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Methods Using a combinatorial approach of enzymatic digestion and mechanical separation to allow isolation and culture of GER cells from P1 rat cochleae.

方法取生后1d大鼠耳蜗,利用机械分离和酶消化相结合的方法分离出纯GER细胞,分别于无血清和含10%胎牛血清的DMEM培基中进行培养,观察细胞生长特性,并对培养物进行溴脱氧尿苷、鬼笔环肽、Z01、钙视网膜蛋白及肌球蛋白VIIa免疫组化鉴定及扫描电镜观察。

The infused and the contralateral, non-infused cochleae were harvested and stained with X-Gal, the Ad-LacZ infected cochlea were stained with blue color.

我们将Ad-lacZ病毒注入豚鼠内耳中,分别在病毒注入二天、一周、二周后取材固定进行X-Gal染色可观察到,整个耳蜗从蜗底到蜗尖均被染成兰色。

Eight or ten months, then, after that which is related in the preceding pages, towards the first of January, 1823, on a snowy evening, one of these dandies, one of these unemployed, a "right thinker," for he wore a morillo, and was, moreover, warmly enveloped in one of those large cloaks which completed the fashionable costume in cold weather, was amusing himself by tormenting a creature who was prowling about in a ball-dress, with neck uncovered and flowers in her hair, in front of the officers' cafe.

在上面几页谈过的那些事发生后又过了八个月或十个月,在一八二三年一月的上旬,一次雪后的晚上,一个那样的佳公子,一个那种游民,一个&很有思想的人&,因为他戴了一顶莫里耳奥,此外还暖暖地加上一件当时用来补充时髦服装的大氅,正在调戏一个穿着跳舞服、敞着胸肩、头上戴着花、在军官咖啡馆的玻璃窗前来往徘徊着的人儿。

Methods: thirty-four Japanese white rabbits are used as experimental animals. Under no destroying the posterior ligaments, expose the fifth and sixth transverse processes and intertransverse membrane. The transverse processes are decorticated. On each side bone graft bed the following four kinds of grafts are put, they are: autologous iliac crest、allograft+rhBMP-2、xeograft+rhBMP-2、and xeograft+HA.

34只成年日本大耳白兔,在不破坏脊柱后侧稳定结构的前提下,显露双侧的L5、L6横突,用磨钻去皮质,然后每侧分别随机地植入2.5ml左右的下列四种植入物:自体髂骨、同种冷冻干燥异体骨+rhBMP-2、异种冷冻干燥骨+rhBMP-2、异种冷冻干燥骨+HA。

After healing the cripple, Paul and Barnabas found that they were being deified as Hermes and Zeus respectively.

在医好生来就瘸腿的人后,保罗与巴拿巴却发现他们被众人奉为宙斯与希耳米。

Methods: NG108-15 cells were cultured in vitro at 37℃in a humidified atmospherecontaining 5%CO_2. Aβ_(25~35) fragment was dissolved with deionized distilledwater and aged for 4 days at 37℃. The cells were exposured to 5μmol/LAβ_(25~35) for 24h to establish the cell model. Twelve rabbits were divided intotwo groups at random, and garaged with MWD or physiological saline controlrespectively for 7 days to collect two kinds of cerebrospinal fluid, theyare CSF from rabbits treated with MWD and CSF from rabbits treated withphysiological saline.

NG108-15细胞在37℃、体积分数为5%的CO_2培养箱中培养,Aβ_(25~35)溶解于双蒸水中,置于37℃培养箱中老化4d,使其寡聚,毒性增强,细胞被置于浓度为5μmol/L的Aβ_(25~35)中作用24h,建立起AD细胞模型。12只清洁级大耳白家兔购于华中科技大学同济医学院动物中心,将其随机分成两组,分别给与等量的加味温胆汤和生理盐水灌胃7d,末次给药后1h内用水合氯醛将给药组白兔麻醉,1.2ml/kg,无菌条件下用7号注射针头从枕骨大孔处垂直进针,抽取清亮含药脑脊液。

Couldn't wash my hair in the shower because of an ear operation; this shampoo, although wet, was very easy to control, lathered well and wiped off with an absorbent towel just as the label described.

有一次因为耳部手术不能在沐浴中洗头,这个洗发露,即使是湿的,非常容易控制,容易起泡,等泡沫出现后就用干爽吸收力好的毛巾将头发擦干净。

After 4 and 10 days,observe the effect of Ainsliaea fragrans Champ on EGF expressed of cervix mucosae of rats of different dose group,and analyzed its pathomorphology.

方法建立大鼠宫颈炎动物模型,分别于给药4天、10天后观察杏香兔耳风不同剂量组对大鼠宫颈粘膜表皮生长因子表达的影响,并进行病理组织学检查。

Chainsaws produce a level of noise that will affect unprotected ears after only 15 minutes of exposure, wear ear muffs or ear plugs to keep as much noise as possible out.

链产生噪音,影响血泪耳朵曝光后仅15分钟,戴耳塞或耳外伤使尽可能多的噪音。

Piglet coccidiosis is a parasitic protozoosis, causing serious diarrhea. Thedisease mainly results from Isospora suis parasitizing small intestine epithelial cell ofnursing and freshly weaned piglets. Its main pathogen is I. suis, and the infected pigletsat 1 or 2 weeks postnatal exhibit nonhemorragic yellow or white diarrhea, with a lowmortality, but often followed by a consecutive bacterial or viral diseases, which increasesmortality in piglets and causes great economic losses in pig farms.

哺乳仔猪球虫病是由猪等孢球虫和某些艾美耳属球虫寄生于哺乳期及断奶仔猪小肠上皮细胞引起的一种以腹泻为主要临床症状的寄生性原虫病,其主要病原是猪等孢球虫,表现为出生后第一周发生非出血性的黄色至白色腹泻,临床症状并无特别之处,受害仔猪并不中止吮乳,死亡率较低,但常常会伴发一些继发性疾病(比如由细菌、病毒引起的疾病),造成仔猪的死亡率升高,从而给许多猪场带来了较为严重的经济损失。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷

Nusa lembongan : Bali's sister island, coral and sand beaches, crystal clear water, surfing.

Nusa Dua :豪华度假村,冲浪和潜水,沙滩,水晶般晶莹剔透的水,网络冲浪。