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羟脯氨酸

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Objective: To assess the effect of marrow stroma cell transplantation on constitution hydroxyproline of myocardial infarction in rabbit.

目的:评价骨髓基质细胞移植对组织羟脯氨酸含量的影响,探讨细胞移植对心肌梗塞后心室重塑机制。

Methods A total of 64 round wounds (diameter of 6 mm each) with total skin loss were made on the ventral side of rabbit ear using a trephine. Morphology and collagen metabolism of scar wounds were studied at 14,21,35,70 and 98 days after operation, respectively.

用圆形钻刀在兔耳腹面产生直径6 mm的全层皮肤缺损创面64个,分别在术后14、21、35、70和98 d进行形态学观察与羟脯氨酸含量测定。

The alveolitis and pulmonary fibrosis were examined by HE staining. The serum HYP was examined .The expression of CTGF was tested by immunohistochemical assay.

HE染色检测肺组织肺泡炎及肺纤维化程度;测定血清羟脯氨酸的含量;免疫组化染色检测CTGF的表达。

Objective After Intense pulsed light irradiation cavia cobaya skin, to evaluate the histological change of skin with HE stains; to evaluate the collagen fiber change with nitroxanthic acid stains; to quantitative analysis of hydroxyproline content. Confirm mechanism of action for Intense pulsed light on sun-damaged human skin.

目的 用强脉冲光照射豚鼠背部皮肤后,通过HE染色观察皮肤组织学的改变;用苦味酸染色观察皮肤胶原纤维的改变;定量分析豚鼠皮肤羟脯氨酸的含量,观察皮肤胶原蛋白的变化,进一步研究强脉冲光治疗皮肤光老化的作用机制。

Methods 60 female mice were randomly divided into 4 groups (n=15):Mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of D-galactose[1000 mg/(kg1·d)] for 42 d to establish subacute aging model.

60只雌性昆明种小鼠,随机分为4组(n=15):正常对照组皮下注射生理盐水;其余3组每日颈背部皮下注射 D-半乳糖[1000 mg/] 造成小鼠衰老模型,同时各组分别灌胃生理盐水、枸杞多糖[200 mg/]、丹参酮[1 500 mg/]。42 d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

objective to compare the anti-aging effects of lycium barbarum polysaccharides and tanshinone on the skin of d-galactose-induced mice aging model.methods 60 female mice were randomly divided into 4 groups (n=15):mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of d-galactose[1000 mg/(kg1·d)] for 42 d to establish subacute aging model.the experimental groups included agingmodel group,lycium barbarum polysaccharides [200 mg/group,tanshinone [1 500 mg/] group.forty two days later,the dorsal skin samples were collected to determine sod activities、mda and hydroxyproline contents.

目的 比较枸杞多糖与丹参酮对衰老小鼠皮肤的抗衰老作用。方法 60只雌性昆明种小鼠,随机分为4组(n=15):正常对照组皮下注射生理盐水;其余3组每日颈背部皮下注射 d-半乳糖[1000 mg/]造成小鼠衰老模型,同时各组分别灌胃生理盐水、枸杞多糖[200 mg/]、丹参酮[1 500 mg/]。42 d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

objective to compare the anti-aging effects of lycium barbarum polysaccharides and tanshinone on the skin of d-galactose-induced mice aging model.methods 60 female mice were randomly divided into 4 groups (n=15):mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of d-galactose[1000 mg/(kg1·d)] for 42 d to establish subacute aging model.the experimental groups included agingmodel group,lycium barbarum polysaccharides [200 mg/group,tanshinone [1 500 mg/] group.forty two days later,the dorsal skin samples were collected to determine sod activities、mda and hydroxyproline contents.

60只雌性昆明种小鼠,随机分为4组(n=15):正常对照组皮下注射生理盐水;其余3组每日颈背部皮下注射 d-半乳糖[1000 mg/]造成小鼠衰老模型,同时各组分别灌胃生理盐水、枸杞多糖[200 mg/]、丹参酮[1 500 mg/]。42 d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

3 The effects of APS and AstragalosideⅣon synthesis of collagen Measure the Radix Astragali impact on NIH3T3 cells collagenic synthesis of many candies with the hydroxyproline reagent box.

2.3黄芪多糖和黄芪甲苷对胶原合成的影响本实验以NIH3T3为细胞模型,用羟脯氨酸试剂盒测量黄芪多糖对NIH3T3细胞胶原合成作用的影响。

A simple, rapid and accurate method for the determination of hydroxyproline in collagenous proteins by high performance liquid chromatography-electrospray mass spectrometry (HPLC-ESI/MS) was developed.

建立了一种简单、快速地测定胶原蛋白中羟脯氨酸的高效液相色谱-电喷雾质谱(HPLC-ESI/MS)方法。

The dags in experimental group were performed with anastomosis of intestine by using medical adhesive,while the dogs in control group were treated by using traditional modus operandi.

将分别测定两种吻合方法的操作时间、吻合口通畅程度、吻合口爆裂压、吻合口羟脯氨酸含量及标本组织病理学比较。

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