聚合酶

The total RNA was extracted from spleen of Xiphophorus helleri which had been immunized for two weeks. The core sequence was amplified by RT-PCR. The cDNA end sequences were amplified by RACE, and then the full-length cDNA of sIgM heavy chains gene were obtained by putting all of them. The full-length sequence contains the immunoglobulin signature sequence: FKCIANH.

以免疫两周的剑尾鱼为材料，提取脾脏总RNA，通过逆转录－聚合酶链式反应扩增出分泌型免疫球蛋白M(secretory immunoglobulin M， sIgM)重链基因的核心序列，再应用3'和5'快速扩增cDNA末端方法扩增其末端序列，拼接后获得剑尾鱼sIgM重链全长cDNA序列。

Measles virus ; S191; hemagglutinin gene ; reverse transcription polymerase chain reaction ; sequence analysis ; molecular epidemiology

麻疹病毒； S191； H基因；逆转录—聚合酶链反应；序列分析；分子流行病学

Nested reverse transcription polymerase chain reaction revealed that c-SPH mRNA is expressed most in tissues with the highest hemocyte abundance.

巢状反转录聚合酶连锁反应显示c-SPH mRNA的讯号在血球中最为强烈。

The further analysis was given to Rhesus boxes specific sequencing and RHD gene homozygosity determined by PCR-restriction fragment length polymorphism analysis to Rhesus boxes.

然后对Rh盒子基因进行特异性测序分析，同时对Rh盒子基因的扩增产物采用聚合酶链反应-限制性片段长度多态性（restrict fragment length polymorphism，RFLP）方法进行RHD基因的纯合性测定。

Preparation and collection of specimens: To kill the rabbits at different time and collect the specimens for pathology, electron microscope, RT-PCR and orthotopic hybridism in situ.

标本留取：分别于术后第2、4周处死假手术组大白兔各2只，于术后即刻随机处死对照组和通心络组大白兔各2只，损伤后3天、1周、2周、4周和6周随机处死对照组和通心络组大白兔各4只，分别留取病理、电镜、逆转录-聚合酶链式反应和原位杂交试验标本。

In this study, the new technique of PCR was investigated for its feasibility in the estimation of virus inactivation.

2探讨聚合酶链式反应用于评价病毒灭活效果的可行性，探索病毒灭活评价的新方法。

Methods:70 juvenile patients with AP and 90 normal children in Inner Mongolia region were tested for HLA-DQA1 allelic types analysis by PCR-SSP technique.

采用聚合酶链反应-序列特异性引物分型技术对 70例内蒙古地区的儿童AP病人进行了HLA -DQA1等位基因型别分析，并与 90例正常儿童进行比较。

This paper reviewed the advances in honeybee breeding involving to estimate the numbers of subfamilies in a colony and analyze the genetic correlation among workers; guess the polyandry level of queens and identify the purity of a subspecies; build a linkage map and discriminate loci of quantitative traits; genotype and distinguish full sister from semi sisters; analyze the trend of sperm to inseminate an egg and so on by means of such molecular biotech as microsatellites DNA, mitochondria...

综述了应用微卫星 DNA技术、线粒体 DNA技术、随机扩增多态性 DNA、聚合酶链式反应和 DNA指纹等分子生物技术所进行的蜜蜂育种研究现状。其应用主要包括估计蜂群内亚家系的组成数目和分析工蜂间的遗传相关，推断蜂王的多雄水平和鉴定蜜蜂种性，构建连锁图和区别数量性状位点，鉴定基因型和区别全胞、半胞姐妹以及分析精子受精力的倾向性等

Objective To expiore the pathogenic mechanism of hemorrhagic fever with renal syndrome.Methods 2-3 days old inbred Balb/C suckling mice were inoculated intracerebrally with tissue suspension of soft parate petechia of HFRS patients and the HFRS virus RNA was tested in brain, lung and kidney tissues of sucking mice by nested PCR.

目的 探讨肾综合征出血热的发病机理方法 2～3天的近交系Balb/C乳鼠，脑内接早期HFRS重症患者软腭粘膜出血点组织悬液，用套式聚合酶链式反应检测乳鼠脑、肺及肾组织中的HFRS病毒RNA。

Methods 2-3 days old inbred Balb/C suckling mice were inoculated intracerebrally with tissue suspension of soft parate petechia of HFRS patients and the HFRS virus RNA was tested in brain, lung and kidney tissues of sucking mice by nested PCR.

2～3天的近交系Balb/C乳鼠，脑内接种早期HFRS重症患者软腭粘膜出血点组织悬液，用套式聚合酶链式反应检测乳鼠脑、肺及肾组织中的HFRS病毒RNA。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐