聚合酶

Methods Multiplex nested PCR was used to amplify the sex linked amelogenin and IL-3 genes from human single lymphocytes and single blastomere.

方法取成人外周血单个淋巴细胞和单卵裂球，采用多重巢式多聚合酶链技术同时扩增性别相关基因Amelogenin基因和IL-3基因。

Characterization of complementary DNAs derived from braconid wasp ovaries identified genes encoding subunits of a viral RNA polymerase and structural components of polydnavirus particles related most closely to those of nudiviruses—a sister group of baculoviruses.

基因编码的RNA聚合酶亚组和多去氧核糖核酸病毒粒子的结构组件，与nudiviruses有着密切的联系。而通过来自小茧蜂卵巢互补DNAs的表征可以将两者识别出来。

To measure the area of VEGF expression in the bronchiole and pulmonary artery. To detect the expression of VEGF mRNA With RT-PCR.

采用免疫组化和逆转录－聚合酶链反应检测VEGF蛋白质和mRNA的表达。

Methods The experimental TBI model was established by bumpiness of free falling body according to Feeney′s.The rats′cerebral edema were imaged with magnetic resonance image,The changes of brain water content and permeability of blood-brain barrier were measured by the methods of wet and dry weight and Evans blue fluorometry.The expression of AQP-4 mRNA was examined by reverse transcriptase polymerase chain reaction.

按照改进的Feeney自由落体撞击法建立大鼠创伤性脑损伤模型，用磁共振成像对大鼠脑水肿进行检测，干/湿比重法和伊文思蓝测定法观察大鼠TBI后不同时相脑组织含水量和BBB通透性的变化，并采用逆转录聚合酶链反应法检测AQP-4 mRNA的表达，蛋白质免疫印迹法检测AQP-4蛋白的表达。

Based on the conservation of miRNAs sequence, we compared the known miRNAs among five mam-mals, i.e., human, mouse, cattle, pig and dog with the sequence of sheep genome that is highly homologous to goat genome, published on the NCBI, and 11 candidate miRNAs were eventually obtained. RT-PCR analysis showed the expression of the 11 miRNAs in brain and 5 in liver, indicating that they might be novel miRNAs. The methodology provides an alternative approach to the exploration of new miRNAs in goat.

根据miRNA分子具有一定的保守性，文章将人、小鼠、牛、猪和狗5种哺乳动物已知的miRNA分子与NCBI公布的与山羊具有极高同源性的绵羊基因组序列对比，获得11条miRNA候选分子，然后通过逆转录聚合酶链反应验证，发现在山羊脑组织中这11条分子均有表达，肝脏组织中有5条分子表达，初步确定为山羊新的miRNA分子，为寻找山羊miRNA提供了新的思路。

The morphology of the caudal side were investigated by HE staining.

采用反转录-聚合酶链反应检测脑损伤区AQP4的表达。

Histone deacetylase 6(HDAC6) is microtubule and HSP90 associated protein, which control genetic transcription and change cellule motor ability by deacetylased microtubule and HSP90,and then participate tumorigenesis, growth, metabasis, and the production of tumor vessel.

研究方法： 1。应用激光捕获显微切割术联合逆转录-聚合酶链反应技术，检测36例行根治性切除的原发性肝细胞癌中心处非坏死癌组织及24例正常肝组织的HDAC6 mRNA在组织中的表达，并对HDAC6与肝癌发生发展过程中相关的临床病理因素间相关性进行研究。

Polymerase chain reaction analysis of cerebrospinal fluid is the gold standard for the diagnosis of herpes simplex encephalitis.

对脑脊液的聚合酶链反应是诊断单纯疱疹性脑炎的金标准。

Objective: To investigate the clinical value of Polymerase Chain Reaction in the diagnosis of trachoma.

目的：探讨聚合酶链反应在沙眼诊断中的临床应用价值。

Methods The genotypes of 152 patients with CI were detected by polymerase chain reaction and compared with control group.

应用聚合酶链反应技术，检测 15 2例CI患者的基因型，并与对照组比较。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。