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In vivo experimental results revealed that PAP 10 and 20 mg.kg-1 could promote healing of radius fracture,accelerate osteotylus formation and increase contents of calcium and oxyproline.

整体鹿茸多肽能明显加速骨痂的形成及骨折的愈合,明显增加骨痂内羟脯氨酸和钙含量。

The CFb in blank control group was added with IMDM containing 10% FBS and those in Ang Ⅱcontrol group with Ang Ⅱ.The CFb in the 3 test groups were induced with Ang II,then added with Tau at dosages of 40,80 and 160 mmol/L respectively. Determine the proliferation of CFb by MTT method, the collagen content by oxyproline kit, the transforming growth factor- TGF-β1 (TGF TGF-β1) content by ELISA, the cell cycle by flow cytometer, the CFb NO content by nitrate reductase method, the iNOS activity by spectrophotometry, and the expression of iNOS protein by IFA.

胰酶消化法分离培养新生大鼠CFb,用Ang Ⅱ诱导促进其增殖,采用MTT法检测细胞增殖;羟脯氨酸试剂盒检测胶原含量;ELISA法测定转化生长因子β1(TGF-β1)蛋白的含量;流式细胞仪检测细胞周期;硝酸还原酶法、分光光度法和免疫荧光法检测CFb NO含量、一氧化氮合酶活性和iNOS蛋白的表达。

Cycloserine was produced using L-serine methyl ester hydrochloride as raw material. L-serine methyl ester hydrochloride was chloridized with phosphorus pentachloride to afford 2-amino-3-chloride propanic acid methyl ester hydrochloride, which was cyclized with hydroxylamine in the presence of alkali to give cycloserine.

以L-丝氨酸甲酯盐酸盐为原料制备了环丝氨酸。L-丝氨酸甲酯盐酸盐和五氯化磷在氯仿溶剂中进行氯代反应生成2-氨基-3-氯丙氨酸甲酯盐酸盐,后者和羟胺在碱性溶液中发生环合反应得到环丝氨酸。

In the second experiment, we would like to understand the relationship between sexual performance and sexual motivation. The partner preference test was conducted to measure sexual incentive motivation, and tyrosine hydroxylase immunohistochemistry was used to assay the dopaminergic neuron activity in the medial preoptic area.

在第二部份实验所探讨的是雄鼠的性动机与性表现两者之关系,本实验以EGb 761作为一实验工具,利用伙伴偏好试验测试雄鼠性诱因动机,并利用酪氨酸羟化酶(tyrosine hydroxylase, TH)免疫化学染色方法分析内侧视前区多巴胺神经元的活性。

The reverse transcription-polymerase chain reaction was used to seek the expressions of a specific fibroblast marker, prolyl 4-hydroxylase β, and a specific marker of epithelial cells, cytokeratin-4, in the cells.

采用逆转录多聚酶链反应检测hEFs表达成纤维细胞特异性标志(脯氨酰4-羟化酶β亚单位)和上皮细胞特异性标志(细胞角蛋白4)的情况,对该细胞进行鉴定。

Inhibition of prolyl hydroxylase activity or expression can be effected using competitive inhibitors such as dimethyloxalylglycine or siRNA leading to the stabilization of HIF and other beneficial effects.

DMOG是一种脯氨酰羟化酶抑制剂,能够抑制细胞内HIF-1α的降解,继而使细胞核内很多基因(如EPO、VEGF、Ang1和Glut-1等)的转录和表达增高,继而发挥其保护性作用。

Results The hEFs were successfully isolated and cultivated from gonadal ridges and dorsal mesenteries of human embryos. They could be passaged beyond the 25th generation. The biologic characteristics of the cells did not change, even in high-passage cells or frozen-thawed cells. The cells expressed prolyl 4-hydroxylase β, but not cytokeratin-4, which was similar to the fibroblasts. The cultured cells expressed bFGF and LIF.

结果:从人胚胎生殖腺嵴和肠背系膜中成功地分离培养出hEFs,该细胞可传25代以上,且经过传代及冻存复苏后生物学特性无改变;hEFs表达脯氨酰4-羟化酶β亚单位,不表达细胞角蛋白4,确定其为成纤维细胞;该成纤维细胞表达bFGF和LIF。

At the fifth day after BTH, SA and SiO2 spraying, seedlings were inoculated with Sphaerotheca fuliginea, and disease indexes, leaf lignin and HRGP contents were determined for four times at alternate days.

以抗白粉病甜瓜品种'银帝'和感病品种'卡拉克赛'为材料,用BTH、SA和SiO2溶液分别预处理,5 d后接种白粉病菌并分4次调查处理植株的发病情况、测定叶片细胞壁中木质素和羟脯氨酸糖蛋白含量。

Ingredients: Water,Stearyl Trimethyl Ammonium Chloride,Cetearyl Alcohol,Cetyl Alcohol,Amodimethicone,Rosa Rugosa Flower Extract,Cyclomethicone,Dimethicone,Hydroxyethyl Cellulose,Hydrolyzed Keratin,Hydrolyzed Wheat Protein,Parfum,Methyl Paraben,Propyl Paraben,Methylchloroisothiazolinone Methylisothiazolinone,Citric Acid.

成分:去离子水,十八烷基三甲基氯化铵,鲸蜡硬脂醇,鲸蜡醇,氨二甲基硅氧烷,玫瑰花提取液,环甲基硅氧烷,聚二甲基硅氧烷,羟乙基纤维素,水解角蛋白,水解小麦蛋白,香精,对羟基苯甲酸甲酯,对羟基苯甲酸丙酯,凯松,柠檬酸。

Results: The Fos-like immunoreactive neurons distributed mainly in the spinal trigeminal subnucleus caudalisipsilaterally and medullary visceral zone (MVZ,including nucleus tractus solitarius,ventrolateral medulla and the reticular formation between the first two),ln the sections processed for double staining of Fos and TH immunohisto chemistry, numerous Fos-LI neurons showed also TH- like immunoreactivity in their cytoplasm(Fos-TH cells)in MVZ.

结果:Fos样免疫反应神经元主要分布在同侧三叉神经脊束核尾侧亚核、双侧延髓内脏带(孤束核、腹外侧区及二者之间的网状结构)。在抗Fos 和抗酪氨酸羟化酶的双重免疫染色切片上,见到延髓内脏带内许多Fos阳性神经元的胞浆同时呈TH样免疫反应(Fos-TH双重阳性细胞)。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

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