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The synergistic action of ALOX5AP gene and IL-1A gene for IS was analysed by UNPHASED 3.0.7 version software.

用UNPHASED软件分析ALOX5AP基因与IL-1A基因对IS的联合作用

Methods The method of PCR- RFLP was conducted to examine the genotype of 3 cPLA2 genes ( PLA2G4A, PLA2G4B and PLA2G4C genes) in 263 subjects, including 121 cases of T2DM and 142 controls. The conditional test was used to test the combined effect of distinct loci on the T2DM by conditioning on allele or by conditioning on genotype with UNPHASED analysis platform. Results The genotypic frequencies of the 3 genes did not deviate from Hardy-weinberg equilibrium in both case and control groups.

采用聚合酶链反应-限制性内切酶片段长度多态性方法检测T2DM患者(病例组121例)和健康对照(对照组142例)3个cPLA2基因(PLA2G4A、PLA2G4B和PLA2G4C基因)的基因型;借助UNPHASED分析平台,利用等位基因条件分析和基因型条件分析方法分析基因的联合作用与T2DM的遗传相关性。

The HardyWeinberg equilibrium for genotypic distribution was estimated by the goodnessoffit test.The UNPHASED program was applied to perform the transmission disequilibrium test and the combined effect analysis of the paired SNPs.

利用拟合优度χ2检验分析等位基因和基因型分布频率是否符合哈迪-温伯格定律,应用遗传统计学软件进行2个SNPs的传递不平衡检验以及双位点联合作用分析。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization-embryo transfer program and were cultured with M199 medium plus various concentration of leptin (0, 10, 30, 100, 300 ng/ml),human menopausal gonadotropin (hMG, 0, 0.1, 0.2, 0.5, 1, 2, 5, 10 IU/ml) and testosterone 100 μg/ml. For 2 days the media were collected for estradiol and progesterone measurements.

方法将来自体外受精-胚胎移植的卵巢黄素化颗粒细胞纯化后,在不同浓度瘦素(0、10、30、100、300 ng/ml)和人绝经期促性腺激素(hMG,0、0.1、0.2、0.5、1、2、5、10 IU/ml)单独或联合作用下进行体外培养,收集培养液,采用放射免疫方法测定颗粒细胞产生雌二醇及孕酮的量。

Then nonlinear constructive relationship and failure criterion combined with 3D dynamical finite element analysis is applied to the arch darn and abutment considering static loads and seismic loads.

采用非线性本构模型和破坏准则,结合三维动力有限元法对江坪河高拱坝在静荷载和地震联合作用下的响应进行非线性分析。

The construction method of concreting layer by layer and segment by segment could reduce the use of steel and save construction cost,when concrete box arch ring was built cast-in-place with aid of arch centering.

混凝土箱型拱桥在无支墩拱式拱架上现浇施工时,采用分环分段的施工方法,可以利用拱圈与拱架的联合作用,减少钢拱架的用钢量,达到节省施工成本的目的。

After cocultured with PbCl2 and Na2SeO3 respectively and simultaneously,the growth of neurites and survival of neurons were tested.

建立新生Wistar大鼠海马神经元原代无血清培养技术,通过PbCl2,Na2SeO3单独及联合作用,观察神经细胞的生长和存活情况。

The combined effect of environmental endocrine disruptors is one of the hottest topics.

环境内分泌干扰物的联合作用已经是目前研究的重点。

In the damage zone parameters of rock mechanics get worse because of the actions of blast stress wave and explosion product,and this may produce potential dangers to the preserved rock and the constructions built on it.

在该区内,爆炸应力波和爆生气体的联合作用使得岩石力学参数劣化,给岩体及其岩体建筑物的安全运行留下了隐患。

Synoviocytes were incubated with different concentration of methotrexate and total glucosides of paeony respectively or combined.

利用mtt比色法测定不同浓度的甲氨蝶呤和白芍总苷单独或联合作用于滑膜细胞,对滑膜细胞增殖的影响。

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