羟基丙酮

Their structures were characterized as dihydroconiferyl dihydro-p-coumarate, vanillin, apocynin, p-hydroxybenzaldehyde, syringaldehyde, syringic acid, syringylethanone,α-hydroxypropiosyringone, coniferyl aldehyde, dihydroconiferyl alcohol, 2-hydroxyphenylpropanol, and 3-hydroxy-4-methoxy-phenylethanol, respectively.

结果 从该植物中分离得到12个酚性化合物，其结构分别为二氢松柏醇二氢对羟基桂皮酸酯，香草醛，罗布麻宁，对羟基苯甲醛，丁香醛，丁香酸，丁香乙酮，α-羟基丁香丙酮，松柏醛，二氢松柏醇，2-羟基苯丙醇和3-羟基－4-甲氧基苯乙醇。

The bio-oil was composed of acetic acid, hydroxyacetaldehy, acetol, levoglucosan, furaldehyde, and a small quantity of phenols.

结果表明，热解温度为460~520℃时热解油产率最大，主要有醋酸、羟基乙醛、羟基丙酮、左旋葡聚糖、糠醛，油热值为17.0MJ.kg-1；热解气主要为CO2和CO，还含有少量H2、CH4和C2~C4的烯烃。

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

In the second part of the thesis, we discussed a L-proline-catalyzed direct Aldol reaction between L-amino acid-derived N,N-dibenzyl -amino aldehydes 15 and acetone, cyclopentanone or hydroxyacetone, which afforded (-amino--hydroxy- or (-amino-,(-dihydroxy-ketones in good to excellent yields (up to 94%) and diastereoseletivities (up to 94.9%).

发现许多情况下，反应的产率和de值超过90%。接着，在碱性条件下，我们将L-亮氨酸衍生的N，N-二苄基--氨基醛15g与羟基丙酮反应得到的主要产物(，-二羟基-γ-氨基酮171d与次溴酸钠反应，得到了具有抗肿瘤活性的天然产物PM-94128的氨基酸片断184。

Artificial local tanning with a preparation containing dihydroxy acetone in alcohol can be useful.

当地人工晒黑与制备含有二羟基丙酮在酒精可以是有益的。

Two synthetic routes starting from 1,3-dihydroxyacetone and formamidine acetate as well as imidazole-4,5-dicarboxylic acid as starting material were investigated, and the reductive reaction of ethyl imidazole-4-carboxylate was also investigated.

本文对以1，3-二羟基丙酮和醋酸甲脒为原料和以4，5-二羧酸咪唑为原料合成4(5)-羟甲基咪唑的两条工艺进行了研究并优化，同时对4(5)-羧酸咪唑乙酯的还原反应进行了探索性实验。

The optimum process starting from 1,3-dihydroxyacetone and formamidine acetate was: 22.54g(0.25mol) 1,3-dihydroxyacetone, 29.12g(0.28mol) formamidine acetate and 120ml isopropanol in liquid ammonia was heated to 40℃ for 8h.

以1，3-二羟基丙酮和醋酸甲脒为原料的较佳工艺：1，3-二羟基丙酮22.54g (0.25mol)，醋酸甲脒29.12g (0.28mol)，异丙醇120ml，通入液氨，40℃反应8h，减压蒸馏除去反应液中的氨气后过滤，滤液加至热的苦味酸饱和溶液，自然冷却至室温，制得4(5)-羟甲基咪唑苦味酸盐69.5g，收率85.0%。

Dihydroxyacetone; formamidine acetate; imidazole-4,5-dicarboxylic acid; optimizing process; ethyl imidazole-4-carboxylate; reductive reaction; 4(5)-hydroxymethylimidazole

关键字或关键词组： 1，3-二羟基丙酮；醋酸甲脒；4，5-二羧酸咪唑；工艺优化；4(5)-羧酸咪唑乙酯；还原反应；4(5)-羟甲基咪唑

The products were identified by IR,NMR,MS and elemental analysis. In our method,K.OH was used in place of NaOH to synthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate was purified on a silica gel column with chloroform/ethylacetate(4:l,v/v) as eluent and further purified by triturating with hexane/acetone(2:l,v/v).The luminescence produced by DMAE-NHS is a flash light with maximumemission at 0.4s and decay half-time of 0.9s. The luminescence intensity is 6.11x10 cps/mol,which is affected by the composition of trigger and surfactant.

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿/乙酸乙酯(4：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，第二次柱层析用己烷/丙酮(2：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷/丙酮(2：1，Ⅴ：Ⅴ)进行研磨，过滤，洗涤，除去溶于己烷/丙酮(2：1，Ⅴ：Ⅴ)的部分，得到所需要的产品。

The reaction catalyzed by DAK converts DHA into dihydroxyacetone phosphate which is no toxic and can be ustilized by yeast cells. DAK presents in most organisms. DAK genes in the Saccharmyces cerevisiae and P. pastoris were cloned and characterized by a combined genetic and biochemical approach and the proteins encoded by these DAK genes are homodimer.

二羟基丙酮对酵母细胞来说是种有毒的化合物，在酵母菌中二羟基丙酮激酶参与二羟基丙酮的脱毒作用，DAK催化的反应使二羟基丙酮磷酸化，形成无毒性的磷酸二羟丙酮，可为酵母细胞所利用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。