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Methods After L-02 cells were incubated with 40, 80, and 160μg/ml sodium fluoride for 24 hours, the cell survival rate, the level of P53 protein and mRNA expression was assessed by MTT, Western blot and RT-PCR analysis respectively. The cell survival rate in L-02 cells treated by high concentration of fluoride was significantly lower than that in the control group.

方法]对体外培养的人胚肝细胞染毒氟化钠40、80、160μg/ml 24h后,分别采用MTT试验、蛋白印迹技术和逆转录-聚合酶链式反应方法检测人胚肝细胞存活率、P53蛋白和mRNA水平。

Objective To evaluate specimen processing with sodium dodecyl sulfate on the detection of Mycobacterium tuberculosis by PCR-reversed dot hybridization.

目的探讨应用十二烷基硫酸钠处理标本在结核分支杆菌聚合酶链反应中的价值。

Methods: Real time FQ - PCR testing and germiculture methods adopted for NG,CT,UU testing on spermatic fluids of 105 cases of male infertility and regular spermatic fluid testing.

采用实时荧光定量聚合酶链反应技术对105例男性不育者的精液进行NG、CT、UU检测并做精液常规检查。

The results showed that the SiO2-g-PS nano-microsphere had a structure of sphericity composed of PS and SiO2 as core and shell respectively.

结果表明,乳液聚合产物基本呈球形、SiO2为核、PS为壳的核壳结构。

Methods The antibody of Borrelia burgdoferi in the crowd serum was detected by immunofluorescence, and the spirochete DNA was tested with PCR.

情况。方法人群血清伯氏疏螺旋体抗体的检测采用免疫荧光法,以聚合酶链反应检测伯氏疏螺旋体DNA。

The number and position of substituent on ligand of metallocene has strong effect on copolymerization ability.

发现非桥联茂金属配体上取代基的位置和数量对共聚合能力影响很大。

Three methods are used to prepare molecular imprinted polymers,which include conventional bulk technique or polymerization with ultraviolet initiated in low temperature and polymerization with silica-gel as sacrificial materials. In the polymerization procedure , Sulfamethazine(SM2)and Sulfisoxazole were used as target template molecules, methacrylic acid and 4-vinylpyridine(4-VP) and acrylamide were selected as functional monomers, ethylene glycol dimethacrylate as crosslinker, azobisisobutyronitrile was used as initator.

主要工作如下:首先,以磺胺异噁唑和磺胺二甲嘧啶为模板,分别以4-乙烯基吡啶,甲基丙烯酸,丙烯酰胺为功能单体,以二甲基丙烯酸乙二醇酯为交联剂,偶氮二异丁腈为引发剂,采用了传统方法(60℃恒温水浴)、牺牲硅胶法及低温紫外光聚合法等三种方法合成了一系列的磺胺类药物印迹聚合物。

The bulk and solution photopolymerization of vinyl butyl ether were studied using diph-enyl-4-thiophenoxyphenyl sulfonium salt as photoinitiator.

以二苯基-4-苯硫基代苯基硫鎓盐为光引发剂,研究了乙烯基正丁醚的本体和溶液光聚合。

Therefore : Offer web services interfaces and content syndication, and re-use the data services of others.

因此:提供网络服务界面和内容聚合,并重用其他人的数据服务。

The use of syndication technologies, however, creates many more alternatives.

然而,聚合技术的使用,创造了更多选择。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。