美洲

Pit boss walked off with it.11、I suggest you find a way to bury the hatchet with Michael Scofield's woman.

bury the hatchet 典故：17世纪，欧洲人移民美洲的初期，美洲本土的印第安部落首领以埋斧头的传统方式表示休战，而后就沿用到了英语中。

Pokeweed antiviral protein belongs to Ribosome-inactive Proteins. PAP has potent antiviral activity against many plant virus. pT1 was used as the template to amplify the deleted mutant PAP gene (N-deleted and N-,C-deIeted) by PCR and then the two genes were cloned into expression vector. Different IPTG-inducible expression vector containing different deleted mutant PAP gene was constructed and transferred into E.coli strain BL21 (DE3)-plysS.

美洲商陆抗病毒蛋白是一类能很好的抑制植物病毒的蛋白，很早就有报道低剂量的商陆叶片粗粉末就能明显的抑制TMV在菜豆叶片上局部枯斑的形成，PAP缓释剂也已用于病毒病的防治工作，但美洲商陆抗病毒蛋白属于核糖体失活蛋白，它对生长的细胞会有一定的毒性。

Pokeweed antiviral protein, a 29-kD protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60s subunit of eukaryotic ribosomes. In addition to its ribosome-inactivating ability, PAP has potent antiviral activity against many plants and animal viruses.

美洲商陆抗病毒蛋白(pokeweed antiviral proteins，PAPs)，是在美洲商陆的不同组织或不同生长阶段生成的一类具酶功能的单链核糖体失活蛋白(ribosome-inactivating proteins，RIP)，是天然的广谱抗病毒生物活性物资，能抗多种植物和动物病毒。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

Dry heat and steam baths had advocates in ancient Rome and pre-Columbian Americans used sweat lodges.

用干热浴发汗的方式在古罗马很受欢迎，而哥伦布发现美洲大陆前的美洲人则使用发汗小屋。

Puma , a kind of animal in American, is the topic of the first lesson.

美洲狮，一种美洲动物，是第一课的主题。

The town's same is reported to have several possibly origins including the old Ute Indian word for tumbleweed, the name of an Native American chief or "tu-wanda", the Goshute word for "bear."

根据报告Tooele镇的名字可能有多种命名来源，包括以美洲印第安尤特人语里的"风滚草"，美洲当地语言中的"tu-wanda"，Goshute语中的"熊"

Any of various North American vulture s, such as the turkey vulture .

美洲兀鹰，红头兀鹰北美秃鹫的一种，如红头美洲鹫

We released our young American players to play in the Copa America, and that's not a mandatory FIFA tournament.

我们让很多年轻的美国球员参加美洲杯，但美洲杯并不是国际足联强制要求的赛事。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。