聚合酶

With western blot analysis and DNA polymerase chain reaction-single strand conformation polymorphism assay,protein product of Rb gene and mutation in exon 5～8 of P_(53) gene were examined in 17 cases of human testicular seminoma.

采用Western印迹和DNA聚合酶链反应－单股构造多态性分析法对17例人睾丸精原细胞瘤组织标本的抗癌基因Rb表达的蛋白产物和P_（53）基因外显子5～8的突变进行检测，发现有3例标本的Rb基因表达的蛋白产物缺失，有4例标本的P_（53）基因有点突变，对照的正常人睾丸组织和视网膜组织均呈Rb基因蛋白产物表达阳性，对照的正常人睾丸组织中未发现P_（53）基因突变。

Then we used the semiquantitative PR-PCR to verify the result.

并采用半定量逆转录聚合酶链反应验证结果。

Methods The expression levels of TNF-α and IL-1α mRNA by PBMCs were assessed by semiquantitative RT-PCR.

TNF-α和IL-1α mRNA的表达水平采用半定量逆转录-聚合酶链反应进行检测。

Methods: Expression of TrkA, TrkB and VEGF mRNA was semiquantitatively detected by reverse transcription-polymerase chain reaction in 51 cases of neuroblastomas.

采用逆转录聚合酶链反应法半定量检测51例NB肿瘤中TrkA ，TrkB及VEGF mRNA的表达。

METHODS: Reverse transcriptasepolymerase chain reaction was applied to semiquantitatively analyze CXCR3 mRNA expression in peripheral blood lymphocytes and neutrophils from 33 psoriatic cases, 16 cases with about 70% remission of the 33 patients after treatment and 30 healthy controls.

应用逆转录聚合酶链反应法检测进行期斑块型银屑病患者33例及其中16例患者治疗后外周血淋巴细胞及中性粒细胞中CXCR3 mRNA的表达水平，设30例健康人作为正常对照，并将检测结果与PASI进行了相关性分析。

The expression of NOSⅢ mRNA was detected semiquantitatively by reverse transcription-polymerase chain reaction.③Immun ocytochemistry was used to detect the expression of NOSⅢ protein.

将原代培养的CAEC进行缺氧1 h，复氧2、6、12和24 h，用逆转录-聚合酶链式反应和免疫细胞化学方法检测NOSⅢmRN A和蛋白质表达的变化。

In order to establish a sensitive immuno-PCR assay for detecting serai anti-P53antibodies, investigate the relation of anti-P53 antibodies in patients with breast carcinoma to P53 protein expressions in tissue and clinical significances and afford a practical tool in diagnosis of breast carcinoma clinically. SaHIgG was coupled to PNCa DNA by chemical cross-linker PEI and SaHIgG-DNA probe was constructed.

本研究建立了血清抗P53蛋白抗体免疫聚合酶链反应(Immuno-polymerase chain reaction，免疫PCR)检测方法，探讨了乳腺癌患者血清中抗P53蛋白抗体与组织中P53蛋白表达之间的关系及临床意义，旨在为临床乳腺癌P53蛋白检测提供实用的替代工具。

Methods The template DNA was extract from the human osteosarcoma cells line U－2OS by the single- step isolation method with isothiocyanic acid guanidine, the cDNA coding for the mature fragment of BMP－4 was amplified by the reverse transcription- polymerase chain reaction.

方法采用异硫氰酸胍一步法从人骨肉瘤细胞株U-2OS中提取总RNA，通过逆转录-聚合酶链反应扩增为BMP-4成熟区cDNA基因片段。

Methods After L-02 cells were incubated with 40, 80, and 160μg/ml sodium fluoride for 24 hours, the cell survival rate, the level of P53 protein and mRNA expression was assessed by MTT, Western blot and RT-PCR analysis respectively. The cell survival rate in L-02 cells treated by high concentration of fluoride was significantly lower than that in the control group.

方法］对体外培养的人胚肝细胞染毒氟化钠40、80、160μg/ml 24h后，分别采用MTT试验、蛋白印迹技术和逆转录－聚合酶链式反应方法检测人胚肝细胞存活率、P53蛋白和mRNA水平。

Objective To evaluate specimen processing with sodium dodecyl sulfate on the detection of Mycobacterium tuberculosis by PCR-reversed dot hybridization.

目的探讨应用十二烷基硫酸钠处理标本在结核分支杆菌聚合酶链反应中的价值。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。