羧基化

The regulative sequence of ribulose-1 ,5-bisphosphate carboxylase small subunit genes of rice was cloned by PCR amplification.

利用PCR法克隆了水稻日本晴来源的核酮糖-1、5-二磷酸羧化酶小亚基基因5'上游调控区，命名为Posrbcs。

In consonance withthe increase in the carboxylation efficiency,RuBPCase activity and relative contentof Rubisco large subunit were also increased by GA3 treatment.

GA3也可以提高RuBP羧化酶的活性和Rubisco大亚基的含量。

From the reaction mechanism, which involved formation of benzodiazepinium ethoxycarbonylmethylate, the reaction mechanism, which involved formation of benzodiazepinium ethoxycarbonyl- methylate followed by cycloaddition reaction, was tentatively proposed.

通过研究反应过程中分离出的副产物反丁烯酸二乙酯III和4，5-二氢吡唑-3，4，5-三羧酸乙酯IV，初步提出了反应经过乙氧羰基甲基化苯并二氮杂卓翁中间体V再发生环加成反应的机制。

The authors discuss elemental compositions and properties of SPH - 1 water-base graphite coating with sodium bentonite and carboxy methyl cellulose as thickner and graphite, soapstone powder and silica powder as basic constituents.

本文讨论了以钠基膨润土和羧甲基纤维素钠作为稠化剂，石墨、滑石粉、石英粉并用的SPH-1型水基石墨涂料的基本组成和性能。

According to the result, we believed that the reason resulted in the low sensitivity was that the marker residue compound MQCA structure has changed after conjugated with protein and the antibody induced by the conjugation could not recognize the free MQCA in the ci-ELISA. Therefore we designed some hapten which could reserve the marker residue structural speciality mostly. After synthesis route selection and optimum, the hapten 7-Hydroxymethyl-3-methyl-quinoxaline-2-carboxylic acid was synthesized, used 4-methyl-2-nitroaniline as the start material via six steps, such as oxidation cyclization, bromize reaction, hydrolysis, Beirut reaction, reduction, hydrolysis. This work established basis for producing the high affinity antibody and developing the sensitive ELISA residue detection method.

本论文认为导致ELISA检测灵敏度较低的原因，是残留标示物和蛋白偶联后的特征结构发生改变，诱导产生的抗体不能够对游离MQCA很好的识别，因此，本论文设计了能够最大化保留MQCA结构特征的系列半抗原结构，并对其合成路线进行了筛选和优化，最终以对甲基邻硝基苯胺为起始原料，经氧化环化、溴代、水解、Beirut缩合、还原、水解六步反应制备含羟甲基结构的MQCA衍生物7-羟基甲基-3-甲基-喹噁啉-2-羧酸，为制备对游离残留标示物高亲和力的抗体，高灵敏度的ELISA残留检测方法的建立奠定基础。

The stereochemical effect of the chiral amino acid residues on the formation of penta-coordinate phosphorus intermolecular mixed carboxylic-phosphoric anhydride from N-phosphorylamino acids was studied with density functional theory calculations at B3LYP/6-311G level.

用密度泛函方法在B3LYP/6-311G水平上对N-磷酰化氨基酸生成五配位磷酸羧酸混酐反应中手性氨基酸残基侧链的立体化学效应进行了研究。

In this research, the complete ipt gene was cloned from Agrobacterium pTiAch 58 Hind Ⅲ fragment and its promoter was re-constructed so that following three chimaeric genes were created:(1) ipt promoter plus ipt coding region and terminator ,(2) RuBP SSU 301 promoter from petunia plus ipt coding region and terminator ,(3) pea seed-specific vicilin promoter plus ipt coding region and terminator .

将ipt基因克隆后对其启动子进行了改造，分别构建了如下三种基因：（1）ipt启动子+ipt编码区和3′区，（2）磷酸核酮糖羧化酶小亚基启动子SSU 301+ipt编码区和3′区，（3）豌豆种子特异性启动子vicilin+ipt编码区和3′区。

Organic our country high polymer flocculant already has commercialized at present having several breeds such as PAM , polypropylene acidamides and carboxyl methyl cellulose only , studies innocuous high-effect organic synthesis high polymer flocculant (the ammonium equality gathers the sum copolymer kind if alkyl alkene propyl group halogenates waiting), optimizes it's the cost reduction composing handicraft.

目前我国有机高分子絮凝剂已商品化的仅有PAM、聚丙烯酰胺和羧甲基纤维素等几个品种，研究高效无毒的有机合成高分子絮凝剂(如烷基烯丙基卤化铵的均聚和共聚物类等)，优化其合成工艺降低成本。

The feasibility of electrocarboxylation of benzyl chloride has been investigated at silver cathode in CO2-saturated room-temperature ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate (BMIMBF4) solution for the first time.

在饱和了CO2的室温离子液体中，首次在银电极上研究了苄基氯电羧化的可行性。

The intermediate 5-amino-1H-pyrazole-4-carboxylic acid ethyl ester prepared in the first part underwent successively hydrazinolysis, condensations with ketene dithioacetals and alkylations to give rise to the dipyrazolyhnethanes.

从第一部分中的中间体5-氨基-1H-吡唑-4-羧酸乙酯开始，经过肼解、与乙烯酮二硫代缩醛的缩合反应和烷基化反应，合成了二吡唑基甲酮类化合物。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。