耐药的

Along with the fluconazole solution density rise,the experimental two kind of strain various glucose density is higher,showe d the glucose consumption are less,takes the logarithmof the medicine de nsity,discovered the logarithm the medicine density and each glucose den sity presents the linear relations;Carries on the analysis comparison to under the fluconazole function two kind of strain linear relations,disc overed the relations of the two strains has the nonuniformity.3 Compare the fluconazole induction reaiatance SC5314 strain and sens itive strain compares,its difference gene expression mainly concentrates in:The code proteinase body and the protein hydroltyic enzyme gene,in the code sugar fat metabolism process is connected the protein gene,the cell cycle correlation gene,the duplication and the translation adjustme nt correlation gene,the stress response correlation gene,the line plast ochondria correlation gene,the cell wall function related gene.4 Candida albicans SC5314 induction resiatance strain was processed b y Xianglian solution,its expression change gene mainly is:Code stress re sponse family protein gene,biomembrane relevant gene,a code proteinase body gene race,code cell cycle related protein gene,duplication and tra nslation adjustment related protein gene.5 The clinical reaiatance strain Candida albicans was processed by Xi anglian solution,its expression change gene mainly is:Codes the hot sho ck protein gene,the serine/threonine protein activating enzyme gene,the proteinase body family gene,the regulation copies and translates the ge ne.

随着氟康唑药液的浓度上升，试验的两种菌株各孔葡萄糖浓度越高，说明葡萄糖消耗越少，经过药物浓度取对数后进行分析，发现取对数后的药物浓度和每孔中葡萄糖浓度者呈现线性关系；对氟康唑作用下的两种菌株的线性关系进行分析比较，发现对两种菌株作用具有不一致性。3氟康唑诱导的耐药SC5314菌株与诱导前的敏感株相比，其差异基因表达主要集中在：编码蛋白酶体及蛋白水解酶的基因，编码糖脂代谢过程中相关蛋白的基因，细胞周期相关基因，转录及翻译调节相关基因，应激反应相关基因，线粒体相关基因，细胞壁功能相关基因。4白念珠菌SC5314诱导耐药株经香莲外洗液作用后，其表达变化的基因主要是：编码应激反应家族蛋白的基因，生物膜相关性基因，编码蛋白酶体基因一族，编码细胞周期相关蛋白基因，转录及翻译调节的相关蛋白基因。5白念珠菌临床耐药菌株经香莲外洗液作用后，其表达变化的基因主要是：编码热休克蛋白基因，丝氨酸/苏氨酸蛋白激酶基因，蛋白酶体家族基因，调控转录及翻译基因。

The overexpression of CDR1, CDR2, and MDR1 is a common theme in azole antifungal resistance in C.

其中多药耐药基因CDR1、CDR2、MDR1的表达增加是唑类耐药主要机制，有研究表明CDR1、CDR2、MDR1的过度表达与转录调控因子有关，但具体机制不清。

multidrug resistance is considered to be the main obstacle to the effective chemotherapeutics, which has been investigated for years in the medical field.

多药耐药（multidrug resistence，mdr）是肿瘤化疗失败的主要原因，多年来学者一直致力于多药耐药机制的研究。

Furthermore, the results of the antibiotics sensitivity test of 38 isolates to 24 kinds antibiotics show that all isolates have presented antibiotic resistance disparately as well as multi-resistance. All isolates are sensitive to Cephalosporins antibiotics and Aminoglycosides antibiotics such as Amikacin, Cefazolin, Cefradine, Cefoperazone, and Ceftriaxone. Thirty-eight isolates are extremely resistant to Penicillins antibiotics, Lincomycins antibiotics, Tetracyclines antibiotics and Macrolides antibiotics such as Midecamycine, Lincomycin, Carbenicillin, Tetracycline, Amoxicillin and so on. Antibiotics sensitivity test provides guidance for antibiotic application and scientific foundation from poultry farms.

按世界卫生组织推荐的Kirby-Bauer菌液涂布法对38株大肠杆菌进行24种抗生素的药物敏感性试验，结果表明：38株大肠杆菌均有不同程度的耐药，而且均表现多重耐药；较为敏感的抗生素是头孢菌素类和氨基糖苷类抗生素，其中以阿米卡星、头孢唑啉、头孢拉定、头孢哌酮和头孢三嗪最为敏感；对青霉素类抗生素、林可胺类抗生素、四环素类抗生素和大环内酯类抗生素耐药严重，其中以麦迪霉素、林可霉素、氨苄青霉素、四环素和阿莫西林耐药性最为严重；药物敏感试验为保定、秦皇岛和北京三个地区养鸡业提供科学的用药指南，为药物防治提供科学依据。

With fewer side effects, the reversal efficacy of 20 μmol/L lobeline was 71.6% of the classical MDR reversal agent of verapamil at the same concentration. ConclusionLobeline can reverse the MDR of MCF-7/ADM cells by inhibiting the activity of P-glycoprotein.

洛贝林对乳腺癌多药耐药细胞株MCF-7/ADM的耐药性具有逆转作用，其机制主要为抑制细胞多药耐药蛋白P-gp的活性

Methods］ MTT assay was employed to test the lethal concentration 50 (IC50) of sensitive germline (SK-OV-3) and ovarian cancer cell subline induced by ADR (SK-OV-3/adr) treated by 4 drugs, resistance index and reverse multiple were calculated. The changes of drug content were observed by fluorescence microscope, the intracellular accumulation of ADR was evaluated by fluorospectrophotometry, the changes of mdr 1/P-gp resistance were observed respectively by RT-PCR and Western blot.

方法］MTT法检测药物对敏感细胞株（SK-OV-3）和诱导的耐药细胞株（SK-OV-3/adr）的半数致死浓度（IC50），计算耐药指数和加逆转剂后的逆转倍数，荧光显微镜观察不同时间细胞内的药物含量变化，荧光分光光度法测定细胞内ADR的含量，RT-PCR和Western blot法检测细胞耐药前后mdr1/P-gp的变化。

In order to understand the drug resistance mechanism of HIV-1 integrase mutually existed for multiple drug-resistant strains to the most potent IN inhibitors diketo acids, three S-1360-resistant HIV-1 strains were selected and molecular docking and molecular dynamics simulations were performed to obtain the inhibitor binding modes.

为了解DKAs引起的多种耐药株共有的耐药性机理，选择3种S-1360引起的IN耐药突变体，用分子对接和分子动力学模拟，研究了野生型和突变型IN与S-1360的结合模式，基于该结合模式探讨了3种耐药突变体所共有的耐药性机理。

Drug sensitivity tests showed that the resistance to gentamycin,levofloxacin,trimethoprim/sulfamethoxazole,rifampin,erythromycin,elindamycin and tetracycline were hish among MuH and MuL strains,and most of these strains were multi-drug resistant.All strains were susceptible to vaneomycin.

药物敏感性试验显示，MuH和MuL菌株对庆大霉素、左氧氟沙星、复方磺胺甲恶唑、利福平、红霉素、克林霉素、四环素的耐药率都较高，且呈多药耐药，但对万古霉素全部敏感。

objective to study the infection and sensitivity to antimicrobial agents of staphylococcus of bacterial prostatitis patients.methods the results of bacteriology and drug susceptibility of 421 patients,who were suffered from prostatitis,were analysed retrospectively.results in the 421 succus prostaticus samples,278 strains pathogenic microorganisms of 15 genera were isolated from the 273 succus prostaticus samples,and the major pathogenic microorganisms was staphylococcus aureus(57.2%) and coagulase negative staphylocous（17.3%.the drug resistance rate of staphylococcus to penicilling,erythromycin and albomycin was 88%-97%,and it was 33%-50% to fluoroquinolones.the drug resistance rate of staphylococcus to amikacin,cefazolin,nemycin,rifampicin and cefoperazone was lower than 30%,and they all appeared susceptible to vancomycin.conclusion staphylococcus is the main germ of bacterial prostatitis and it appears high drug resistance to usual antibacterials.staphylococcus is susceptible to vancomycin,amikacin,cefazolin,neomycin,rifampicin and cefoperazone.

目的 探讨细菌性前列腺炎患者葡萄球菌的感染状况及抗菌药物敏感性。方法回顾分析421例前列腺炎患者标本的细菌学和药物敏感性试验结果。结果 421例前列腺液标本，273例分离出细菌，共分离出15个种别共计278株病原菌，其中金黄色葡萄球菌占57.2%，凝固酶阴性葡萄球菌占17.3%；葡萄球菌对青霉素g、红霉素、白霉素耐药率为88%～97%，对氟喹诺酮类耐药率为33%～50%，对丁胺卡那霉素、头孢唑啉、新霉素、利福平和头孢哌酮耐药率均0%，对万古霉素全部显示敏感。结论葡萄球菌是细菌性前列腺炎的最常见病原菌，对临床常用抗菌药物具有较高的耐药性，葡萄球菌对万古霉素、丁胺卡那霉素、头孢唑啉、新霉素、利福平和头孢哌酮较为敏感。

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr，用双向凝胶电泳技术分离总蛋白，银染及胶体考马斯亮蓝染色，image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。结论两种显色方法受样品量影响较大，恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。