聚合酶

Methods RPE cells were exposed to shear stress of 2 dyne/cm2 for 0, 0.25, 0.5, 0.75, 1, 1.5, and 3 h. Reverse transcription polymerase chain reaction was used to detect the levels of c-fos and c-myc mRNA in RPE cells. One-way analysis of variance was used to compare the differences.

用2 dyne/cm2的剪切力作用于体外培养的人RPE细胞，作用时间分别为0、0.25 h、0.5 h、0.75 h、1 h、1.5 h和3 h，采用逆转录聚合酶链反应观察c-fos和c-myc mRNA的表达变化，并采用单因素方差分析比较各时间点之间表达的差异。

Objective To investigate a rapid diagnosis of ectogenesis bacterial endophthalmitis using polymerase chain reaction.

目的 探讨聚合酶链反应技术快速诊断外源性细菌性眼内炎的价值。

Polymerase chain reaction is amplified and sequenced to determine the type of emm genes.

聚合酶链反应扩增并进行测序从而对emm基因进行分型确定emm基因的型别。

The results showed that PCR for salmonella is simple,quick and sensitive,which is suitabl to apply in clinic quick diagnosis of salmonella enterocolitis.

结果表明聚合酶链反应用于沙门氏菌肠炎诊断，具有简便、快速、敏感等优点，适于临床快速诊断。

Methods:CBFβ-MYH11 fusion transcripts and inv (16) were analyzed by reverse-transcriptase polymerase chain reaction and fluorescence in situ hybridization,respectively,in acute myelomonocytic leukemia(M4) with or without eosinophilia.

用逆转录-聚合酶链反应和荧光原位杂交技术分别检测急性粒-单核细胞白血病(M4)患者的CBFβ-MYH11融合基因转录本和inv(16)。

Objective To identify gene mutation of a epidermolysis bullosa pruriginosa family. Methods Polymerase chain reaction, DNA squencing, multiplex PCR using allele-specific oligonucleotide primers.

采用聚合酶链反应，DNA直接测序以及等位基因特异引物的多重PCR方法，对一个痒疹样大疱性表皮松解症家系40人（其中患者23例）进行基因突变情况检测。

Methods Expressions of MMP2 and TGF-β1 gene from 43 human suprarenal epithelioma patients' carcinoma and tumor-adjacent tissues were detected by semi-quantitative reverse transcription polymerase chain reaction.

应用半定量逆转录－聚合酶链反应检测43例肾透明细胞癌患者癌与癌旁组织MMP2和TGF-β1的表达水平。

Methods: Three aspects were studied in the epithelization of skin of PAI-3 with the techniques of Immunohistochemistry,Immunocytochemistry,RT-PCR and the analysis of PAI-3 in cornified envelope.

采用免疫组织化学(Immunohistochemistry，IHC)、(Immunocytochemistry，ICC)和逆转录-聚合酶链反应(reverse transcription PCR，RT-PCR )技术及KC角质壳的提取，从三个方面研究了PAI-3 在人皮肤上皮化中的表达及作用。

How do the characteristics of the eukaryotic rna polymerases differ in terms of their cellular location and products?

真核生物的三种rna聚合酶在细胞中的定位、合成的产物各具有怎样的特征？

What mechanisms are used to restrict the transcription of each of the three eukaryotic polymerases to a particular subsets of

有怎样的机制能够限定真核生物的每种RNA聚合酶只能转录特定的基因？

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐