聚合酶

Mrnas: these provide protein coding information to ribosome. some snrnas: these function in a variety of nuclear processes, including rna splicing and processing. it is very sensitive to inhibition by -amanitin.

rna聚合酶ⅱ催化合成 mrna的前体核内不均一rna以及核内小分子rna.mrnas是核糖体翻译蛋白质的模板；核内小分子rna参与了核内的多种加工过程，包括rna的剪切加工。rna聚合酶ⅱ对α-鹅膏蕈碱高度敏感。

Nucleolus 核仁 28s, 18s, 5.8s rrna: these are structural and functional components of the ribosome, which functions during translation. it is relatively insensitive to inhibition by -amanitin.

rna聚合酶ⅰ催化合成 rrna(5.8s、18s和28s rrna)的前体，5.8s、18s和28s rrna 是具有翻译功能的核糖体的结构和功能成分。rna聚合酶ⅰ对α-鹅膏蕈碱不敏感。

Trnas: these carry amino acids to the ribosome during translation. some small nuclear rnas: these function in a variety of nuclear processes. 5s rrna: this is a functional and structural component of the ribosome's 60s subunit. it is somewhat sensitive to inhibition by -amanitin.

rna聚合酶ⅲ催化合成5s rrna、trna的前体，以及核内小分子rna和病毒rna.trna在翻译过程中能够荷载氨基酸；核内小分子rna参与了核内的多种加工过程；5s rrna是60s大亚基的结构和功能成分。rna聚合酶ⅲ对α-鹅膏蕈碱中度度敏感，因生物的种类不同，敏感性不同。

The metabolism of these extreme microbes during the production of Maotai Liquor would further produce multiple enzymes of thermal stability such as amylase, protease, saccharifying enzyme, cellulose, glucase, xylanase, and each kind of dehydrase involved in redox reaction, and DNA polyase etc.

茅台酒酿造过程中极端酿酒微生物代谢产生多种热稳定性的酶，如淀粉酶、蛋白酶、糖化酶、纤维素酶、葡萄糖甘酶、木聚糖酶、参与氧化还原反应的各种脱氢酶、磷酸烯醇丙酮酸激酶及DNA聚合酶等。

A constantly fluctuating brigade of enzymatic foot soldiers reg ulates RNA polymerase II's activity by chemically modifying the long cord-li ke tail that hangs from its globular structure, like a chain on a light fixt ure.

有一组不断变动的酶通过化学修饰RNA聚合酶II球状结构长带状尾巴来调节RNA聚合酶II的活性，就像电灯组件后电线。

To compensate the descent of pH for the heat-up, exalting the Tris-HCl buffer's pH can avoid the abscission of the purine and make the long sequence PCR go on smoothly. The GC-rich template's secondary structure is complex and stable. The organic reagent methylamine contributes to uncoil the secondary structure.The pfu DNA polymerase has more fidelity and thermostability than Taq DNA polymerase.

高GC含量使模板二级结构复杂而稳定，一般热启动难于解开，而变性温度过高、时间过长又不利于DNA聚合酶的活性作用发挥，于是借助能缓释模板二级结构的有机试剂甲胺扩增高GC含量模板。pfu DNA聚合酶比Taq酶有更高的保真复制性能和热稳定性，更有利于高GC含量模板的扩增。

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段，再通过聚合酶链反应使MyoD基因加上CACC序列接头，经过定向拓扑克隆使目的基因连接到转移载体上，再通过LR酶促反应，将目的基因转移到腺病毒表达载体DNA上，获得MyoD基因重组的腺病毒DNA，用脂质体转染法转染HEK293A细胞，包装扩增出MyoD基因重组的腺病毒。

Methods The DNA chip was fabricated,on which oligonucleotide bougies′length was 19 base pairs,and the concentration was above 15 μmog/L.

方法自制DNA芯片，每条寡核苷酸探针片段长度为19bp，点样浓度在15μmol/L，以痰液为待检标本，分别用不同引物、Mg2+、核苷酸、DNA聚合酶浓度等进行多重聚合酶链反应；分别在不同时间、温度下进行杂交，分别在不同酶作用时间和显色时间进行分析，比较不同条件芯片检测结果。

Sigma factor is a component of the bacterial RNA polymerase holoenzyme which is composed of RNA core enzyme and Sigma factor.

因子是RNA聚合酶全酶的一个组成成分，RNA聚合酶全酶是由核心酶和σ因子组成。

Consequences of the rotation of DNA are illustrated in Figure 9.17 in the twin domain model for transcription. As RNA polymerase pushes forward along the double helix, it generates positive supercoils ahead and leaves negative supercoils behind. For each

超螺旋与转录还有更多的关系，RNA聚合酶转录DNA时，解链和复链同时也发生，如同Fig113中所示，这需要整个转录复合体绕DNA旋转或者DNA自己绕它的双链轴旋转，DNA旋转的后果如Fig11.19所示，图中是一个转录的双结构域模型，当RNA聚合酶沿DNA前移时，在酶前产生正双螺旋，酶后为负的双螺旋；每一个双螺旋转角被RNA跨越后都会在前面产生+1转角，后面产生-1转角。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。