聚合酶

Methods: Real time FQ - PCR testing and germiculture methods adopted for NG,CT,UU testing on spermatic fluids of 105 cases of male infertility and regular spermatic fluid testing.

采用实时荧光定量聚合酶链反应技术对105例男性不育者的精液进行NG、CT、UU检测并做精液常规检查。

Methods The antibody of Borrelia burgdoferi in the crowd serum was detected by immunofluorescence, and the spirochete DNA was tested with PCR.

情况。方法人群血清伯氏疏螺旋体抗体的检测采用免疫荧光法，以聚合酶链反应检测伯氏疏螺旋体DNA。

A 3-1E gene fragment was amplified from Eimeria acervulina SH strain sporozoite by RT-PCR,and it was cloned into eukaryotic expression vector pcDNA3.1 to construct the recombinant plasmid pcDNA3.1-3-1E.

应用反转录-聚合酶链式反应技术从堆形艾美球虫SH株子孢子中扩增3-1E基因片段，将其克隆入真核表达载体pcDNA3.1中，构建重组表达质粒pcDNA3.1-3-1E。

In this study, the cDNA fragments of G-L intergenic region and polymerase activity module in L gene of two street rabies virus were cloned and sequenced. The nucleotides sequence of these cDNA fragments mentioned above and their deduced amino acid sequence were compared with that of rabies virus strains, rabies-related virus strains and other Rliabdoviridae published previously. The result show that the homology of nucleotide sequence of G-L IGR among MRV, DRV is 83.3%, compared with other rabies-related virus strains, the homology of G-L IGR is between 58.3%-91.7%. the length of G-L IGR of rabies virus is different in Rhabdoviridae.

本实验对狂犬病病毒野毒株MRV、DRV的G-L间隔区以及L基因聚合酶活性部位进行了克隆和测序，将所测定的结果和推定的氨基酸序列与国内外公开发表的狂犬病病毒其它固定毒株、野毒株和同科不同属的病毒的相应部分进行了分析比较，结果表明MRV、DRV G-L IGR核苷酸序列的同源性为83.3％，与其它毒株的同源性在58.3％～91.7％之间；狂犬病毒G-L间隔区序列的长度在弹状病毒科不同病毒属之间有所不同。

45W related genes were amplified by RT-PCR from the hatched and activated oncospheres of Taenia solium, cloned and sequenced.

利用反转录聚合酶链式反应从激活的猪带绦虫六钩蚴克隆到45W基因家族。

45W related genes were cloned from the activated oncosphere of Taenia solium.

利用反转录聚合酶链式反应从激活的猪带绦虫六钩蚴克隆到45W基因家族。

DNA fingerprinting for Taraxacum mongolicum and its adulterants of six species of Compositae was demonstrated with random primed polymerase chain reaction including arbitrarily primed PCR and random amplified polymorphic DNA.

采用分子生物技术包括任意引物聚合酶链式反应和随意引物扩增的多态性DNA方法扩增蒲公英及其6种土公英混淆品的基因组DNA，获得清晰可靠的DNA指纹图谱。

Chinese drug ; identification ; Taraxacum mongolicum ; polymerase chain reaction ; DNA fingerprinting

药材鉴定；蒲公英；聚合酶链式反应； DNA指纹图谱

The PCR products respectively from a donor (TX2), a chronic hepatitis B patient (TX3), a chronic hepatitis C patient (TX4) and a hepatitis patient with unknown etiology (TX1) were sequenced by the dideoxy chain terminating method.

以套式聚合酶链反应测定TT病毒核酸，取献血员(TX2)、慢性乙型肝炎(TX3)、慢性丙型肝炎(TX4)及慢性非甲～戊型，非庚型肝炎患者(TX1)各1例TT病毒PCR阳性产物，以双脱氧核苷酸链终止法测定核苷酸序列。

MAIN OUTCOME MEASURES: Evaluation of PCR and DNA sequencing were used for confirming the size of segment and correctness of rank of MyoD cDNA and detecting the titre of virus.

主要观察指标：聚合酶链反应鉴定和DNA测序来证实MyoD cDNA片段大小和序列的正确性，并测定病毒滴度。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。