聚合酶

Banana bunchy top virus; component Ⅰ; replicase gene

香蕉束顶病毒；聚合酶链反应；复制酶基因

The expression of cytochrome P450 3A (CYP3A) subfamily implicated in drug metabolism in human fetal adrenals and livers was detected by using enzyme assays and the reverse transcription polymerase chain reaction.

采用酶学和逆转录聚合酶链式反应技术，研究与药物代谢密切相关的细胞色素P450 3A( CYP3A)在胎肾上腺和胎肝中的表达。

Component of prokaryotic RNA polymerase holoenzyme.

原核生物RNA聚合酶全酶的组成成分。

Methods The method of PCR-based ligase detection reaction was applied to genotype rs1549637 of PLA2G4C gene among 403 schizophrenic and 400 healthy people.

采用聚合酶链式反应和连接酶检测的方法，在中国北方汉族403例精神分裂症患者和400例健康对照者的血液样本中检测PLA2G4C基因上的 rs1549637位点的基因型。

Using the total RNA of merozoites of Eimeria acervulina isolated from Guangdong Pro- vince as template,a partial segment of 3-1E gene was amplified by RT-PCR. The gene fragment 682 bp inlength was cloned into pGEM-T Easy vector,and the recombinant plasmid was identified by PCR,restric-tion enzyme analysis and sequencing. The homology analysis revealed that the nucleotide homology be-tween the 3-1E gene of the E.

根据GenBank中登录的堆型艾美球虫3-1E基因序列，设计了3条引物，以广东株堆型艾美球虫裂殖子总RNA为模板，利用反转录-聚合酶链反应扩增获得了3-1E基因部分片段，将这一片段克隆至pGEM-T Easy载体中，经PCR、限制性内切酶分析和克隆片段的序列测定、比较，证实了克隆片段的可靠性。

The uptake of 〓I-OxLDL in adipocytes was determined by gamma ray counter and normalized to protein concentration of each sample, was expressed as nanograms of 〓I-OxLDL uptake per milligram of cell protein. Reverse transcription polymerase chain reaction was used to evaluate PPARγ and CD36 mRNA expressions.

采用酶联免疫吸附法检测血浆IL-6以及脂肪细胞分泌IL-6的水平；采用gamma射线计数仪来检测脂肪细胞对〓I-OxLDL的特异性摄取情况，采用改良Lowry法进行细胞蛋白浓度测定，细胞对〓I-OxLDL的特异性摄取量以ng／mg细胞蛋白表示；采用逆转录聚合酶链式反应来检测PPAR γ和CD36 mRNA在脂肪细胞的表达情况。

Quantitative RT-PCR and hybridization methods were used to deter mine the effect of inhibition of HCV by ribozymes.

CD-SRα中，转染HCV感染的MT-2细胞，用定量逆转录-聚合酶链反应、核酸杂交等方法测定核酶对HCV的抑制作用。

Quantitative RT-PCR and hybridization methods were used to deter mine the effect of inhibition of HCV by ribozymes.

CD-SRα中，转染HCV 检查感染的MT-2细胞营养，用定量逆转录-聚合酶链反应、核酸杂交等方法测定核酶对HCV的抑制作用。

Crohn's disease was induced by trinitrobenzene sulfonic acid in the model group. The mice were sacrificed 5 days afterwards and LPMC were isolated and cultured. The expression of CCR2 and CCR5 on LPMC were determined by flow cytometry. The secretion of MIP-1α in the supernatant was determined by enzyme-linked immunosorbent assay, and the expression of MIP-1α mRNA was determined by reverse transcriptase polymerase chain reaction.

模型组小鼠以三硝基苯磺酸灌肠造模。5d后处死，提取和培养LPMC，采用流式细胞仪测定小鼠LPMC中趋化因子受体CCR2、CCR5的表达；采用酶联免疫吸附测定检测培养上清液中MIP-1α的分泌；采用逆转录聚合酶链反应检测MIP-1α mRNA的表达。

Hepatitis B virus ; Covalently closed circular DNA ; Polymerase chain reaction ; Mung bean nuclease

肝炎病毒；乙型；共价闭合环状DNA ；聚合酶链反应；绿豆芽核酸酶

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。