聚合酶

Methods The factor Ⅴ Leiden mutation was analyzed in 75 healthy and 118 ICVD patients using PCR-RFLP analysis.

应用聚合酶链反应和限制性片段长度多态性分析方法对国人 118例ICVD患者及 75名健康者进行FⅤLeiden突变检测，86例ICVD患者检测aCL ，2 7例进行抗活化蛋白C检测。

Methods MHC-DRB1, DQA1, DQB1 were examined by using polymerase chain reaction/sequence specific oligonuleotide probe methods in 19 patients with miliary tuberculosis, 36 with tuberculous menigitis,20 with primary tuberculosis, 28 with tuberculous pleurisy and 91 controls.

采用聚合酶链式反应-序列特异性寡核苷酸探针斑点杂交方法，测定原发性肺结核20例、结核性胸膜炎28例、粟粒型肺结核19例、结核性脑膜炎36例和91例正常对照组的MHC-DRB1，DQA1，DQB1等位基因。

The I/D polymorphism of ACE was detected by polymerase chain reaction, and the CMA/B poly- morphism of Chymase gene was detected using PCR-restriction fragment length polymorphism.

运用聚合酶链反应检测 ACE基因的I/D多态性，运用限制性片段长度多态性技术检测Chymase基因的CMA/B多态性。

Key words: Muscovy duck parvovirus;goose parvovirus;PCR;differential diagnosis

番鸭细小病毒；鹅细小病毒；聚合酶链式反应；鉴别诊断

Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples.

聚合酶链反应和DNA探针联合检测临床标本中结核杆菌的研究。

Methods:Control trial was adopted in the experiment design.Immunochemistry and RT-PCR were used to detect the expression of MMP-7 in nasopharyngeal carcinoma and chronic nasopharyngitis.

采用成组设计的对照试验方法，运用免疫组化及逆转录聚合酶链式反应技术，检测MMP-7蛋白及mRNA在鼻咽癌及慢性鼻咽炎组织中的表达。

Methods Specimens of neurofibroma tissue, keloid and normal skin were collected. The distribution and expression of CTGF was measured by immunohistochemistry. The mRNA expression of CTGF, TGF-β1,ColⅠ and ColⅢ was detected by reverse transcription- polymerase chain reaction. Expression of CTGF protein was measured by western blotting.

收集神经纤维瘤组织、瘢痕疙瘩组织及正常皮肤组织标本，免疫组化检测CTGF的表达，逆转录-聚合酶链式反应方法检测组织CTGF、TGF-β1、Ⅰ型胶原及Ⅲ型胶原 mRNA表达，Western blotting检测组织CTGF蛋白的表达。

In this dissertation we present our results on the regulatory mechanism of expression of nodulation genes, mainly focusing on the bacterial RNA polymerase and transcriptional factors.

这些基因的表达受到了严格的调控。本文中我们从RNA聚合酶和转录调控因子两个方面研究了结瘤基因表达的调控机制。

Methods Real-time PCR, using the primer-probe specific for the yst gene, was applied to detect the pathogenic （7 serotypes） and nonpathogenic （ 5 serotypes） Yersinia enterocolitica, other species of Yersinia （8 types）, and other enteric bacteria （8 types）.

应用针对致病性小肠结肠炎耶尔森菌染色体上yst基因而设计的特异引物和探针进行实时定量聚合酶链反应，检测致病性（7种血清型）和非致病性（5种）小肠结肠炎耶尔森菌、耶尔森菌属内的其他菌种（8种）及其他肠道细菌（8种）。

A portable polymerase chain reaction system automates all the steps, including sample preparation, allowing operation by nonscientist users in the field and providing results in only 30 minutes.

一种便携式聚合酶链反应系统能够自动完成包括样品准备在内的所有实验步骤，从而使得不是该领域的非专业操作者也能够完成检测，而且只要30分钟就能够得到结果。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。