聚合酶

In later stages of the disease, a process called polymerase chain reaction can be used to confirm diagnosis.

在稍后阶段的疾病，这个过程称为聚合酶链式反应可以用来确定诊断。

Objective To study the value of polymerase chain reaction in prenatal diagnosis of alpha thalassemias.

目的 研究聚合酶链反应技术在α地中海贫血产前诊断中的价值。

Methods The expression of mdr1 and MRP were measured in 55 patients with AL by reverse transcription polymerase chain reaction.

采用逆转录-聚合酶链反应的方法检测55例AL患者的mdr1及MRP的表达。

Magna populations in edge and central region was studied. In 39 A. magna samples and five A. chukar outgroups, the entire mtDNA D loop was PCR amplified. The 456-457 nucleotides of the DNA D loop domain Ⅰ have been sequenced by dideoxy chain termination method. The 16 variable sites(3 5% of the entire sequence) defined 15 haplotypes.

本研究采用聚合酶链式反应和直接测序的方法获得了采自甘肃的大石鸡一个边缘种群和两个中心地理种群共39个个体的线粒体DNA控制区基因456～457个核苷酸的基因序列，16个变异位点(占整个序列的3.5%)有15个单倍型。

The level of serum amylase, the pulmonary dry/wet weight ratio and histological changes were measured at different time points in each group. The expression of pulmonary CINC protein and mRNA were detected by immunohistochemistry and semi-quantitative reverse transcriptase polymerase chain reaction, respectively.

分别检测各组不同时间点血清淀粉酶、肺干湿重比和肺组织病理学改变，用免疫组织化学法和半定量逆转录聚合酶链反应法检测肺组织中CINC蛋白和CINC mRNA表达的变化情况。

METHODS: The acute pancreatitis model was induced by retrograde injection of 50 g/L sodium taurocholate into the bili-pancreatic duct in SD rats. Forty-eight mas-culinity SD rats were randomly divided into 2 groups: the sodium taurocholate group and the operational control group. The serum amylase level and the histol-ogical pathologic changes of pancreas were measured at different time point in each group. The expression of pancreatic CINC protein and mRNA were determined by immunohistochemistry and semi-quantitative reve-rse transcriptase polymerase chain reaction, respectively.

采用向胆胰管逆行注射50 g/L牛磺胆酸钠建立大鼠SAP模型， 48只♂SD大鼠随机分为手术对照组和SAP组，分别检测各组不同时间点血清淀粉酶和胰腺组织病理学改变，用免疫组织化学法和半定量逆转录聚合酶链反应法检测胰腺组织中CINC蛋白和CINC mRNA表达的变化情况。

The method of detecting coliform using Polymerase Chain Reaction is established.

建立了饮用水大肠杆菌的聚合酶链式反应检测方法。

Objective To establish a method for rapid detection of clinical suspect fungal corneal ulcer by polymerase chain reaction.

目的 探讨聚合酶链反应快速检测在真菌性角膜溃疡诊断中的价值。

Methods The mutations in blood corpuscle mitochondrial DNA T2DM without consanguinity and controls were determined by polymerase chain reaction-restriction fragment length polymorphism.

采用聚合酶链反应-限制性片段长度多态性法对无血缘关系的糖尿病患者及正常对照个体的血细胞线粒体DNA进行突变分析。

MAIN OUTCOME MEASURES: The results of recombinant plasmid PCR and gene sequencing, the correlation coefficient of the standard curve and the repeatability of the plasmid standard.

主要观察指标：重组质粒聚合酶链反应和基因测序结果，标准品标准曲线的相关系数及标准品的重复性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐