聚合的

Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200232,ChinaAbstract: Nine samples from swine abortus were detected by RT-PCR with a pair of primers specific to NS1 gene of Japanese encephalitis virus.

采用反转录聚合酶链式反应方法检测猪流产胎儿病料中的乙型脑炎病毒，并对阳性病料做病毒分离与纯化。

Effects of three feeding methods on siloxane modified acrylate emulsion polymerization stability and properties of emulsion were studied.

研究了3种单体投入方式对有机硅改性丙烯酸酯乳液聚合稳定性及性能的影响。

For this type of new model,we study it mainly from these ways:First,we make further research on the nature features of their distributions;give out the probability distribution of the mixed stochastic variables,and the statistical features and the approximate distributions of the short-term individual risk models. Also the relationships between the total amount of compound compensation and individual one were investigated.At last we give out a kind of practical calculation method for the individual risk model.That is the calculation of the number of claims translating into the calculation of Vandermonde determinant.Second,a kind of risk model with main claims and by-claims is built under specific conditions.

对于这类有主副理赔的风险模型，我们主要从以下几个方面进行研究：首先，对短期个别风险模型和长期聚合风险模型的理赔总额的分布性质及特征做了较深入的研究，给出了混合型随机变量和的概率分布和短期个别风险模型理赔总额的统计特征与近似分布；在复合分布的框架下，研究了聚合风险模型理赔总额和个别理赔额之间的分布关系，给出了和个别理赔额对应的理赔次数的实用计算方法，即在总索赔额服从复合泊松分布时，把不同理赔额对应的理赔次数的计算转化为线性方程组的计算。

Nanoparticle of an appropriate proportion were homogeneously dispersed in the PI matrix, and the interfacial adhesion between the matrix and the nanoparticle was hence strong owing to the high specific surface area and reactivity of the nanoparticle. Subsequently, the molecular chains of PI attained unchanged or even increased interaction at a proper mass fraction of the nanometer particle, which contributed to improve the wear-resistance of the nanocomposites.

采用PI的原位聚合填充方法，利用聚酰亚胺与无机纳米表面较强的相互作用（如静电作用、较强的范德华力、氢键甚至化学键的形成），用非水悬浮工艺制备了无机纳米/聚酰亚胺纳米复合材料，率先用大型分析仪器全面地表征了复合材料的结构，采用原位聚合填充法制备的聚酰亚胺复合材料中，无机纳米分散状态良好；无机纳米氧化物/聚酰亚胺复合材料的结构表征显示出PI有机相和SiO〓无机相之间形成了键联型复合体系，两相相互影响；在复合体系中，单相本体的结晶结构并未发生改变；在摩擦过程中纳米氧化物没有发生价态的转变；无机纳米氧化物的加入，提高了聚酰亚胺的耐热等级；适量纳米氧化物并不影响复合材料的机械性能， 2。

Emulsification is the mainly reason to influence on the reaction, the influences of emulsifiers such as the concentration of emulsifies, the ratio of anionic to nonionic emulsifier on the polymerization stability were researched;The influences of concentration and feeding method of initiators on the reaction stability and the mechanical properties of coating film of acrylate polymerization stability were researched;The influences of reaction temperature, stirring speed and pH buffer agent on the polymerization stability and pH regulator on the storage stability of Acrylate emulsions were also researched.

乳化剂是影响乳液稳定性的主要因素，因此对乳化剂进行了全面的探讨，包括乳化剂的种类和用量、阴离子型与非离子型乳化剂的比例对乳液稳定性的影响；文中还研究了引发剂的用量、加入方式对聚合稳定性的影响，以及引发剂用量对聚丙烯酸酯涂膜力学性能的影响；此外，讨论了反应温度、搅拌速率、pH值缓冲剂对乳液聚合过程稳定性的影响及pH调节剂对乳液贮存稳定性的影响。

The hydrolysates from oat xylan using Xyn Ⅰ Xyn Ⅱ and their mixture for hydrolysis were analysed by HPLC. It was showed that Xyn Ⅰ hydrolyzed mainly the unsubstituted regions of oat xylan, and hydrolytes were high xylooligosaccharides; whereas Xyn Ⅱ exhibited greater catalytic versatility than Xyn Ⅰ and were able to attack substituted regions of the polysaccharide and showed greater activity to low xylooligosaccharides than Xyn Ⅰ to give xylobiose as the main hydrolysate. The purified xylanases were acidic enzymes.

以纯化酶组分及其混合物水解燕麦木聚糖，采用高效液相色谱分析相应的水解产物，结果表明，Xyn Ⅰ降解燕麦木聚糖时，主要降解作用发生在底物中没有取代基的区域，水解产物的聚合度较高；Xyn Ⅱ对底物具有更强的适应性，能降解底物中有取代基的区域，相对Xyn Ⅰ酶组分，Xyn Ⅱ酶组分对低聚合度的木聚糖的活性更高，木二糖为主要降解产物。

The morphology of virions seems to not be change after incubated with the paeonol by electron microscope. The regained infectivity of TMV recovered by dialysis from incubation with paeonol in vitro showed that the paeonol have not direct viricidal effect. The paeonol did not reduce infectivity when applied before TMV inoculation. The paeonol in low concentration can restrain the polymerization of TMV coat protein in vitro. The paeonol did not reduced infectivity of TMV-RNA in vitro. It is proposed that the paeonol inhibited TMV by binding with TMV coat protein to interfere with recognition of a host receptor essential for infection or by interfering TMV-CP polymerization in plants. The anti-TMV activity of low molecular weight chemicals extracted from Polygonum cuspidatum were determined.

丹皮酚与TMV混合，电镜下观察对粒体形态没有影响；TMV与丹皮酚混和后透析可恢复侵染力，表明丹皮酚对病毒粒体并没有直接毒害作用；预先用丹皮酚喷施叶面不影响病毒的侵染，表明丹皮酚未干扰TMV对叶片侵染位点的识别；丹皮酚在低浓度时可阻碍TMV-CP的体外聚合，对TMV-RNA并无体外抑制侵染作用，据此推测丹皮酚对TMV的抑制作用可能主要通过其与TMV-CP结合影响病毒粒体对叶片表面侵染位点的识别，或影响体内TMV粒体的脱衣壳或TMV-CP亚基的体内聚合。

It was demonstrated that the actin could be polymerized into discrete filaments of tree-like branch structure, random coil filaments cluster and long filaments with different diameters in F-buffer besides random aggregates. These polymerized filaments clearly exhibited the structural polymorphism and showed obvious difference from those assembled under the regulation by actin binding proteins or phalloidin.

研究发现，actin在体外通过自装配过程除了形成无序的蛋白堆积物之外，还能够聚合形成复杂的离散结构，包括树状分支的纤维丛、无规卷曲的纤维簇以及具有不同直径的长纤维等；这些大尺度纤维复合物明显不同于在ABPs或过量F-actin稳定剂参与下形成的由单根微丝和微丝束构成的聚合结构。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Sediments of this system were sequentially extracted in natural granularity. The result showed that there was a good linear relationship between organic matter and org / bact P contents in sediments. This organic matter in sediments was the source of org / bact P. The total phosphorus in sediments dominated the content of total dissolved phosphorus and phosphate in interstitial water. In addition, there were good relatiohships between total phosphorus in sediment and the total dissolved phosphorus in interstitial water, and between total phosphorus in sediment and phosphate in interstitial water. FeP and AlP in sediments were correlative with Eh and phosphate in interstitial water, respectively. This showed that the FeP and AlP contents were affected by Eh in interstitial water and the total content of FeP and AlP dominated the content of phosphate in interstitial water.

对沉积物—间隙水中磷形态的连续提取分析发现，自然粒度下，沉积物中有机质含量与沉积物中有机/细菌聚合磷存在很好的线性相关性，说明沉积物中有机质是有机/细菌聚合磷的源；沉积物中总磷决定间隙水中总溶解性磷和溶解性磷酸盐的含量，并且总磷与间隙水中总溶解性磷和溶解性磷酸盐存在很好的相关性；沉积物中FeP和AlP分别与间隙水中的Eh和溶解性磷酸盐存在相关性，说明Eh的大小严重影响FeP和AlP的含量，但是FeP和AlP含量之和又主导着间隙水中溶解性磷酸盐的含量。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。