从不同角度研究了细胞核肌动蛋白与RNA聚合酶Ⅱ相互作用，并对细胞核肌动蛋白参与RNA聚合酶Ⅱ的作用机制进行了探讨。

To date, three kinds of reverse genetics systems, in vitro transcription system, system induced by endogeny T7 RNA polymerase and system induced by RNA polymeraseⅡ, were used to research IBDV. But these systems have some shortcomings in the efficiency and convenience of virus rescue.

目前用于IBDV研究的体外转录、基于T7RNA聚合酶的体内转录、RNA聚合酶Ⅱ体内转录等反向遗传操作系统在病毒拯救效率和操作简便性等方面仍然存在着一些问题。

In recent years, more and more experiments indicated that RNA polymerase Ⅲ transcription might not occur in the nucleoplasm but in the nucleolus. Nevertheless, there is no direct experimental proof. In this dissertation, the plasmids bearing pol Ⅲ recognized promoters were transfected into the CHO cells by electroporation.

由于RNA聚合酶Ⅲ转录位点的研究存在特殊性，而传统的研究方法又存在一定的局限性，因此近几年人们对RNA聚合酶Ⅲ的转录发生在核质中这一结论提出了质疑，但至今尚缺乏直接的实验证据。

The transcription localizations of these polymerases have been studied since early 1960s. The results of these early research works showed that the transcription of RNA polymerase I was localized in the nucleoli while that of RNA polymerase Ⅲ in the nucleoplasm.

自上个世纪六十年代初期，人们相继运用细胞化学染色、电镜放射自显影等进行研究的结果表明：RNA聚合酶Ⅰ的转录发生在核仁中，RNA聚合酶Ⅲ的转录发生在核质中。

Recombinant adenovirus was identified by polymerase chain reColon cancer cell line SW480 was infected with recombinant adenovirus Ad-p27mt, and expression of p27 protein was detected by Western blot.of expressed p27 protein after Ad-p27mt transfected colon cancer cell.novirus framework plasmid pAdeasy-1 with pShuttle-CMV-hp27mt, 30％combinant adenovirus DNA contained the target gene.

Ad-p27mt的聚合酶链反应检测Ad-p27mt转染大肠癌细胞后的p27mt的表达。结果：①用含pShuttle-CMV-hp27mt转化含pAdeasy-1的超感受态BJ5183后，可获得约30％的阳性重组质粒。②聚合酶链反应检测表明重组腺病毒DNA中含有目的基因。重组腺病毒滴度为7.95×1015pfu/L。

E.coli RNA polymerase can transcribe all the genes of E.coli, but the three eukaryotic RNA polymerases transcribe only specific, nonoverlapping subsets of eukaryotic genes.

eukaryotic genes 大肠杆菌的RNA聚合酶能够转录所有的基因，但真核生物的三种RNA聚合酶各自只能转录特定的、不相重叠的一组基因。

However, with the development and the application of new techniques, the reliability of the methods used in the research of the transcription site of RNA polymerase I was doubted; and also, some indirect results indicated that the transcription of RNA polymerase Ⅲ might occur in the nucleoli.

但随着新的研究技术的发展和应用，人们对RNA聚合酶Ⅰ转录位点的早期的研究方法的可靠性提出质疑；同时，根据一系列间接的实验推论RIgA聚合酶Ⅲ的转录可能发生在核仁中。

A new picorna-like virus was isolated from dead Evtropis oblique larvae deceased from NPV infection. Electron microscopic observations of purified virions were non-enveloped isometric particles with diameter of 26nm. The virions contain two capsid proteins: 31.5kDa and 28.8kDa, the amount of later is 2.5 times more than that of the former. Analysis of 3′terminal sequence of EoPV clone identified that it can encode RdRp and has eight conserved motifs. Homology analysis shows that it is closely related to Perina nuda picorna-like virus.

从核型多角体病毒感染致死的茶尺蠖幼虫尸体中分离到一株微小RNA病毒透射电镜观察纯化的病毒粒子为无囊膜、无表面特征、直径约26nm的球状颗粒。16%的SDS-PAGE显示它有两个分子量为31.5 kDa和28.8 kDa的衣壳蛋白，后者的含量是前者的2.5倍。3′端克隆序列分析表明EoPV基因组3′有poly尾，编码RNA聚合酶，含有微小RNA病毒RNA聚合酶的八个保守基序，同源性分析表明它与榕透翅毒蛾微小RNA病毒亲缘关系最近。

Methods K-ras gene point mutation and its style at codon 12 of Patu 8988 cell were detected by using PCR-SSP and sequence analysis.

方法顺序特异引物聚合酶链反应法和基因测序检测Patu8988细胞K-ras点突变形式，根据点突变形式设计并合成硫代反义寡核苷酸（K-ras mutation ASODN）作用Patu 8988，通过噻唑蓝比色法检测细胞生长情况；流式细胞术检测K-ras蛋白表达和细胞凋亡；逆转录-聚合酶链反应检测K-ras mRNA表达水平；以Patu8988细胞建立裸鼠胰腺癌模型观察K-ras mutation ASODN在体内的抗肿瘤效果。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。