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Methods: Adopting pig bile culture medium, the inhibiting or killing effects on pig bladder worm in vitro were observed;by using the methods of xylene-induced ear inflammation in mice and granuloma swelling induced by cotton ball in rats, the effects of anti-inflammation were observed;by using the methods of body twisting and hot-plate in mice, the effects of analgesia were observed. Through the mice auricle microcycle, the rats thrombus law in vitro and bleeding time, clotting time determination to the mice, the function of invigorating the blood were observed;adopting the penicillin abdominal cavity injection method, the mice epilepsy model were prepared, and epilepsy mice brain organization MDA content and SOD, ATPase vigor be measured by using chemical colorimetry.

利用猪胆汁培养基观察该方在体外对猪囊尾蚴的抑制或杀灭作用;采用二甲苯所致小鼠耳肿胀法、大鼠棉球肉芽肿法以观察该方的抗炎作用;采用热板法和化学刺激扭体法观察该方的镇痛效果;通过小鼠耳廓微循环法、大鼠体外血栓法和对小鼠出、凝血时的测定观察该方的活血作用;采用青霉素腹腔注射法制备小鼠癫痫模型,并利用化学比色法检测癫痫小鼠脑组织中MDA的含量及SOD、ATPase的活力。

Toreniafournieri. has a naked embryo sac that protrudes from the micropyle into the cavity between the funiculus and placenta. As embryo sac is mature, the egg cell, the two synergids and about half the central cell are located outside the micropyle.

蓝猪耳在其发育的四核期,胚囊从珠孔端伸出,当胚囊完全成熟时,助细胞、卵细胞和大部分中央细胞均暴露于胚珠组织之外,是研究被子植物受精和胚囊发育的模式植物。

IUGR, tetraphocomelia, or hypomelia caused by mesomelic shortening of the limbs with radial defects and oligodactyly or syndactyly (the upper limbs are more severely affected than lower limbs), cleft lip/palate, large genitalia, congenital heart defects, cystic kidneys, characteristic face with hypertelorism, a prominent premaxilla, a mid-face capillary hemangioma, cloudy corneas or cataracts and dysplastic or small ears, micrognathia, beaked nose, ear malformations, and mental retardation

宫内生长受限,由于四肢中间缩短引起的四肢短小畸形或短肢畸形并桡骨缺陷和少指畸形或并指畸形(对上肢的影响比下肢更严重),唇腭裂,生殖器肥大,先天性心脏病,囊状肾,特征面容:眶距过宽、前额突出、脸中部毛细血管瘤,角膜混浊或白内障和小放的或发育不良的耳,小颌畸形,钩状鼻,耳畸形和精神障碍。

The principal components of the vestibular system are the semicircular canals and the utricle and saccule.

半规管是主要的旋转运动感受器,椭圆囊和球囊内有听斑,其中含有耳石。

Methods The otoconia of 18 guinea pigs were damaged by a high G force (10 Gy 5 min), and their utricle macula were observed by scanning electron microscopy at 1 h, 2 d and 8 d after the damage respectively. The elements of different kinds of otoconia were measured qualitatively and quantitatively by electron probe Xray microanalysis.

给予过载刺激(10 Gy 5 min)造成18只豚鼠耳石的损伤破坏,用扫描电镜(scanning electron microscopy,SEM)分别观察过载刺激后1 h、2 d、8 d时其椭圆囊囊斑,用X射线显微能谱分析技术(electron probe Xray microanalysis,EPMA)测量不同形态类别的耳石元素构成。

Methods 4 adult male cadaveric (8 sides) were dissected to study the position of the auriculotemporal nerve and masseteric nerve ...

结果所有耳颞神经干均有部分与关节囊或髁状颈部骨质直接接触,最长有14.6mm,最短为5.6mm;在关节囊内侧分支最多,平均为3.5支,而在关节囊前壁没有分支。

When electric fusion method was used for nuclear transfer, the fusion rate (46. 0%), cleavage rate (53. 9%) and blastocyte development rate (10.9%) of adult ear fibroblasts were significantly lower than that of fetal fibroblasts (64. 5%, 70.1%, 21. 6% respectively), fetal skin cells (71. 5%, 70.8%, 22. 1% respectively) and ovary granulosa cells (88. 2%, 79. 1%, 25. 5% respectively). There was no significant difference among other donor cells in the cleavage and blastocyst development rate of resconstituted embryos.

当用电融合法进行核移植时,成体耳部成纤维细胞的融合率(46.0%),卵裂率(53.9%)和囊胚发育率(10.9%)均显著低于胎儿成纤维细胞(64.5%,70.1%和21.6%),胎儿皮肤细胞(71.5%,70.8%和22.1%),以及卵巢颗粒细胞(88.2%,79.1%和25.5%);另外三种细胞间的卵裂率,囊胚发育率无显著差异,但卵巢颗粒细胞的融合率显著高于胎儿成纤维细胞和胎儿皮肤细胞(88.2%vs 64.4%,71.5%,P<0.05)。

Using single oocyst infected techniques, three strains of Eimeria tenella, PT0705, FQ0709 and LJ0711, were obtained from classically symptomic coccidian chicken in Putian, Fuqing and Lianjiang Couties, Fujian Province. In order to compare their toxicity against chicken, healthy chicken of 14th-day and 21th-day were artificially infected with 1×104, 5×104 and 10×104 coccidian oocysts of PT0705, FQ0709 and LJ0711, respectively. The results showed that the strain PT0705 was the most toxic to tested chicken based on the clinical symptom, survival rate, weight increment and lesion grade investigations.

采用单卵囊分离技术,从福建省莆田市、福清市和连江县等地鸡场采集的病鸡盲肠内容物样品中,分离获得3株(PT0705、FQ0709、LJ0711)柔嫩艾美耳球虫纯种;为比较这三种虫株对雏鸡的毒性,分别用1×104、5×104和10×104个孢子化卵囊的剂量感染14日龄和21日龄健康无球虫感染雏鸡,通过临床表现、死亡率、增重情况、病变计分等指标比较,发现PT0705虫株呈现最强毒性。

Objective To study the relationship of endangium proliferation and the expression of metalloproteinases and tissue inhibitors of metalloproteinases after angioplasty of iliac artery in rabbits to investigate the probable mechanism of composite Danshen pill in prevention and treatment of restenosisMethods 30 white male rabbits of Japan (the average body weight was 25~30 kg)were randomly divided into 3 groups:normal control group,model group (intima destroyed by balloon and given hypercholesterol diet),CDP group (intima destroyed by balloon and given hypercholesterol diet plus drug CDP 150 mg/d),10 ones each groupResults The results of iliac artery angiogram and the analysis of pathology:there were lumens stenosis,thinner intima,smaller intima area,in CDP group compared with those in model group,and ratio of intima and tunica media thickness and area among each group (P<001)Immunohistochemistry analysis:the MMP2,MMP9,TIMP1,TIMP2 in model group significantly increased than those in control group (P<001)The CDP group had lower MMP2 and MMP9 expression,higher TIMP1 and TIMP2 expression,and increased MMP2/TIMP1,MMP9/TIMP2 than model group (P<001)The thickening of vascular neointima and stenosis degree of vascular lumen were relevant to MMP2 and MMP9 (r=0896,P<001)Conclusions MMP and TIMP play the very important role in the restenosis process of arteryCDP could inhibit the thickness of vascular neointima after balloon injury,the probable mechanism of which may be inhibiting collagen formation,smooth muscle immigration and decreasing hyperplasia of intima by interfering expression of MMPs and TIMPs

目的 研究兔髂动脉成形术后血管内膜增生和基质金属蛋白酶及其抑制物表达之间的关系,探讨复方丹参滴丸预防再狭窄的可能机制。方法健康成年二级雄性日本大耳白兔30只,平均体重25~30 kg。随机分为3组:正常对照组10只,模型组10只(球囊内膜剥脱加高胆固醇饮食),治疗组10只(球囊内膜剥脱加高胆固醇饮食以及复方丹参滴丸150 mg/d)。结果兔髂动脉造影、血管病理图像分析检测结果:①复方丹参滴丸组较模型组血管造影示管腔直径狭窄、内膜厚度减少、内膜面积减少、内膜厚度和中膜厚度比、面积比,各组之间有显著性差异(P<001)。②免疫组化分析:模型组MMP2、MMP9、TIMP1、TIMP2表达均高于对照组(P<001);复方丹参滴丸组MMP2、MMP9的表达低于模型组,而TIMP1、TIMP2的表达高于模型组;TIMP1/MMP2、TIMP2/MMP9明显增加,差异有显著性(P<001)。③内膜的增生以及管腔的狭窄程度与MMP2、MMP9有很好的相关性(r=0896,P<001)。结论 MMP和TIMP在再狭窄形成中起重要作用;复方丹参滴丸能够明显抑制血管损伤后的内膜增生,可能的机制是通过影响金属蛋白酶MMP2、MMP9及其抑制物TIMP1、TIMP2的表达从而抑制胶原的生成、平滑肌的迁移、增生,而减少内膜的增生。

The results showed PERV proviral DNA and mRNA could be detected in all 31 individuals by PCR and RT-PCR, respectively. env-A, env-B and env-C were only detected in 2 individuals, while in the other 29 individuals, only env-A and env-B but not env-C was detected. The quantity of DNA in PCR amplification of PERV genes should be more than 15 ng.

PCR和RT-PCR结果表明,所检测的31头沙子岭猪均带有PERV前病毒DNA,耳样组织中均有PERV mRNA表达,其中有2头个体携带 env-A、env-B、env-C 3种囊膜蛋白基因,而其余的29头个体只带有env-A、env-B 两种囊膜蛋白基因,未检测到env-C基因。

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