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Methods: The multinucleated osteoclasts were derived by 1,25-dihydroxyvitamin D3 and dexamethasone from bone marrow cells in the coculture system with primary osteoblastic cells.

采用原代培养的成骨细胞和骨髓单核细胞联合培养的方法,在1.25-2维生素D3和地塞米松作用下,使骨髓单核细胞分化形成破骨细胞。

PCL could be separated into anterolateral bundle and posteromedial bundle and there were "functional bundles" in each of them, which guaranteed the rearward stability of tibia in normal range of motion of the knee.

前外侧束与后内侧束中均存在&功能束&,两者的&功能束&联合作用可基本维持膝关节活动范围内胫骨的后向稳定性,&功能束&止点与两束止点中心不吻合。

Toxicity of abamectin and rubble seed oil against Tetranychus cinnabarinus and joint action of their mixture were evaluated in the lab.

室内测定了阿维菌素和橡胶籽油对朱砂叶螨的毒力及其复配剂的联合作用。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization-embryo transfer program and were cultured with M199 medium plus various concentration of leptin (0, 10, 30, 100, 300 ng/ml),human menopausal gonadotropin (hMG, 0, 0.1, 0.2, 0.5, 1, 2, 5, 10 IU/ml) and testosterone 100 μg/ml. For 2 days the media were collected for estradiol and progesterone measurements.

方法将来自体外受精-胚胎移植的卵巢黄素化颗粒细胞纯化后,在不同浓度瘦素(0、10、30、100、300 ng/ml)和人绝经期促性腺激素(hMG,0、0.1、0.2、0.5、1、2、5、10 IU/ml)单独或联合作用下进行体外培养,收集培养液,采用放射免疫方法测定颗粒细胞产生雌二醇及孕酮的量。

In order to study the influences of different wind speeds and wind yawed angles on the buffeting performance of bridge tower during construction,a wind tunnel test of bridge tower aeroelastic model was made based on considering the action of tower crane.

为了研究不同风速、风偏角在施工阶段对桥塔抖振性能的影响,进行了考虑塔吊共同作用的桥塔联合气弹模型风洞试验。

The combined effect of environmental endocrine disruptors is one of the hottest topics.

环境内分泌干扰物的联合作用已经是目前研究的重点。

In the damage zone parameters of rock mechanics get worse because of the actions of blast stress wave and explosion product,and this may produce potential dangers to the preserved rock and the constructions built on it.

在该区内,爆炸应力波和爆生气体的联合作用使得岩石力学参数劣化,给岩体及其岩体建筑物的安全运行留下了隐患。

Synoviocytes were incubated with different concentration of methotrexate and total glucosides of paeony respectively or combined.

利用mtt比色法测定不同浓度的甲氨蝶呤和白芍总苷单独或联合作用于滑膜细胞,对滑膜细胞增殖的影响。

We find that,because of the strong spatial anisotropy of theelectron hoppings,the on-site Coulomb interaction has different effects on these magnetic orderstates.

此外,我们发现是格点和最近邻库仑相互作用的联合作用使得CE相变成相对稳定的相。

Results:Total glucosides of paeony in concentration of 0.8~25.6 mg/L demonstrated marked dose-dependent inhibitory effect on fibroblast- like synoviocytes proliferation.

利用MTT比色法测定不同浓度的甲氨蝶呤和白芍总苷单独或联合作用于滑膜细胞,对滑膜细胞增殖的影响。

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