聚合酶

Objective To evaluate the therapeutic effect of azithromycin on infant toxoplasmosis patients by using fluorescence quantitative polymerase chain reaction.

目的探讨荧光定量聚合酶链反应用于评价阿奇霉素治疗儿童弓形虫病的效果。

Methods:15 cases of conjuctivitis were detected for the trachoma chlamydia by fluoroqenic quantitative PCR and super microscope diagnosis system.

利用荧光定量聚合酶链反应和超高倍显微诊断系统镜检检测 15例结膜炎患者的沙眼衣原体。

Methods The expression plasmid pSVvWF containing full-length cDNA of vWF was used to site direct mutagenesis by polymerase chain reaction and transitorily expressed in the COS-7 cells, vWF:Ag in the supernatant and the cell lysate between wild type and pSVAla737Glu vWF were measured.

用聚合酶链反应的方法对野生型的vWF全长表达质粒进行体外定点诱变，在COS-7细胞中进行表达。

These results indicate that treacle, but neither UBF nor Nopp140, is essential for nucleolar recruitment of Pol I transcription complex.

这些结果显示，treacle，而非UBF或Nopp140，在核醣核酸第一型聚合酶复合体聚集至核仁的作用中扮演重要角色。

ObjectiveTo assess the application value of fluorescence quantitative polymerase chain reaction for detection of treponema pallidum DNA.

目的：评估荧光定量聚合酶链反应技术检测梅毒螺旋体DNA的应用价值。

In order to establish a quick and reliable identification system for the main Citrus viruses, total RNA was isolated from the sampled trees showing typical symptoms of Citrus tristeza virus, Citrus exocortis viroid and Citrus tatter leaf virus for RT-PCR analysis.

为建立主要柑橘病毒病准确、快速的分子检测体系，在表现柑橘衰退病、柑橘裂皮病和柑橘碎叶病典型症状的病树上采叶样提取总RNA进行反转录聚合酶链式反应分析。

Reverse Transcription-Polymerase Chain Reaction was used to clone the whole-length gene of BMP-2 and construct AdV vector.

应用反转录-聚合酶链反应法克隆出骨形态发生蛋白质类2全长基因，构建重组腺病毒载体，DNA-磷酸钙共沉法将辅助质粒PJM17转染293细胞，同源重组构建出重组腺病毒。

Hen THP-1 macrophages were induced to the foam cells by ox-LDL. Treatment of THP-1 macrophage derived foam cells with apoA-Ⅰ, forskolin (FRK, an adenyl cyclase activator), and SQ-22536 for long periods of time (24 h). In addition, THP-1 macrophage derived foam cells were treated with increasing amounts of FRK (0, 10, 20, 40 and 80 μmol/L) and treated with FRK for increasing time(0, 6, 12 and 24 h).

HP-1巨噬细胞源性泡沫细胞经各种因素处理后，采用油红&O&染色，观察细胞内的脂滴，运用液体闪烁计数器检测细胞内胆固醇流出，高效液相色谱分析细胞内总胆固醇、游离胆固醇和胆固醇酯含量，用逆转录-聚合酶链反应和蛋白质印迹分析法分别检测ABCA1 mRNA与ABCA1蛋白质的水平。

Adult male Wistar rats were randomLy allocated to 3 groups:control group, stone-forming group, Alisma Orientalis group. Reverse transcription polymerase chain reaction technique was used to examine bikunin mRNA expression levels in rat renal tissue.

将Wistar大鼠随机分成对照组、结石模型组、泽泻组，采用半定量逆转录聚合酶链反应检测大鼠肾组织bikunin mRNA的表达水平、镜下观察肾组织草酸钙晶体分布，同时检测大鼠血生化、肾钙含量、24h尿钙和尿草酸的分泌量。

Through a combination of T7 RNA polymerase and T7 Promoter for testing proper buffer was selected.The experimental results showed 5×binding-Buffer 1 was more suitable for the test;TBE running-buffer was better than TAE and TGE buffer;0.2×TBE buffer was the best concentration amang there electrophoresis buffer.

通过用T7RNA聚合酶与T7启动子DNA的结合反应来选择合适的缓冲液，结果表明，不同的缓冲液会影响凝胶迁移试验的结果，5×Buffer1结合缓冲液的效果较好；TBE电泳缓冲液比TAE、TGE的电泳效果好，同时在3种不同浓度的TBE缓冲液中，0.2×的TBE效果最好。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。