聚合的

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

In terms of grammar , oppositeness is one of the most complicated paradigmatic relationships , which has been much elaborated and explored by many linguists .

在语法上，反义关系作为纵聚合关系中最复杂的一种关系而被许多语言学家所研究。

The result of orthogonal experiment showed that the sequence of the factors affecting extraction ratio of the LPC was flocculation method, solid-liquid ratio and the amount of adding salt. The optimal combination condition was solid-liquid ratio 1:7, salt concentration 0.3% and ethanol precipitation method.

对固液比、盐浓度、凝絮方法3因素3水平正交试验结果表明，影响聚合草叶蛋白提取率的因素依次为凝絮方法＞固液比＞加盐量，最佳条件组合为固液比1：7、盐浓度0.3%、乙醇法沉淀。

The parts that are not overlapped means different division of labor and contradictions between the two.

这 两个职责不同的安全核心，其内部关系高度制度化和组织化，聚合程度高，稳定性强。

An agglomeration multigrid method was applied to numerical calculation of rotor forward flight flow based upon the unstructured dynamic overset grids.

基于非结构动态嵌套网格，将聚合多重网格方法引入到旋翼的前飞流场计算中。

Objective To evaluate the value of pancreatic juice in cytologic examination and PCR-SSP in detecting K-ras gene mutation for diagnosis of pancreatic carcinoma.

目的 评价胰液细胞学检测与聚合酶链反应―顺序特异性引物检测K-ras基因点突变诊断胰腺癌的价值。

The number-average molecular weight and molecular weight distribution of PCL was measured by GPC.

通过凝胶渗透色谱法测定了聚合产物的数均分子量及其分布。

The molecular weights of obtained PDTC and PCL were 25.4×10〓 and 21.5×10〓, respectively.

聚合产物分子量高，PDTC和PCL均聚物的分子量分别可达25.4×10〓和21.5×10〓。

Phylogenetie analysis shows that the two pearl oyster species are most closely related, supporting the traditional classification.

系统发育分析表明两者的亲缘关系最近，与传统分类相符，然后与太平洋牡蛎等聚合在一起。

The aggregate data from all the personal schedules also helps the conference organizers.

将所有人的个人日程安排聚合到一起同样会对会议组织者有帮助。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐