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Methods: Retrospective study include 15 cases of auricle deformities during July 2003 to March 2008 was carried out.

选择2003年7月~2008年3月住院的耳廓缺损15例患者,其中先天性小耳畸形12例,外伤后耳廓缺损3例。

The results showed that mice in the photoallergy group pretreated with sulfanilamide and UV irradiation developed evident ear swelling at 24h after challenge and the histopathology was characteristic of DTH, while control mice showed no reaction, indicating that sulfanilamide is a kind of chemical with photoallergenicity.

结果显示,受试物对氨基苯磺酰胺仅在光变态反应组中于激发后24小时出现耳肿胀反应,耳组织学改变符合DTH的病理特征,在其余两个对照组中均不出现耳肿胀反应,证实为光变应性药物。

Part three:Cochlear on Hair Cell Like Cells Regeneration in Presbycusis C57BL/6 Mice after Math1 Gene Inner Ear TransfectionIn order to test the probability of Mathl gene induces hair cell like cells regeneration in cochlea of presbycusis C57BL/6 mice,8 mounths old C57BL/6 mice were used for this study and were microinjected with Ad-MathlorAd-EGFP in the scala tympani by ventral approach.

第三部分老年性聋模型小鼠C57BL/6耳蜗鼓阶外源基因Math1导入后出现毛细胞样细胞现象的观察为了观察老年性聋C57BL/6小鼠(8月龄)耳蜗鼓阶外源基因Math1导入后能否在老年性聋的耳蜗微环境诱导出新生的毛细胞样细胞,为Math1基因治疗老年性聋进行探索性研究。

Results: Because of the early reparation,53 auriculae of patients were alive again.The ratio of success was 82.8%.The temperature,sense and appearance of the auriculae were normal.9 cases of auriculae were alive partially.

结果:53例经修复及再植后完全成活,温度及感觉正常,外观良好,成活率为82.8%。9例部分成活,占全部患耳的14.1%。2例失败,遗留明显耳廓畸形,以后行Ⅱ期耳廓整形术,占全部患耳的3.1%。

So long as Hector lived and Achilles nursed his anger, and so long as the city of Priam remained untaken, the great wall of the Achaeans stood firm; but when the bravest of the Trojans were no more, and many also of the Argives, though some were yet left alive when, moreover, the city was sacked in the tenth year, and the Argives had gone back with their ships to their own country- then Neptune and Apollo took counsel to destroy the wall, and they turned on to it the streams of all the rivers from Mount Ida into the sea, Rhesus, Heptaporus, Caresus, Rhodius, Grenicus, Aesopus, and goodly Scamander, with Simois, where many a shield and helm had fallen, and many a hero of the race of demigods had bitten the dust.

只要赫克托耳仍然活着,阿基硫斯怒气不消,只要王者普里阿摩斯的城堡不被攻陷,阿开亚人的高墙就能稳稳当当地站立。但是,当所有最勇敢的特洛伊人战死疆场,众多的阿耳吉维人长眠客乡,剩下一些人回返后,当普里阿摩斯的城堡在第十个年头里被阿耳吉维人捣毁,后者驾着海船回返他们热爱的故乡后,那时,波塞冬和阿波罗议定,引来滚滚的河水,冲袭扫荡,捣毁护墙。

LAA mechanical activity prior to ECV is highly related to maintenance of sinus rhythm in NVAF, and LAA "stunning" developed a thrombogenic milieu. Patients with atrial fibrillation should be given full anticoagulation to prevent thrombogenesis in left atrial and LAA.

NVAF患者电转复前左心耳收缩功能与窦性心律的维持高度相关;转复后左心耳&顿抑&,出现了血栓易于形成的条件,故对此类患者电转复后应给予足够的抗凝治疗,预防左房与左心耳血栓形成。

Kanic acid was injected into guinea pigs' cochleae and the excitotoxicity model was established. After a week the recombinant plasmid was transferred into SGCs of guinea pigs' cochlea treated with HAT. The following week the expression of NT-3 was examined by the immunohistochemical method, and the morphology of SGNs was observed under the electronic microscope after 4 weeks, in the mean time the changes of auditory brain-stein response were examined.

通过耳蜗灌注海人酸(kainic acid, KA)建立豚鼠耳蜗兴奋性损伤模型,在给KA 1周后利用HAT携带重组质粒进行耳蜗灌注以转染耳蜗螺旋神经节细胞,免疫组化法观察转染后1周NT-3的表达及4周后电镜下螺旋神经节细胞形态学变化,同时观察对听觉脑干诱发电位(auditory brain-stem response, ABR)的影响。

Results In three Hank's solution infused cochleas, the thresholds of IC potential were kept in the normal level and no hair cell loss was found 14 days after the beginning of the infusion, suggested that the pump implantation and Hank's solution infusion had no significant effects on the cochlear functions. In contrast, threshold shifts of IC potentials and hair cell loss were found in two BSO infused animals, suggested that the infused solution had distributed into the whole cochlea.

结果 应用渗透压泵耳蜗灌流Hank液后,下丘电位阈值维持正常,耳蜗毛细胞无损伤;灌流BSO液14天后,所测频率(1、 2、 4和8 kHz)的下丘电位阈值平均升高28 dB,内耳毛细胞平均缺失39.3%,缺失分布于耳蜗各回,表明渗透压泵工作良好。

Effects of Bushen Pill on Apoptosis Genes of Spiral Ganglion Cells Guinea Pigs with OtotoxityWANG Feng, ZHAO WulanZhejiang Chinese Medical University, Hangzhou 310053, China Abstract:ObjectiveTo explore the protective effects of Jingui Shenqi Pill on apoptosis genes of spiral ganglion cells in guinea pigs with ototoxity by gentamycin; MethodsThe ototoxity model was induced by abdominal injection of 100 mg/ gentamycin,while the treatment group took Jinguishenqi pill after injection of gentamycin, executed after the experiment, and the cochleas were gathered for detection.

作者:王枫,赵乌兰作者单位:浙江中医药大学,浙江杭州 310053 目的探讨金匮肾气丸对庆大霉素造成药物性耳聋豚鼠耳蜗螺旋神经节细胞凋亡调控基因表达的影响。方法豚鼠腹腔注射庆大霉素100 mg/制作耳聋动物模型,治疗组在注射庆大霉素后灌服金匮肾气丸水煎剂,实验结束后豚鼠断头处死,取耳蜗标本。

Methods ①The spiral organ of corti of 10 mice (20 ears) were taken, and divided into 2 groups randomly (n=10), cultured in 0.1 mol·L-1 gentamicin and the metabolite of 0.1 mol·L-1 gentamicin for 48h, then the number of hair cells remain was recorded;②The spiral organ of corti of 10 mice were taken (20 ears), and divided into 2 groups randomly (n=10), 3% CaCl2 and 0.1 mol·L-1 GM were mixed in the culture medium and the number of hair cells remain in the two medium mentioned above were recorded;③The spiral organ of corti of 10 mice were taken (20 ears), divided into 2 groups randomly (n=10), 3% MgSO4 to 0.1 mol·L-1 GM were mixed in the culture medium, the number of hair cells remain in the two medium mentioned above were recorded.

①取10只小鼠的耳蜗螺旋器(20个),随机分为2组(n=10),分别在含0.1 mol·L-1 GM与0.1 mol·L-1GM代谢产物的培养液中培养48 h后,记数残留的耳蜗毛细胞数;②取10只小鼠的耳蜗螺旋器(20个),随机分为2组(n=10),分别在含0.1 mol·L-1 GM与0.1 mol·L-1GM+3% CaCl2的培养液中培养48 h后,记数残留的耳蜗毛细胞数;③取10只小鼠的耳蜗螺旋器(20个),随机分为2组(n=10),分别在含0.1 mol·L-1 GM与0.1 mol·L-1GM+3% MgSO4的培养液中培养48 h后,记数残留的耳蜗毛细胞数。

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