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Results: The untreated cells were spindle-shaped adherent cells.

在镜下,观察MSCs在诱导前后的形态变化;用免疫荧光染色检测酪氨酸羟化酶的表达。

Objective: To assess the effect of marrow stroma cell transplantation on constitution hydroxyproline of myocardial infarction in rabbit.

目的:评价骨髓基质细胞移植对组织羟脯氨酸含量的影响,探讨细胞移植对心肌梗塞后心室重塑机制。

Extensive experimentation revealed several issues, along with several corresponding solutions:(1) given that lithium metal dissolves slowly in liquid ammonia, considerable time was required to generate the dissolved metal solution (ca. 1 h);(2) the t -BuOH must be freshly distilled prior to use 21 to diminish the formation of ketone 19 (Figure 2);(3) efficient capture of the lithium enolate ( 11 ) required the use of freshly distilled TMSCl and Et 3 N; and (4) the presence of residual thiophenol upon workup was found to inhibit the subsequent Sc 3 -promoted hydroxymethylation.

大量实验揭示若干问题,随着几个对策 1鉴于锂金属溶化慢慢液氨、需要相当多的时间创造了金属溶液溶解(约占1小时);(2) T恤使操作必须事先用新鲜蒸馏水形成酮减少2119(图2);有效捕获的锂烯醇(11)使用新鲜蒸馏水需氯和ET三氮;含有残余硫workup经发现抑制随后推选3-羟甲晋升。

Methods A total of 64 round wounds (diameter of 6 mm each) with total skin loss were made on the ventral side of rabbit ear using a trephine. Morphology and collagen metabolism of scar wounds were studied at 14,21,35,70 and 98 days after operation, respectively.

用圆形钻刀在兔耳腹面产生直径6 mm的全层皮肤缺损创面64个,分别在术后14、21、35、70和98 d进行形态学观察与羟脯氨酸含量测定。

Objective: To set the measuring method of tyrosine hydroxylase activity in the brain of conscious rats.

中文摘要:目的:建立清醒自由活动大鼠脑内酪氨酸羟化酶活性的测定方法。

Studies also indicated that genistein exsists not only in soybeans but also in many kinds of plants such as vegetables and fruits.As a potential anticancer agent, the actional mechanisms of genistein mainly includes as follows:First, genistein can depress the activity of protein tyrosine kinase and transduction pathways for the phosphorylation of receptors and mitosis signal. So genistein can lead to cells' proliferation depressed. Second, genistein has minimal effects of phytoestrogens. It can be combined with estrogen receptor and improve the synthesis of cellular sex hormone binding glulobin, and improve the activity of UDP-glucuronyl transferase. Through these pathways, it can inhibit the cell activity of breast cancer and prostate cancer.

作为一个很有潜力的抗肿瘤物质,三羟异黄酮的作用机制主要包括:①抑制蛋白酪氨酸激酶活性,可阻抑PTK引起的受体磷酸化和有丝分裂的信号传递,导致癌细胞增殖受抑;②弱雌激素作用:可通过与体内雌激素受体结合,并可增加细胞内性激素结合球蛋白的合成,增加UDP-葡糖醛酸转移酶的活性等途径抑制乳腺癌和前列腺癌细胞活性;③拓扑酶Ⅰ和Ⅱ抑制剂,抑制细胞活性;④上调细胞周期性负性调节因子P21WAF1/CIP1的表达,使之负性调节因子作用增强;⑤可阻止胰岛素样生长因子-Ⅰ、肝细胞生长因子和神经生长因子的作用而抑制肿瘤生长;⑥其他:抗氧化作用、抑制热休克蛋白、诱导细胞凋亡、抑制新生血管生成和抑制多种耐药相关蛋白等。

The alveolitis and pulmonary fibrosis were examined by HE staining. The serum HYP was examined .The expression of CTGF was tested by immunohistochemical assay.

HE染色检测肺组织肺泡炎及肺纤维化程度;测定血清羟脯氨酸的含量;免疫组化染色检测CTGF的表达。

The taurohyodeoxycholic acid was synthesized by aminolysis of sodium taurine with carboxy-carbonate mixed anhydride prepared by reacting hyodeoxycholic acid with ethyl chloroformate at the present of triethylamine.

猪去氧胆酸与氯甲酸乙酯在三乙胺存在下反应生成羟酸-碳酸乙酯混合酐,而后将其与牛磺酸钠进行氨解反应,合成猪磺去氧胆酸。

Objective After Intense pulsed light irradiation cavia cobaya skin, to evaluate the histological change of skin with HE stains; to evaluate the collagen fiber change with nitroxanthic acid stains; to quantitative analysis of hydroxyproline content. Confirm mechanism of action for Intense pulsed light on sun-damaged human skin.

目的 用强脉冲光照射豚鼠背部皮肤后,通过HE染色观察皮肤组织学的改变;用苦味酸染色观察皮肤胶原纤维的改变;定量分析豚鼠皮肤羟脯氨酸的含量,观察皮肤胶原蛋白的变化,进一步研究强脉冲光治疗皮肤光老化的作用机制。

Methods 60 female mice were randomly divided into 4 groups (n=15):Mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of D-galactose[1000 mg/(kg1·d)] for 42 d to establish subacute aging model.

60只雌性昆明种小鼠,随机分为4组(n=15):正常对照组皮下注射生理盐水;其余3组每日颈背部皮下注射 D-半乳糖[1000 mg/] 造成小鼠衰老模型,同时各组分别灌胃生理盐水、枸杞多糖[200 mg/]、丹参酮[1 500 mg/]。42 d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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