羧基化

A novel procedure for the extraction of lysozyme from egg-white using carbon nanotubes as a sorbent was developed.

建立了以羧基功能化的多壁碳纳米管作为吸附剂在线提取蛋清中溶菌酶的新方法。

The analysis of 1H NMR showed that the succinyl group was successfully conjugated to the amino group of chitosan molecule, and the degree of substitution of Suc-Chi values increased with increasing succinic anhydride/amino mole ratio or reducing the molecular weight of chitosan.

利用壳聚糖和琥珀酰酐反应合成了N-琥珀酰壳聚糖，1H NMR分析表明通过反应可在壳聚糖的侧氨基引入亲水性的羧基，增加琥珀酰酐／氨基的摩尔投料比或者选择分子质量较小的壳聚糖，有利于提高N-琥珀酰壳聚糖的琥珀酰化度。

XPS and FTIR spectroscopy analysis indicated that chemical bonds were formed between maleic rosin and wood flour through the esterification reaction of anhydride groups and hydroxyl groups on wood surface.

FTIR与XPS分析表明，马来松香分子中的酸酐基团与木粉表面的羟基发生了酯化反应，并且马来松香是以单酯的形式接枝到木粉表面，同时产生－游离羧基。

After the diazotization ,2-aminophenol-4-(2-'carboxy)sulfanil phenylamine was coupled with acetoacetanilide,2-naphthol,1-phenyl-3-methyl-5-pyrazolone,1-naphthol-4-sulfonic acid,separately to get the different diazotized compounds,then complexed with potassium chromium sulfate or cobaltous chloride to obtain the yellow,orange,red,purple,blue and other colour dyestuffs.

2-氨基苯酚-4-(2'-羧基)磺酰苯胺重氮化，分别与乙酰乙酰苯胺、2—萘酚、1-苯基-3-甲基-5-吡唑啉酮、尼文酸、1-乙酰氨基-7-萘酚等偶合，得到不同的偶氮化合物，然后分别与硫酸铬或氯化钴进行络合反应，得到黄、橙、红、紫、蓝多种颜色染料，并进行了毛织品的染色实验。

Polyvinyl alcohol amidoxime chelate fiber was synthesised by pre irradiation graft copolymerization and amidoximation.

利用60Co－γ射线预辐照接枝和胺肟化反应合成了含羧基的聚乙烯醇胺肟螯合纤维。

AIM: To investigate the effect of PD98059, a specific repressor of extracellular signal regulated kinase1/2 (ERK1/2), on the proliferation of the murine lymphocytes. METHODS: Lymphocytes were isolated from lymphoid nodes of BALB/c mouse. After stained with carboxy fluorescein diacetate, succinimidyl ester, the cells were stimulated with polyclonal activators, concanavalin and phorbol 12,13 dibutyrate plus ionomycin.

分离小鼠淋巴结细胞，藉羧基荧光素乙酰乙酸染色，用多克隆刺激剂刀豆A蛋白或者佛波醇酯加离子霉素刺激，以流式细胞术分析PD98059对淋巴细胞增殖的影响；利用碘化丙锭染色分析PD98059对细胞周期的影响。

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞，建立小鼠T淋巴细胞活化、增殖的模型，以不同浓度的CPT作用于该模型，流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以Con A作为T细胞活化、增殖的刺激剂，以不同浓度的TMB-8及与环孢菌素A联合作用于该系统，用流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术，分析TMB-8在Con A刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色分析细胞周期的分布情况。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型，通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用；采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用；利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

Two coordination polymers composed of in situ synthesized ligand deriving from isophthalic acid through the hydroxylation on the 2-position have been synthesized under hydrothermal conditions.

利用铜离子的氧化还原性质，以铜离子和间苯二甲酸在水热条件下反应使配体发生羟基化衍生成2，6-二羧基苯酚，并组装出2个含衍生配体的配位聚合物。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。