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As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP+Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组,以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组,以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组,作用时间为48小时,以不加药物干预作为对照组,Hoechst33258染色荧光显微镜下观察细胞形态的变化,流式细胞仪检测细胞凋亡峰;紫外光分光光度计检测线粒体PTP开放情况,流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化,Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性,γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

ConclusionAzone with different concentration and bornel inhibit each other when they are combinedly used.The absorption rate is maximum when azone is 0%.

冰片与不同浓度的氮酮联合使用时,氮酮与冰片有一定的相互抑制作用,氮酮浓度为0%时,有最大的透皮吸收速率常数。

Objective To study the efficacy and mechanism of applied microwave with traditional Chinese medicine and interferon therapy in the treatments of condylome acuminata.

摘 要]目的:探讨微波联合免疫疗法及中药治疗尖锐湿疣的疗效及作用机制。

Objective To study the efficacy and mechanism of applied microwave with traditional Chinese medicine and interferon therapy in the treatments of condylome acuminata.

广告载入中。。。广告载入中。。。尖锐湿疣;微波;干扰素;中药[摘要]目的:探讨微波联合免疫疗法及中药治疗尖锐湿疣的疗效及作用机制。

Objective To confirm the efficacy and safety of vaginally applied CO2 laser combined interferon therapy to treat female patients with condylome acuminata.

目的评估免疫疗法联合二氧化碳(CO2)激光治疗生殖道尖锐湿疣的疗效和作用机理。

Objective To study the efficacy and mechanism of applied microwave with traditional Chinese medicine and interferon therapy in the treatments of condylome acuminata.

来源:中国论文下载中心 [ 07-11-29 16:19:00 ] 作者:杨淑欣,刘明松编辑:studa20 尖锐湿疣;微波;干扰素;中药[摘要]目的:探讨微波联合免疫疗法及中药治疗尖锐湿疣的疗效及作用机制。

Evaluate the effects of B7-1 molecule combined with IFN-r /IL-12 in the primary cytolytic activity against HHCC.

评价B7-1分子联合细胞因子在特异性抗肿瘤免疫中的作用。

Combined Anisodine and DAIDLE shows significant effects on cardioprotection in swine experimental study.

联合应用DADLE和山莨菪碱能产生较好的心肌保护作用。

Objective To evaluate the therapeutic effect of naevus fuscocaeruleus zygomaticus treated by using Q-switched Nd:YAG laser associated with recombinant human epidermal growth factor to lessen pigmentation and decurtate course of treatment.

摘要]目的:评价Q开关Nd:YAG激光联合重组人表皮生长因子(recombinant human epidermal growth factor,rhEGF)在治疗颧部褐青色痣(naevus fuscocaeruleus zygomaticus NFZ)过程中对减轻色素沉着,缩短疗程的作用。

objectiveto evaluate the therapeutic effect of naevus fuscocaeruleus zygomaticus treated by using q-switched nd:yag laser associated with recombinant human epidermal growth factor to lessen pigmentation and decurtate course of treatment.

摘要]目的:评价q开关nd:yag激光联合重组人表皮生长因子(recombinant human epidermal growth factor,rhegf)在治疗颧部褐青色痣(naevus fuscocaeruleus zygomaticus nfz)过程中对减轻色素沉着,缩短疗程的作用。

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