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Isolation, cultivation and identification of human embryonic NSC from cerebral cortex of 7-12 week abortus. By using flow cytometry and MTT assay, the effects of various concentration of TSPG and TSPG cooperating with cytokines in NSC culture media for 3 days on proliferation of human embryonic NSC has studied. By employing immunocytochemistry assay of the expression of tyrosine hydroxylase, the effect of different dilution of TSPG and TSPG cooperating with IL-1 on induced differentiation of human embryonic NSC into dopaminergic neuron has researched.

从7~12周人工流产胚胎脑组织中分离培养人胚胎NSC,免疫细胞化学鉴定NSC特异性nestin抗原表达,在NSC的培养体系中加入TSPG,采用流式细胞术和MTT法检测不同浓度TSPG以及TSPG与EGF,bFGF联合应用对人胚胎NSC增殖的影响;经免疫细胞化学检测培养细胞酪氨酸羟化酶表达,分析不同浓度TSPG以及TSPG与IL-1联合应用对NSC定向诱导为DA能神经元的影响。

Hepatic pathology diagnosed them as mild and moderate steatohepatitis, and focal perisinous fibrosis developed in liver acinus's area 3 zone.

但不饱和脂肪酸组血浆ET、TNF-α明显高于饱和脂肪酸组,血浆HA、LN、TGF-β1及肝脏羟脯氨酸含量均显著高于饱和脂肪酸组。

At baseline and on day 1, 3, 7, 14, 21 following MPP+ injection, the degree of microglial activation was examined by detecting the immunodensity of OX-42 in the substantia nigra. The number of viable dopaminergic neurons was determined by measuring tyrosine hydroxylase positive neurons in the SN. Behavioral performances were evaluated by counting the number of rotations induced by apomorphine, calculating scores of forelimb akinesia and vibrissae-elicited forelimb placing asymmetry.

分别在基线、MPP+注射后第1、3、7、14、21天时通过测定黑质区域OX-42的免疫荧光强度评定小胶质细胞的激活程度,测定酪氨酸羟化酶的表达情况评定存活多巴胺神经元的数量;通过阿朴吗啡诱导的旋转行为、前肢跨步及触须引发的不对称放置试验得分测评行为学表现。

Amino acids of brain including glutamine,aspartic acid,almandine and Γ-aminobutyric acidwere assayed by amino acid analytic apparatus.

而在正常大鼠脑神经纤维网发育和髓鞘形成过程中,五羟色胺(5-HT)、苯丙氨酸的变化与很多脑疾病有关[1]。

A method of capillary electrophoresis with tris (2,2′-bipyridyl) ruthenium electrochemiluminescence detection has been applied to the simultaneous determination of proline and hydroxyproline in human urine.

1引言胶原蛋白中常含有丰富的脯氨酸和羟脯氨酸,其中Hyp几乎为胶原蛋白所特有。在胶原蛋白代谢中,Pro通过羟基化形成Hyp,胶原蛋白降解释放的Hyp从尿中排泄[1]。

It contains the amino acids needed by the body, such as alanine, arginine, glutamic acid, amion acetic acid, oxyproline, etc. which are very important to the parenchyma, skin, etc. of the body. More importantly, its molecular structure is from very minute to 1000 daltons, so it can be absorbed by the body easily to bring the due effect in the body.

富含涵盖体内所需的氨基酸如:丙胺酸、精氨酸、谷氨酸、氨基乙酸、羟脯氨酸等,对人体软组织、皮肤等非常重要的氨基酸,更重要的是拥有极度微小至1000道尔顿的分子结构,易于被人体吸收并在体内发挥达到应有的效果。

Hydroxy praline – The main sources of hydroxyl praline are carrots, lettuce, beetroots, turnips, cucumber, plums, cherries, figs, radishes, grapes, olives, pineapples, almonds and coconut.

羟基脯氨酸-的主要来源羟脯氨酸是胡萝卜,生菜, beetroots ,萝卜,黄瓜,李子,樱桃,无花果,萝卜,葡萄,橄榄,菠萝,杏仁和椰子。

Firstly, synthetic racemates of β- amino acids were resolved into their enantiomers by ehiral ligand - exchange flash chromatography with L - hydroxyproline chemically bonded to irregular silica as the stationary phase and aqueous solution containing cupric ions as the mo

首先合成L-羟脯氨酸硅胶键合手性固定相,用于快速色谱中3-羟基-4-甲氧基-β-苯丙氨酸对映体的制备分离,考察了流动相中Cu^2+浓度、样品体积等因素对分离的影响。

Yi TAI Compounded Medication "we used bases on the basic theory of TCM,set up "The treatment of invigoratmg spleen and promoting blood by regulating qi",target defiency in origin and excess in superficiality TCM syndrome nature,and take experiments;our research injecting L-Arg into abdomen to induce Spleeh-asthenis rat model,it is a new model which combine diseases and syndromes;immunohistochemic 、 optical mirror and biochemistry value number index number are used to detect the expression of related constrain factor TGF-β_1 mRNA、α-SMA when textnre fabrosis occurs in pancreatic,as nell as significance and relation of hydroxyproline in the process of pancreatic fibrosis,meanwhile observe the disturbace which "Yi TAI Compounded Medication makes,revel the anti-fibrosis mechanism from organization form and molecular level.

本研究采用L—精氨酸分次腹腔注射诱导脾虚型慢性胰腺炎大鼠模型,是一种病证结合的新模型;用免疫组化、光镜等技术和生化指标检测CP时胰实质纤维化的相关调控因素TGF-β_1、α-SMA的表达和血清中羟脯氨酸在胰腺纤维化过程中的作用及相互关系,同时观察了&胰泰复方&对其干预机理,从组织形态学和分子水平揭示了该方抗纤维化的作用机制。

To observe the effects of HASF on viability and collagen synthesis of two cell lines, AgNORs staining, 3H-proline incorporation and HYP determination were carried out.

层析法从兔羊水及胎兔血清中提取HASF并做活性鉴定;用嗜银蛋白染色法、3H-脯氨酸掺入法和羟脯氨酸测定法观察不同浓度的HASF对细胞增殖活性和胶原蛋白合成的影响。

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这一模式非常关注商人的网络信用基础。

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