羟基化

The method involves exposing the cells to a compound having the formula I in which: w is a nucleic acid x is a non-amino acid or non-peptide nucleic acid binding group y is a spacer having a chain length equivalent to 1-30 carbon-carbon single covalent bonds or is absent R4 is H or halogen or CH2O-R3; and R1, R2 and R3 are the same or different and are either hydrogen, methyl, ethyl, alkyl, alkenyl, hydroxylated alkyl, hydroxylated alkenyl groups or ether containing alkyl, alkenyl, hydroxylated alkyl or hydroxylated alkenyl groups optionally being an acyl group having a carbon chain length equivalent to 3-24 carbon atoms saturated or unsaturated, with the proviso that at least one of R1, R2 or R3 includes a group having a carbon chain of 3-24 carbon atoms saturated or unsaturated, or to a compound having the formula II in which: w is a nucleic acid x is a non-amino acid or non-peptide nucleic acid binding group y is a space having a chain length equivalent to 1-30 carbon-carbon single covalent bonds or is absent, R5 is alkyl, alkenyl, hydroxylated alkyl, hydroxylated alkenyl group or ether containing alkyl, alkenyl, hydroxylated alkyl or hydroxylated alkenyl group optionally being an acyl group having a carbon chain length equivalent to 3-24 carbon atoms saturated or unsaturated, with the proviso that R5 includes a group having a carbon chain of 3-24 carbon atoms saturated or unsaturated.

该方法包括使细胞暴露于具有式的化合物，在式中：w是核酸，x是非氨基酸或者非肽核酸结合基团，y是具有等于1―30个碳―碳单共价键的链长的间隔基或者不存在，R4是H或者卤素或者CH2O－R3；R1、R2和R3是相同的或者不同的并且是氢、甲基、乙基、烷基、链烯基、羟基化烷基、羟基化链烯基或者包含烷基、链烯基、羟基化烷基或羟基化链烯基的醚，任选地是来源于具有等于3―24个碳原子的碳链长的饱和或不饱和脂肪酸的酰基，其条件是R1、R2或者R3的至少一个包括具有3―24个碳原子的饱和或不饱和碳链的基团，或者使细胞暴露于具有式的化合物，在式中：w是核酸，x是非氨基酸或者非肽核酸结合基团，y是具有等于1―30个碳―碳单共价键的链长的间隔基或者不存在，R5是烷基、链烯基、羟基化烷基、羟基化链烯基或者包含烷基、链烯基、羟基化烷基或羟基化链烯基的醚，任选地是具有等于3―24个碳原子的饱和或不饱和碳链的链长的酰基，其条件是R5包括具有3―24个碳原子的饱和或不饱和碳链的基团。

There six chapters in this dissertation: the overview on the study of probing for reactive oxygen species, spin-labelled fluorophore as probe for free radicals, Fe -EDTA chelate-induced aromatic hydroxylation of terephthalate as a new method for the evaluation of hydroxyl radical-scavenging ability, study of fluorimetric method for the determination of peroxynitrite, study of spiro-formed rhodamine B hydrazide as a novel fluorescent probe in analytical chemistry, the conclusion and prospect of the present research work.

本论文分六章，包括生物活性氧表征的研究进展、自旋标记荧光探针表征自由基的研究、利用Fe-EDTA引发对苯二甲酸芳环羟基化反应评价抗氧化剂清除羟基自由基能力的研究、荧光法表征过氧亚硝酸根的研究、螺环型罗丹明B酰肼化合物作为新型荧光探针的性质及应用的研究、结论与展望。

Strong acids, such as sulfuric acid, hampered from the occurrence of the phenol hydroxylation.

过强的酸，如硫酸、硝酸和盐酸，对苯酚羟基化反应造成危害，使羟基化反应不能发生。

At the condition of shake flask fermentation,the metabolic characters of Bacillus pumilus in oleic acid were studied using proteose as the main nitro source and glucose as the carbon source.

一株具有转化油酸羟基化的微生物Bacillus pum ilus BD-12(记作BP BD-12)，在摇瓶条件下分批深层培养，研究了菌株在以胰蛋白胨为主要氮源、葡萄糖为基本碳源的条件下对油酸的代谢特性，并对转化OA生成羟基脂肪酸的规律及产物进行了分析测定。

Research and development for fhe production of pyrocatechol and hydroquinone by reaction of phenol with hydrogen peroxide and percarboxylic acid as hydroxylating reagent are introduced.

着重介绍用过氧化氢和过氧化羧酸作羟基化剂，使苯酚羟基化生成邻苯二酚和对苯二酚的研究开发进展。

The bacterial, mammalian and plant cytochrome P450s involved in metabolism of herbicides are reviewed in this paper. The action modes of cytochrome P450s on herbicides including dealkylation, ring-methyl hydroxylation and aromatic ring hydroxylation etc are summarized.

本文综述了代谢除草剂的细菌、哺乳动物和植物细胞色素P450酶系，概述了细胞色素P450酶系参与除草剂代谢的作用方式：脱烷基化作用、环甲基化羟基化作用和芳环的羟基化作用等。

The method comprises following steps: reacting hydroxylation carbon nano-tube successively with reagent containing halogen and hydrosulphide to prepare sulfhydryl carbon nano-tube; reflowing the sulfhydryl carbon nano-tube and platinum salts in polyatomic alcohol, processing in hydrogen atmpsphere to obtain sulfhydryl anchoring platinum / carbon nano-tube catalyst; reflowing the sulfhydryl carbon nano-tube successively with the hydrochloroauric acid and the platinum salts in polyatomic alcohol, processing in hydrogen atmpsphere to obtain sulfhydryl anchoring platinum-gold / carbon nano-tube catalyst.

本发明将市售羟基化碳纳米管先后与含卤试剂和氢硫化物反应得到巯基化碳纳米管。再将巯基化碳纳米管与铂盐在多元醇中回流，氢气氛中处理得到巯基锚固铂/碳纳米管催化剂。将巯基化碳纳米管依次与氯金酸和铂盐在多元醇中回流，氢气氛中处理得到巯基锚固铂-金/碳纳米管催化剂。

Green tea contains a number of flavonols with the basic structure Specific flavonols found in tea include myricetin (hydroxyls at carbon 3', 4', and 5'), quercetin (hydroxyls at positions 3' and 4'), and kaempferol (hydroxyl at position 4'), and their glycosides.

绿茶含有一些黄酮醇，其基本化学结构为：茶中特有的黄酮醇有杨梅黄酮(3'、4'、5'碳位上羟基化)、豕草花粉苷(3'和4'碳位上羟基化)，莰非醇(4'碳位上羟基化)，以及它们的糖苷物。

In the hydroxylation of phenol with H〓O〓 in the presence of Ti-ZSM-5, water is best solvent. It produces catechol and hydroquinone simultaneously, and catechol/hydroquinone ratio is approximately 0.85. The results show H〓O〓 utilization ratio increase 14%, when nonoxidizable weak acid HAc is added into the reaction system. Under the conditions of Ti-ZSM-5 as catalyst, phenol/H〓O〓 ratio being 10, a little HAc, temperature 75℃, reaction time 6h, H〓O〓 utilization ratio reaches 74. 6mo1% in the reaction.

对Ti-ZSM-5催化苯酚羟基化的研究结果表明，水是最好的溶剂，羟基化有邻、对苯二酚两种产物，并且邻苯二酚/对苯二酚摩尔比可维持在～0.85左右；向反应体系中加入非氧化性弱酸性助剂HAc时，H〓O〓有效利用率较未加入助剂时可提高14mol%；当催化剂用量为8g/100g苯酚、苯酚/H〓O〓比为10、反应温度75℃、添加少量HAc的条件下，反应6h后，H〓O〓有效利用率可达74.6mol%。

Triphenylmethyl chloride can react selectively with the 30-hydroxil group of glycyrrhetol under the catalyzing effects of three ethyl amine and dimethyl amino-pyridine.This condition of the reaction has an important value for the selective protection of 30-hydroxil group of glycyrrhetol.

三苯氯甲烷在三乙胺和二甲氨基吡啶等碱性催化下与甘草萜醇的C30-位羟基进行选择性缩合反应，此反应产率较与C3-位羟基化反应的要高，此种反应条件在甘草萜醇C30-位羟基的选择性保护中具有重要的价值。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。