羟基胺

The determination of amino acids in blood clam antianaemia oral liquid using HPLC-AccQ-Tag method is reported. The oral liquid reacted with 6-aminoquinolyl-N-hydroxysuccini-mdyl carbamate. Then, the corresponding derivatives were analyzed with an HPLC. The chromatographic conditions were AccQ-TagTM C18 column for amino acids analysis (3.9mm×15cm); mobile phase of program elution sodium acetate solution(pH5.02), acetonitrile, Milli-Q water. The amino acids'AQC deriatives were detected at 248nm with a UV-detector. Eighteen kinds of amino acids were completely determined in 35 minutes.

以6-氨基喹啉-N-羟基琥珀酰亚胺基氨基甲酸酯为衍生剂，与毛蚶抗贫血口服液中的氨基酸柱前定量衍生，用WatersHPLC仪，AccQ-TagTM专用C18柱(3.9mm×15cm)，以140mmol·L-1的醋酸钠溶液(pH5.02)为溶剂A，乙腈为溶剂B，超纯水为溶剂C，进行梯度洗脱，检测波长为248nm，35min测试完毕。

To dye the different types of fibers by the same dye, universal dyes based on naphthalene ring system are prepared by the diazo coupling reaction of 7-acetamide-4-hydroxy-2-oaphtialene-β-(N,N-dimethylaminoethyl)solfonamide and m-aminobenzene-β-hydroxyethyl sulfone sulfunate.

为了实现用同一支染料对不同类型的纤维进行染色的目的，通过间氨基苯基－β-羟乙基砜硫酸酯与7-乙酰氨基－4-羟基－2-萘－β磺酰胺的重氮化偶合反应合成了含萘环的通用染料，其结构通过质谱分析进行了表征。

The preparation and application of reactive antibacterial agents, such as silicone quaternary ammonium salt, halogenated hydroxy diphenyl ether, chitosan, halamine and other kinds, etc, were introduced in detail based on the art and development trend both here and abroad.

文章针对现有反应性抗菌整理剂的国内外发展现状和趋势，详细介绍了有机硅季铵盐、卤代羟基二苯醚、壳聚糖、卤胺及其他类等反应性抗菌整理剂的制备过程和应用情况，并对其应用前景作了展望。

The environmental black direct dye stuff without carcinogensis was prepared by 4,4'-diamino-dibenzidine-2-sulfonicacid,1-hydroxy-7-aminonaphthalene-3,6-dusuifonicacid,m-benzoic-diamine and aniline.

以4，4'-二氨基-二苯胺-2-磺酸代替苯胺，与1-羟基-7-氨基-3，6-二萘磺酸、间苯二胺、苯胺等合成无致癌作用的环保型黑色直接染料。

The environmental black direct dyestuff without carcinogensis was prepared by 4,4＇- diamino - dibenzidine - 2 - sulfonicacid, 1 - hydroxy - 7 - aminonaphthalene - 3,6 - dusuifonicacid, m - benzoic - diamine and aniline.

以4，4'-二氨基-二苯胺-2-磺酸代替苯胺，与1-羟基-7-氨基-3，6-二萘磺酸、间苯二胺、苯胺等合成无致癌作用的环保型黑色直接染料。

Then we completed the first total synthesis of-Plakoridine A using Eschenmoser couping reaction as akey step. During the synthesis we found the choice of protecting groups and the configuration of the chiralcenter of the substrate play an important role to the key Eschenmoser reaction,so we think our successwould be of guide for synthesizing other polysubstituted pyrrolidines or piperidines.

在论文的第一章中，我们先以易得的对羟基苯乙酸乙酯为原料经多步反应制得了所需的β氨基酸酯，然后利用立体选择性的Aldol缩合反应和内酰胺化反应制得了Plakoridine Alactam，随后，以Eshenmoser缩合反应为关键步骤完成了生物碱-Plakoridine A的首次不对称全合成。

Methods The amino acids in the injections were pretreated with 6-aminoquinolyl-N-hydroxysuccinimdyl separately and their derivatives were analyzed on Waters 2695 HPLC using AccQ-Tag column. Gradient eluents consisted of sodium acetate solution (pH 4.95), acetonitrile and water. The Detection wavelength was 248 nm and column temperature 37℃.

采用AccQ-Tag法，以6-氨基喹啉－N-羟基琥珀酰亚胺基甲酸酯为衍生试剂，与复方氨基酸注射液(18AA-V)中17种氨基酸柱前衍生，用Waters 2695 HPLC仪器，AccQ-Tag柱，以醋酸钠(pH 4.95)缓冲液为流动相A，乙腈为流动相B，水为流动相C进行梯度洗脱，检测波长为248 nm，柱温为37℃，进样量为5μL。

ES cells were maintained on gelatin-coated dishes in Iscove's modified Dulbecco medium containing 0.1 mmol/L of nonessential amino acids, 2 mmol/L of L-glutamine, 0.1 mmol/L of monothioglycerol, 15% fetal bovine serum, and 1000 U/mL leukemia inhibitory factor. Embryonic bodies were firstly formed by ES cells with hang drip method in IMDM without LIF. Six-day-old EBs were trypsinized and the cells were resuspended at a concentration of （3-4）×109 L-1 cells per 1 mL of a 1:1 mixture of culture medium and Matrigel?

实验方法：将鼠胚胎干细胞株接种于被覆明胶的培养瓶内，加入含0.1 mmol/L非必需氨基酸、2 mmol/L L-谷酰胺、0.1 mmol/L二羟基丙硫醇、15%胎牛血清、1 000 U/mL白血病抑制因子的IMDM培养液。3 d后采用&悬滴法&使细胞在不含白血病抑制因子的上述IMDM培养液中形成胚胎体，6 d后胰酶消化得到胚胎干细胞，浓度调整为（3~4）×109 L-1，与Matrigel？

The reaction mechanism of tetrakis hydroxymethyl phosphonium chloride and raw collagen were researched by the simulated materials of collagen including glycin,ethylenediamine and hide powder.

通过研究四羟甲基氯化鏻与皮胶原模拟物反应，间接证明THPC主要与胶原蛋白的氨基结合，可与氨基以n∶n=1∶2.2形成交联，THPC与羟基和酰胺基均有少量结合，并在pH=8.0时结合量最高。

The novel synthesis of the 2-hydroxyl α-arylglycines was realized through the Lewis acids promoted Friedel-Crafts reaction of phenols and N-tosyl imino ester in mild reaction conditions.

其中在路易酸TiCl〓促进下，酚类化合物和亚胺酯发生的傅克反应，可较高产率、较高区域选择性的得到2-羟基α-芳香甘氨酸衍生物。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。