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羟脯氨酸

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In vivo experimental results revealed that PAP 10 and 20 mg.kg-1 could promote healing of radius fracture,accelerate osteotylus formation and increase contents of calcium and oxyproline.

整体鹿茸多肽能明显加速骨痂的形成及骨折的愈合,明显增加骨痂内羟脯氨酸和钙含量。

The CFb in blank control group was added with IMDM containing 10% FBS and those in Ang Ⅱcontrol group with Ang Ⅱ.The CFb in the 3 test groups were induced with Ang II,then added with Tau at dosages of 40,80 and 160 mmol/L respectively. Determine the proliferation of CFb by MTT method, the collagen content by oxyproline kit, the transforming growth factor- TGF-β1 (TGF TGF-β1) content by ELISA, the cell cycle by flow cytometer, the CFb NO content by nitrate reductase method, the iNOS activity by spectrophotometry, and the expression of iNOS protein by IFA.

胰酶消化法分离培养新生大鼠CFb,用Ang Ⅱ诱导促进其增殖,采用MTT法检测细胞增殖;羟脯氨酸试剂盒检测胶原含量;ELISA法测定转化生长因子β1(TGF-β1)蛋白的含量;流式细胞仪检测细胞周期;硝酸还原酶法、分光光度法和免疫荧光法检测CFb NO含量、一氧化氮合酶活性和iNOS蛋白的表达。

At the fifth day after BTH, SA and SiO2 spraying, seedlings were inoculated with Sphaerotheca fuliginea, and disease indexes, leaf lignin and HRGP contents were determined for four times at alternate days.

以抗白粉病甜瓜品种'银帝'和感病品种'卡拉克赛'为材料,用BTH、SA和SiO2溶液分别预处理,5 d后接种白粉病菌并分4次调查处理植株的发病情况、测定叶片细胞壁中木质素和羟脯氨酸糖蛋白含量。

4The Masson trichrome stain can be used for an important method for the observation of the fibrostic cells, The changes of the mast cell could be considered as an assist marker for the observation of the liver fibrogenesis.In addition the concentration of the hepatic hydroxyproline could be used for diagnosis of the hepatic fibrosis. clinical and experimental study.

Masson三色染色可以作为肝纤维化肝硬化组织细胞观察的一种重要方法:肝纤维化过程中,肥大细胞数量的动态变化,可以作为观察肝纤维化变化的一项辅助指标;肝脏羟脯氨酸的浓度变化可以作为肝纤维化的辅助诊断及应用于相关实验的研究。

Six rats of each group were randomly sacrificed on day 7,14,28 and 42 respectively.The histological changes of lung tissue were studied by HE and Masson's trichrome staining.Hydroxyproline level in lung tissue was measured by digestion method.Protein and mRNA expression of transforming growth factor-β1(TGF-β1) were assayed by immunohistochemistry and RT-PCR respectively.

各组分别于造模后第7、14、28及42 d随机处死6只,取肺组织行HE、Masson染色评价肺组织病理变化,消化法测定肺组织羟脯氨酸含量,分别采用RT-PCR和免疫组化法测定肺组织转化生长因子β1(TGF-β1)的mRNA和蛋白表达。

Then the mean pulmonary arterial pressure, the ratio of right ventricle weight to left ventric...

测定各组大鼠的平均肺动脉压、右心室和左心室+室间隔的重量比、右心室组织的蛋白和羟脯氨酸含量,并观察右心室和肺的病理形态。

The sutured tendons were observed withnaked eyes ,under a light microscope and a transmission electron microscope.

术后1周、2周、4周对肌腱断端缝合部行大体标本观察、光镜和透射电镜观察及羟脯氨酸含量测定。

At the end of the experiment, the first incisors of 1 , II , III group were pulled out respectively, some were decalcified for SEM and TEM examinants and HYP analyses; the others were not decalcified for SEM examinants.

实验结束时,取Ⅰ、Ⅱ、Ⅲ组的每只羊的第一对切齿,不脱钙扫描电镜观察牙釉质与牙基质的结构情况;脱钙后扫描和透射电镜观察牙基质结构,化学分析法分析牙基质中羟脯氨酸的含量。

RESULTS: Wound healing rate, wound healing time, histopathology analysis, quantity assay of macrophage, determination of hydroxyproline, proliferation of cell, assay of DNA contents and circle of cells, level of transforming growth factor-alpha, levels of interleukin-1, interleukin-6 and tumor necrosis factor, assay of keratinocyte collagenase-1, level of fibroblast growth factor receptor-1, level of monocyte chemoattractant protein-1 and level of keratinocyte plasminogen activator inhabitor type 2 were selected as the evaluation criteria of wound healing.

结果 筛选了创面愈合率、创面愈合时间、组织病理学分析、巨噬细胞定量分析、羟脯氨酸含量测定、细胞增殖情况、细胞DNA含量和细胞周期分析、转化生长因子-α水平、白细胞介素-1、白细胞介素-6和肿瘤坏死因子水平、角质细胞胶原酶-1含量测定、成纤维细胞生长因子受体-1水平、单核细胞化学诱导蛋白-1水平和角质细胞纤溶酶原活化抑制剂-2水平等十三种创面愈合评价指标。

Methods: using number one fumigate-washing prescription, jieeryin, PP powder and physiologic saline cure respectively the wound of the experiment Jimpy mice which have suffered form anus operation. Then we observed the bacterial number of granulation tissue, the content of hydroxyproline, and the emerging of granulation tissue at the fifth day after operation, and the change of granulation tissue at the seventh and fourteenth days after operation.

以熏洗一号、洁尔阴、PP粉、生理盐水分别治疗小鼠实验性肛旁手术伤口,观察各组小鼠伤口肉芽组织中细菌数、羟脯氨酸量、术后第5天肉芽组织出现情况、7和14天肉芽组织肉眼及病理学变化。

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