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Objective: To investigate acusing zu san li's influence on the content of hydroxyproline and Malondialdehyde in the skin of hireless photoageing mice as well as the activity of Superoxide Dismutase in their skin,and to investigate milkvetch's effect on the structure of the hireless photoageing mice.

目的:本项实验旨在研究针刺足三里对紫外线照射引起的无毛小鼠皮肤光老化模型皮肤组织羟脯氨酸含量、丙二醛含量、超氧化物歧化酶活力,以及无毛小鼠真皮结构变化的影响。

In the pretreatment group, Bak Foong Pill extracts were given for 14 days prior to MPTP. Immunostaining of tyrosine hydroxythase was used to observe the damage of dopamine neurons, and of Bax to detect the protein levels of Bax. High performance liquid chromatography-electrochemical detection was used to investigate the contents of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic in the Str.

给予BFP预处理后,用酪氨酸羟化酶免疫组织化学法观察黑质神经元的变化;同时应用免疫组织化学法检测Bax蛋白表达;应用高效液相-电化学方法检测纹状体巴胺及其代谢产物二羟基苯乙酸和高香草酸的含量变化。

Methods The effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 7-nitroindazole (7-NI) on nitric oxide synthase activity, levels of dopamine、3,4-dihydroxyphenylacetic acid、homovanillic acid, and TH-immunostained postive nerve fibres in the striatum of mice were determined by using colorimetic analysis, HPLC with electrochemical detection, and immunohistochemical analysis.

用比色分析、高效液相色谱电化学及免疫组化法检测1-甲基-4-苯基-四氢吡啶和7-硝基吲唑(7-NI)对C57BL小鼠纹状体一氧化氮合酶活性,多巴胺、二羟基苯乙酸、高香草酸水平和酪氨酸羟化酶免疫阳性神经纤维的影响。

In the pretreatment group, Bak Foong Pill extracts were given for 14 daya prior to MPTP. Immunostaining of tyrosine hydroxythase was used to observe the damage of dopamine neurons, and of Bax to detect the protein levels of Bax. High performance liquid chromatography-electrochemical detection was used to investigate the contents of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic in the Str.

给予BFP预处理后,用酪氨酸羟化酶免疫组织化学法观察黑质神经元的变化;同时应用免疫组织化学法检测Bax蛋白表达;应用高效液相-电化学方法检测纹状体多巴胺及其代谢产物二羟基苯乙酸和高香草酸的含量变化。

Ten rats in each group were randomly sacrificed 14 and 28 days after intratracheal instillation.

强的松组摘要:动物体重减轻80%( P <0.01),肺胶原蛋白降低35.3%( P <0.01),羟脯氨酸含量降低44.2%( P <0.01)。

Ten rats in each group were randomly sacrificed 14 and 28 days after intratracheal instillation.

强的松组:动物体重减轻80%( P <0.01),肺胶原蛋白降低35.3%( P <0.01),羟脯氨酸含量降低44.2%( P <0.01)。

Ten rats in each group were randomly sacrificed 14 and 28 days after intratracheal instillation.

结果 观察28天后,对照组:动物体重增加71%( P <0.05),肺胶原蛋白含量提高17.7%( P <0.05),过氧化脂质含量提高1.86倍( P <0.05),羟脯氨酸含量提高24.01%( P <0.05)。

Except for control group, rats in the other groups were intratracheally administered with bleomycin. Animals in pulmonary fibrosis model groups were sacrificed on day 3, 7, 14, 28 and 56. The sections of the right lung were stained by HE, Masson and sirius red.

除N组外,其余各组采用气管内注入BLM致大鼠肺纤维化模型,分别于3、7、14、28、56 d处死各组大鼠,右肺行苏木精-依红、Masson胶原及天狼猩红染色,测定左肺羟脯氨酸的含量。

5 And 5.0 mg/kg bleomycin were given intratracheally to every 15 mice in the bleomycin group, respectively. The mice in the control group were administrated the same volume of normal saline. Twenty-eight days after model establishment, all mice were sacrificed. Their left pulmonary lobe tissues were removed from mice and used for pathological section observation, and the right pulmonary lobe tissues were used for the hydroxyproline contents measurement. MAIN OUTCOME MEASURES: The survival condition of mice in 28 days.

60只小鼠随机数字表法分为博莱霉素组(n=45)和对照组(n=15),博莱霉素组各选15只动物分别气管内注射不同剂量(2,3.5,5 mg/kg)的博莱霉素,对照组气管内注射相同体积的生理盐水,所有小鼠于造模后第28天处死并取其左肺叶制作病理切片,右肺叶作羟脯氨酸含量测定。

Ab initio and density functional theory (B3LYP) in G98 were employed to study the following four direct aldol reactions between acetone and isobutyraldehyde or 3,3-dimethylbutyric aldehyde or benzaldehyde or 4-bromo-phenyl aldehyde catalyzed by-proline .

中文摘要:本文采用G98程序包中的从头计算方法和密度泛函方法计算研究了-脯氨酸催化丙酮与异丁醛、3,3-二甲基丁醛、苯甲醛及对溴苯甲醛的不对称直接羟醛缩合反应,共计四个反应体系。

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