聚合酶

And then HepG2.2.15 cellswere cultured with different non-cellulotoxic concentration ofα-amanitin or 3TC for six days.

采用MTT法检测不同浓度α-鹅膏毒肽对体外培养的HepG2 2.2.15细胞株的毒性，然后在无毒浓度下用不同浓度α-鹅膏毒肽或拉米夫定与HepG2 2.2.15细胞共培养6天，分别在第3天和第6天收集细胞培养上清液，采用时间分辨免疫荧光法测定上清HBsAg、HBeAg浓度；收获细胞提取总RNA，行逆转录聚合酶链反应检测细胞内HBsAg和HBcAg mRNA的表达。

The Culture of Green beaus in extract solution of seven poisonous mushrooms resulted in different effects. There are obvious difference among the content of the proteins in Green beaus which were cultured in extract solution of seven poisonous mushrooms, and it is thought that α-amanitin in extract solution of these mushrooms can inhibit the function of RNA polymerase Ⅱ in eukaryoctic cells and further inhibit the synthsis of protein.

用鹅膏菌属含α-毒伞肽的毒素粗提液培养新鲜绿豆，结果在36小时后，各种不同毒苗的毒素粗提液培养的绿豆生长情况有明显不同，用紫外吸收法测定蛋白质含量，发现毒素粗提液培养的绿豆细胞中蛋白质含量比蒸馏水培养的有明显下降，这表明α-毒伞肽的作用机理的确是通过抑制RNA聚合酶Ⅱ而寻致蛋白质合成减少。

Amato and Russello applied a straighiforward polymerase chain reaction test to determine the sex of all the captive St.

阿马托和罗素采用一种直接的聚合酶链锁反应试验来确定所有从圣文森特小岛和巴巴多斯捕获的79只圣文森特亚马逊鹦鹉的雌雄。

Methods Turning tests induced by apomorphine and forelimb functional tests were performed. Also, the expression of TH mRNA in mesencephalon of the rats was detected by semi-quantitative analysis of one step RT-PCR.

利用阿朴吗啡诱发旋转试验和前肢功能试验，检测PD大鼠的神经行为学特点，一管法逆转录聚合酶链反应半定量检测其中脑TH mRNA的表达。

Endometrial carcinoma ; human expression array ; gene expression ; polymerase chain reaction

子宫内膜肿瘤；人表达阵列；基因表达；聚合酶链反应

Methods Sequence specific primers-polymerase chain reaction was used for the detection of genotypes and alleles of rs3850641 A/G loci in OX40L gene in 187 PACS cases (≤55 years) and 138 controls. Diseased vessel numbers and stenostic severity integral by coronary arteriogram and serum levels of sOX40L, C-reactive protein and vascular cell adhesion molecule-1 (VCAM-1) by enzyme-linked immunosorbent assay and nephelometry were measured. The relationship between OX4OL gene variants and PACS risk, SSI as well as plasma levels of three cytokines were analysed.

采用聚合酶链反应－序列特异性扩增技术检测187例年龄≤55岁、早发急性冠状动脉综合征患者和138例冠状动脉造影正常者rs3850641位点A/G变异的基因型、等位基因及其分布频率；采用冠状动脉造影测量病变血管支数和狭窄程度积分；应用ELISA法测试血浆sOX40L、血管细胞黏附分子(VCAM-1)；应用散射比浊法测试血浆C反应蛋白水平；分析OX40L基因变异与ACS风险、冠状动脉病变程度和血浆3个细胞因子之间的关系。

A rapid and sensitive method used for the detection of staphylococcus aureus in Jinhua ham was established.

建立了一种用于金华火腿中金黄色葡萄球菌的聚合酶链式反应检测方法。

The relationship between this system and serum autoantibody levels was also studied.

采用逆转录聚合酶链反应方法检测18例活动期LN患者、16例非活动期LN患者和10名正常人PBMCs内IL-6、gp80与gp130的mRNA表达水平。

Methods Cadmium-specific promoter PcadA was obtained by PCR from pPcadA and was ligated to E.coli-B.subtilis shuttle vector pNGFP with gfp being reporter gene ,after which the recombinant plasmid was transformed into 1A751 strain to form the bio-engineered bacteria.

采用聚合酶链式反应从质粒pPcadA上扩增得到重金属镉离子特异性启动子PcadA，以绿色荧光蛋白基因为报告基因，以大肠杆菌-枯草杆菌穿梭载体pNGFP构建了绿色荧光蛋白镉离子诱导表达载体。

F508 mutation detection on the single blastomere was performed with nested polymerase chain reaction.

同时利用套式聚合酶链反应技术，对活检所得的单个卵裂球分别进行ΔF508 突变基因检测。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。