聚合酶

The gonadotropin releasing hormone receptor gene was studied as a candidate gene for the prolificacy of Small Tail Han sheep. Eight pairs of primers were designed to detect single nucleotide polymorphisms of exon 1, exon 2 and exon 3 of GnRHR gene in both high fecundity breeds and low fecundity breeds (South African Mutton Merino, Corriedale and Chinese Merino sheep) by PCR-SSCP. Only the products amplified by primers P4 and P7 displayed polymorphisms.

设计8对引物，应用聚合酶链式反应-单链构象多态性技术检测促性腺激素释放激素受体（gonadotropin releasing hormone receptor， GnRHR）基因外显子1、外显子2和外显子3在高繁殖力品种和低繁殖力品种（南非肉用美利奴、考力代和中国美利奴绵羊）中的单核苷酸多态性，同时研究该基因对小尾寒羊高繁殖力的影响。

PCR method and cryptococcus antigen detection were needed to help diagnosis of cryptococcal meningitis. Combined antifungal treatment improved prognosis.

结论隐球菌抗原及聚合酶链反应检测，有助于提高隐球菌性脑膜炎的诊断水平；抗真菌药物的联合应用，有助于提高隐球菌性脑膜炎的治疗效果。

After 240 weeks, the cumulative probability of HBV polymerase mutations was 29%, but the cumulative probability of mutations with virologic resistance was 20% and of mutations, virologic resistance, and ALT elevations was 11%.

在治疗240周后，HBV聚合酶突变、病毒学抵抗和临床抵抗累积率分别为29%、20%和11%。4例患者有轻度肌酐水平升高。

By using Roche kit as standard, the amplification efficiency of different reagents were compared by Roche Light Cycler instrument for establishing real-time PCR reaction system. The primers were designed based on signature sequence of chromosomal DNA fragment and caf 1 gene in plasmid pMT1 of Y.pestis. And sensitivity and specificity assay were done with serial diluted Y. pestis EV76 strain and other bacterias. Then 275 Y.

采用SYBRGreenI随机掺入法，以Roche试剂盒为标准，比较两公司的DNA聚合酶，用鼠疫菌310 0 4建立荧光PCR反应体系；针对鼠疫耶尔森氏菌特异的染色体标识序列和质粒上F1抗原基因设计引物，检测其灵敏度和特异性，以盲测试验进行验证；在此基础上鉴定2 75株鼠疫耶尔森氏菌，并测试该法检测脏器污染模拟标本的灵敏度。

Objective To establish a method for quantitative detection of cytotoxin associated gene A positive Helicobacter pylori by competitive polymerase chain reaction.

目的 建立竞争性聚合酶链反应定量检测幽门螺杆菌细胞毒素相关蛋白基因A的方法。

RESULTS: The polymerase chain reaction product was in concordance with target fragment. The analysis of the gene sequence proved it is the ALR gene. The overall length of the ALR gene was 378 bp, it was highly conservative in many species, for instance the Homo sapiens, the Mus musculus, the Bos Taurus and the Danio rerio.

结果：聚合酶链反应扩增结果与目的片段位置一致，测序结果表明为肝再生增强因子mRNA，基因全长378 bp，在人、牛、小鼠、斑马鱼等物种中高度保守。

Results (1) The total RNA, reverse transcription product and fluorescence labeled cRNA were all of high quality;(2) There were 420 up-regulated genes and 552 down-regulated genes among 2-fold DEGs, including DKK1 (dickkopf homolog 1) which was 5-fold up-regulated;(3) The results of real time RT-PCR, using β-actin as an internal control, showed that the 2-ΔCt values of DKK1 in HCC, PCT and NLT were 0.089 504, 0.007 65 and 0.000 631 respectively.

结果 （1）配对组织总RNA质量高，反转录cRNA及荧光标记质量好；（2）2倍差异表达基因中，上调基因共420个，下调基因共552个，其中包括5倍上调基因DKK1；（3）以β-肌动蛋白为内对照的实时逆转录聚合酶链反应结果提示，DKK1在癌、癌旁和正常肝组织中的2-ΔCt值分别为0.089504、0.00765和

Objective To explore the relationship of temperatures for denaturalization and annealing to the false negative PCR result.

目的 探讨聚合酶链式反应变性及退火温度与产物假阴性之间的关系。

METHODS: The procedure conditions of PRC: 40 cycles of denaturation temperature from 88 ℃ to 97 ℃ for 5 s and 40 s respectively, and the temperatures of anneal and extension was 60 ℃ for 40 s.

聚合酶链反应运行程序条件：变性温度分别为88~97 ℃，变性时间分别为5 s和40 s，退火和延伸60 ℃，40 s，循环数40。

Detection of 98% of DMD/BMD gene deletions by polymerase chain reaction.

应用14对引物多重聚合酶链反应作假肥大型肌营养不良症的产前基因诊断。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐