聚合酶

Spotted fever group rickettsia\ \ Polymerase chain reaction

斑点热群立克次体；聚合酶链反应

Methods: The experimental g roup consisted of 24 patients with PE and 24 cases of normal placentae women respectively. Group of 24 patients with PE consisted of 12 mild cases and 12 severe cases. RT-PCR and Western Blotting were used to test the difference content of HLA-G between two groups.

采用半定量逆转录-聚合酶链技术及Western印迹技术分别检测了24例子痫前期患者(实验组，轻度子痫前期12例，重度子痫前期12例)，24例年龄相仿的正常晚期妊娠者胎盘组织中HLA-G的表达差异。

Bioinformatics analyses and nuclear run-on transcription assays indicate that Pol IV does not engage in the efficient RNA synthesis typical of the three major eukaryotic DNA-dependent RNA polymerases.

生物信息学分析和核的连续转录分析表明，Pol IV并不参与三大真核细胞DNA依赖性RNA聚合酶的高效RNA合成。

Methods Six recombinant plasmids with shRNAs targeting the PDE5A3 gene of Homo sapien were constructed.

方法构建6个靶向人PDEsA3基因的短发夹RNA重组质粒，转染人阴茎海绵体平滑肌细胞48 h后，逆转录-聚合酶链反应及Western blot检测PDE5A3基因的表达抑制效果。

Polymerase chain reaction was performed on lesion scrapings, aspirates, and filter paper impressions.

对病灶进行刮除，抽吸和滤纸印迹并进行聚合酶链反应。

Methods The nested polymerase chain reaction technique was used to detect the blood samples of patients with scrub typhus and compared with serologic detection of Rt.

采用巢式聚合酶链反应对11例确诊及10例疑诊恙虫病患者血液标本进行检测，并以血清IgG抗体间接免疫荧光试验对比分析。

Aim: To set up the methods of polymerase chain reaction-sequence specific primers for human ABO blood group, HAB secretor status and MN blood group genotyping and study the polymorphism of ABO blood group genes, HAB secretor status genes in part of Han population in Chinese and MN blood group genes in random blood donors and study the application of ABO genotyping in clinical transfusion and genetics.

目的：采用聚合酶链反应-序列特异性引物扩增基因定型方法，对ABO血型、HAB分泌型和MN血型系统基因定型，研究ABO血型、HAB分泌型和MN血型基因多态性分布特征及ABO血型基因定型在临床输血、遗传分析中的应用。

Upon initiation of PCR after the denaturation phase, the single-stranded DNA fragments flanked by inverted terminal repeats may form either self-annealing "pan-like" structures (preventing the primer binding to its complementary binding sites and suppressing the PCR) or DNA/primer hybrid structures.

经聚合酶链反应开始后，变性阶段，单链 DNA片段两侧末端反向重复序列可能两种自我退火"泛，如"结构（防止引物结合到其互补性结合位点和制止的PCR ）或DNA /引物混合结构。

Objective To design and establish a new method to dectect patho-genic bacterial 16S rRNA gene in cerebrospinal fluid using mutiplex semi nested PCR.

目的 建立多重半套式聚合酶链反应（multiplex semi-nested PCR）快速检测脑脊液标本中常见病原菌的方法。

Semiconservative replication :The two strands of DNA double helix are separated, each strand serves as a template for the synthesis of a new complementary strand, producing two daughter molecules. Each daughter molecule has one parental strand and one newly synthesized strand.

半保留复制： DNA 生物合成时，母链 DNA 解开为两股单链，每股单链分别作为模板，以 dNTP(dATP 、 dGTP 、 dCTP 、 dTTP)为原料，按照碱基配对(A － T 、 G － C)规律与模板上的碱基配对，经依赖 DNA 的 DNA 聚合酶催化，合成一条与模板互补的新链，新形成的两个子代 DNA 与亲代 DNA 碱基序列一致，子代 DNA 分子中一股单链是从亲代完整地接受过来，另一股单链是新合成的，故称为半保留复制。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。