聚合酶

Methods Semi -quantitative reverse transeriptase - polymerase chain reaction was used to examine the expression of BCRP gene in 62 cases of malignant hematologic diseases, BCRP/β2 - MG ratio was defined as BCRP expressible level.

方法应用半定量逆转录-聚合酶链反应，β2微球蛋白基因作内参照，以BCRP／β2-MG的比值作为BCRP基因的相对表达水平，检测62例恶性血液病BCRP基因的表达。

METHODS: Liver fibrosis models were established in 24 SD rats by intragastric administration (7 g/kg) of alcohol (56°, 560 mL/L) every morning, and 10 normal rats served as controls. The rats in model group were killed by exsanguination from femoral vein at 4, 12 and 24 wk. Pathological changes of liver tissues in rats were identified by Masson staining, and the expression of VEGF mRNA was detected by semiquantitative reverse transcription-polymerase chain reaction.

56度的白酒(560 mL/L)平均以7 g/kg的剂量每日早晨灌胃一次制备肝纤维化模型，灌胃4 wk、12 wk及24 wk采用股静脉放血法分别处死大鼠，观察肝脏病理变化并采用半定量逆转录聚合酶链反应检测VEGF mRNA的表达。

The interaction between DNA polymerase and DNA is important to ensure the high fidelity of DNA replication.

DNA聚合酶与DNA的相互作用对于生物体确保DNA复制的正确性具有重要意义。

The technique is requires a thermal cycling process to amplify target DNA strand in vitro, intercalation of labeling into the amplified DNA strand, and detection of the variation of fluorescence intensity of labeling dye during the amplification of DNA strand a Fluorimeter.

此技术，主要需结合温控机构与萤光分析仪，在温控过程进行DNA体外增生，同时监控增生过程中DNA嵌合萤光萤光强度的变化，进行定量分析，实现此技术之仪器被称为即时侦测同步定量聚合酶连锁反应器(Real-time PCR machine)。

Results The serum antibody level reached to 0.71±0.08-0.77±0.06 after genetic vaccine pCD-HCV1(100 μg/mouse) were inoculated into the mice(n=12) three or four times while blank vector pCD-SRα1 could not induce the mice(n=8) to generate antibody response in same way.After the antibody levels in mice(n=8) immunized by pCD-HCV1 had ascended to peak value(0.71), there was no trend of descending during the following 18 weeks of detection(0.68±0.06-0.75±0.07). Specific fragment of HCV cDNA identified by polymerase chain reaction from DNA extracted from the muscles of the mice after pCD-HCV1 had beed inoculated three months.

结果 基因疫苗pCD-HCV1(100 μg/只)3-4次接种小鼠后(n=12)，血清抗体水平达0.71±0.08-0.77±0.06，空载体pCD-SRα1免疫鼠血清抗体阴性；免疫鼠(n=8)抗体水平达高峰后(0.71)，持续检测18周未见下降趋势(0.68±0.06-0.75±0.07)；pCD-HCV1免疫小鼠3个月后，用聚合酶链反应仍可从肌肉组织中扩增出特异性HCV cDNA片段；免疫鼠PBMC对HCV合成肽CP9、基因重组抗原C、E1刺激后均出现增殖反应，刺激指数分别为4.07±1.58、3.88±0.70和3.69±1.30，与pCD-SRα1免疫鼠相比，差异有显著性(P.001)。

Objective To develop a nested-polymerase chain reaction to identify G and P genotypes of group A rotavirus.

目的用巢式聚合酶链反应对A组轮状病毒常见G、P基因型进行检测。

A general transcriptional factor for RNA polymerase II that binds to multiple promoter elements and specific transcription factors.

一种能与多个启动子元件和特异性转录因子结合的RNA聚合酶II通用转录因子。

Gene frequency/genet ; gene amplification/genet ; polymerase chain reaction/methods

基因频率／遗传学；基因扩增／遗传学；聚合酶链反应／方法学

Methods: Atrial tissue was obtained from the right appendage during open surgery in 25 patients with RHD. The mRNA of ACE and ERK2 and AT1R was semi-qualified by reverse transcription-ploymerase chain reaction and normalized to the glyceraldehyde 3-phosphate dehydrogenase. Western blotting analysis was employed to examine the expression of ACE and ERK and AT1R. Atrial collagen volume fraction was detected by Masson's stain.

25例风心病二尖瓣狭窄接受外科手术者，手术时取右心耳处心房组织500mg，通过逆转录—聚合酶链反应技术，以GAPDH为内参照测量ACE、AT1R、ERK2的 mRNA变化，用Western Blotting了解ACE、AT1R、ERK在蛋白水平表达的差异，经免疫组织化学了解胶原纤维容积分数改变情况。

The authors tested the cervical excretions of 50 women by polymerase chain reaction forgonorrhea and compared the results with those tested by gonococcus cultures.

作者采用聚合酶链式反应诊断女性淋病50例，并将PCR与淋菌分离培养比较，结果50例宫颈分泌物PCR检测获得阳性35例，其中20例与常规分离培养法一致，另外15例PCR阳性而分离培养为阴性，此15例临床诊断为淋病。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐