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objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr,用双向凝胶电泳技术分离总蛋白,银染及胶体考马斯亮蓝染色,image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱,两种染色凝胶相比,硝酸银染色在样品少时显示更佳,过量则影响图像质量,而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加,并无明显拖尾。结论两种显色方法受样品量影响较大,恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

Main products are plastic-blown film series, including the two-story, three-tier co-extruded-blown film, blown film for agricultural plastic film, polypropylene blown film Machine, PVC shrink-blown film and other plastic film bag-volume series, including even the point of breaking bag, vest-pocket bag, garment bag and the bag of large garbage bags and other products registered trademark "of the licence -."

主要产品有塑料吹膜机系列,包括二层、三层共挤吹膜机,农地膜吹膜机,聚丙烯吹膜机,PVC收缩膜吹膜机等;塑料薄膜制袋机系列包括连卷点断制袋机,背心袋制袋机,服装袋以及大型垃圾袋制袋机等,产品注册商标&联宇牌&。

It was hard to treat and make it reach the national standard for reuse water or discharge water with conventional treatment processes and equipments. Waste water containing hydrolyzed polyacrylamide was treated with gas flotation and the biological touch oxidized method.

用气浮+生物接触氧化法对孤岛油田含聚污水进行处理,首先利用气浮去除悬浮油和大部分聚合物,然后利用生物接触氧化膜上的烃类降解菌和聚丙烯酰胺降解菌组成的高效降解菌群的生物降解作用去除乳化油和残余的聚合物。

They are hydrogen peroxide, ammonium persulfate, sodium persulfate, chlorinated polypropylene, chloridized EVA, garox mek, carbon dioxide, hydrogen, sodium carboxymethyl cellulose, carboxymethyl amylum sodium, feed additive, auric salt and silver salt series, tin salt series, tin dichloride, silver nitrate, fireworks raw materials, spheroid form nickel hydroxide, refined sulfuric acid, battery sulfuric acid, ultramicro fine powder, compound fertilizer, 40% multiple sulfur suspending agent, 40% sulfur-tricyclazole suspending agent, 40% sulfur-multiple-trideflocculant, 20% pemiro-dichlor suspending agent, high panniculus adiposus and so on.

主要产品有:过氧化氢、过硫酸铵、过硫酸钠、氯化聚丙烯、氯化EVA 、过氧化甲乙酮、二氧化碳、氢气、羧甲基纤维素钠、羧甲基淀粉钠、饲料添加剂、金、银盐系列、锡盐系列、氯化亚锡、硝酸银、烟花原料、球形氢氧化镍、精制硫酸、电池硫酸、超微细粉体、复合肥、40%多硫悬浮剂、40%硫·三环唑悬浮剂、40%硫·多·三悬浮剂、20%吡嘧·二氯悬浮剂、高脂膜等10个系列百多个品种、规格产品。

Made of polypropylene, Soul jars are currently available in 5-, 15-, and 50-ml thick-walled versions, as well as a 100-ml size.

聚丙烯,魂瓶,目前可在5 -,15 -,50毫升厚壁的版本,以及100毫升。

Companies operating various types of professional domestic plastic raw materials imports, mainly polypropylene, high-low pressure polyethylene, linear polyethylene, polystyrene, polyvinyl chloride, sulphur and some engineering plastics, turnover amounted to three billion Yuan.

公司专业经营各类进口国产塑料原料,主要有聚丙烯、高低压聚乙烯、线性聚乙烯、聚苯乙烯、聚氯乙烯、部分工程塑料及硫磺,年营业额达到叁亿多元人民币。

The company mainly produces PAM which plays roles in oil producing, waste water cleaning, drinking water cleaning, mining, sugar making, paper manufacturing, and so on.

公司主要生产经营用于油田、污水处理、饮用水、采矿、制糖和造纸等领域的国内外聚丙烯酰胺系列产品。

No weld plastic storage tanks, motor transport tank, tank transport ship products using the international advanced one-time model of rotating plastic molding process, the product has a total of polyethylene forming a seamless, impact resistance, anti-aging, weight Light, non-leakage, acid, long life, consistent with the health standards, etc., the use of the performance is much better than the traditional glass containers steel, PVC welding containers, polypropylene containers welded, steel-lined rubber, steel-lined steel containers such as glass, ordinary life is a traditional more than twice the FRP, GRP impact is more than three times, to a large extent can be replaced by stainless steel, titanium and other m e t al containers, is the best of today's new generation of high-performance corrosion-resistant chemical liquid storage transportation equipment, and cheap.

无焊缝塑料储罐、汽车运输贮罐、船舶运输贮槽系列产品采用国际先进的旋塑工艺一次性模式成型,产品具有整体聚乙烯一次成型无接缝、抗冲击、抗老化、重量轻、不渗漏、耐酸碱、寿命长、符合卫生标准等优点,使用性能大大优于传统的普通玻璃钢容器、聚氯乙烯焊接容器、聚丙烯焊接容器、钢衬橡胶、钢衬玻璃钢等容器,使用寿命是传统普通玻璃钢的二倍以上,抗冲击是玻璃钢的三倍以上,在相当大的范围内可取代不锈钢、钛等金属制品容器,是当今最理想的新一代高效的化工液体耐腐蚀贮运设备,且价廉物美。

This series of products adopt international advanced one-time model of rotating plastic molding process, the product has a total polyethylene forming a seamless, impact resistance, anti-aging, light weight, non-leakage, acid, long life, consistent with the health standards advantages, the use of the performance is much better than the traditional glass containers steel, PVC welding containers, polypropylene containers welded, steel-lined rubber, steel-lined steel containers such as glass, ordinary life is a traditional glass fiber reinforced plastic for more than twice, impact glass steel more than tripled, to a large extent can be replaced by stainless steel, titanium and other m e t al containers, is the best of today's new generation of high-performance liquid chemical storage and transportation equipment, corrosion resistance, and good value for money.

本系列产品采用国际先进的旋塑工艺一次性模式成型,产品具有整体聚乙烯一次成型无接缝、抗冲击、抗老化、重量轻、不渗漏、耐酸碱、寿命长、符合卫生标准等优点,使用性能大大优于传统的普通玻璃钢容器、聚氯乙烯焊接容器、聚丙烯焊接容器、钢衬橡胶、钢衬玻璃钢等容器,使用寿命是传统普通玻璃钢的二倍以上,抗冲击是玻璃钢的三倍以上,在相当大的范围内可取代不锈钢、钛等金属制品容器,是当今最理想的新一代高效的化工液体耐腐蚀贮运设备,且价廉物美。

The influences of steel fiber and polypropylene fiber on the mechanical properties of pavement concrete were investigated in this paper.

研究了钢纤维和聚丙烯纤维对道路混凝土力学性能的影响。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。