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According to the result, we believed that the reason resulted in the low sensitivity was that the marker residue compound MQCA structure has changed after conjugated with protein and the antibody induced by the conjugation could not recognize the free MQCA in the ci-ELISA. Therefore we designed some hapten which could reserve the marker residue structural speciality mostly. After synthesis route selection and optimum, the hapten 7-Hydroxymethyl-3-methyl-quinoxaline-2-carboxylic acid was synthesized, used 4-methyl-2-nitroaniline as the start material via six steps, such as oxidation cyclization, bromize reaction, hydrolysis, Beirut reaction, reduction, hydrolysis. This work established basis for producing the high affinity antibody and developing the sensitive ELISA residue detection method.

本论文认为导致ELISA检测灵敏度较低的原因,是残留标示物和蛋白偶联后的特征结构发生改变,诱导产生的抗体不能够对游离MQCA很好的识别,因此,本论文设计了能够最大化保留MQCA结构特征的系列半抗原结构,并对其合成路线进行了筛选和优化,最终以对甲基邻硝基苯胺为起始原料,经氧化环化、溴代、水解、Beirut缩合、还原、水解六步反应制备含羟甲基结构的MQCA衍生物7-羟基甲基-3-甲基-喹噁啉-2-羧酸,为制备对游离残留标示物高亲和力的抗体,高灵敏度的ELISA残留检测方法的建立奠定基础。

In the thesis, a series of novel single and multi-armed oxadiazole bridging ligands and pyridyl-4, 5-imidazole dicarboxylic acid ligands have been synthesized. The coordination chemistry of these novel ligands with transition metal ions was investigated. In addition, two 1, 3, 4-oxadiazole bridging ligands end-capped by chelating Schiff-base coordination sites have been designed and synthesized. The coordination chemistry based on one of the two ligands with metal ions was primarily investigated.

本文报道了一系列新颖的含1, 3, 4-Oxadiazole杂环的单臂及多臂有机配体,研究了它们与过渡金属离子的配位反应化学;合成了吡啶基-4,5-咪唑二羧酸有机配体,研究了它们在水热条件下与过渡金属离子的配位反应化学;另外,还合成了1, 3, 4-Oxadiazloe杂环桥联的双Schiff-base有机配体,初步探讨了其与过渡金属离子的配位反应化学。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。